Binding of western equine encephalomyelitis virus to brush border fragments isolated from mesenteronal epithelial cells of mosquitoes

Houk, Edward J.; Arcus, Yvonne M.; Hardy, James L.; Kramer, Laura D.

doi:10.1016/0168-1702(90)90072-j

Cited by 19 publications

(28 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, according to Mammen et al (1998) the translational entropy of a molecule is related to the natural logarithm of its mass. On the basis of the molecular weights of Bromegrass mosaic virus at 4.6 × 10 6 Da (Bockstahler and Kaesberg, 1962) and Semliki Forest virus at 6.5 × 10 7 Da (Houk et al, 1990) ΔS trans would be 6.5-fold and 9-fold higher, respectively, than that for fasciculin-2 at 6735 Da (Sigma-Aldrich, 2018). For the purpose of demonstration of the effect of ΔS a_immob here, ΔS a_immob is assumed to be 6-fold that for fasciculin-2 binding (Minh et al, 2005), i.e.…”

Section: Data For δS A_immob : Changes In Rotational and Translationamentioning

confidence: 99%

“…There are multiple Cr molecules on the cell surface of each susceptible host cell (Houk et al, 1990;Nunes-Correia et al, 1999;Schlegel et al, 1982) allowing the possibility of a single virion making multiple GP/Cr contacts on binding depending on their spatial proximity. The number of GP/Cr contacts per virion also depends on the number of GP molecules per virion and their spatial geometry.…”

Section: Data On Number Of Gp/cr Contacts Made During Virus/host Cellmentioning

confidence: 99%

“…Thus an adult female Culicoides variipennis biting midge is able to ingest ∼10 −4 ml (10 −10 m 3 ) (Fu, 1995) and larval tick stages ingest ∼1 µl (10 −9 m 3 ) while adult ticks may ingest ∼1 ml (10 −6 m 3 ) (Koch and Sauer, 1984). The luminal surface area of the mosquito midgut is 3 mm 2 (3.0 × 10 −6 m 2 ) (Houk et al, 1990) and assuming a spherical shape, the volume of the midgut in an engorged female mosquito would be 0.49 mm 3 (4.9 × 10 −10 m 3 ). Houk et al (1990) estimated 10,000 epithelial cells in the mosquito midgut, although Smith et al (2008) estimated that, of these, only 100 midgut cells in Ae.…”

Section: Thermodynamic Component Of Equation 7: Modelling CV T and Tmentioning

confidence: 99%

“…The luminal surface area of the mosquito midgut is 3 mm 2 (3.0 × 10 −6 m 2 ) (Houk et al, 1990) and assuming a spherical shape, the volume of the midgut in an engorged female mosquito would be 0.49 mm 3 (4.9 × 10 −10 m 3 ). Houk et al (1990) estimated 10,000 epithelial cells in the mosquito midgut, although Smith et al (2008) estimated that, of these, only 100 midgut cells in Ae. taeniorhyncus mosquitoes were actually susceptible to infection by VEEV even with high-titred blood-meals.…”

Section: Thermodynamic Component Of Equation 7: Modelling CV T and Tmentioning

confidence: 99%

See 3 more Smart Citations

Towards a thermodynamic mechanistic model for the effect of temperature on arthropod vector competence for transmission of arboviruses

Gale¹

2019

Microbial Risk Analysis

View full text Add to dashboard Cite

A B S T R A C TArboviruses such as West Nile virus (WNV), bluetongue virus (BTV), dengue virus (DENV) and chikungunya virus (CHIKV) infect their arthropod vectors over a range of average temperatures depending on the ambient temperature. How the transmission efficiency of an arbovirus (i.e. vector competence) varies with temperature influences not only the short term risk of arbovirus outbreaks in humans and livestock but also the long term impact of climate change on the geographical range of the virus. The strength of the interaction between viral surface (glyco)protein (GP) and the host cell receptor (Cr) on binding of virus to host cell is defined by the thermodynamic dissociation constant K d_receptor which is assumed to equal 10 −3 M (at 37°C) for binding of a sialic acid (SA) on the arthropod midgut epithelial cell surface to a SA-binding site on the surface of BTV, for example. Here virus binding affinity is modelled with increasing number of GP/Cr contacts at temperatures from 10°C to 35°C taking into account the change in entropy on immobilization of the whole virus on binding (ΔS a_immob ). Based on published data, three thermodynamic GP/Cr binding scenarios, namely enthalpy-driven, entropy-assisted and entropy-driven, are shown to affect the temperature sensitivity of virus binding in different ways. Thus for enthalpy-driven GP/Cr binding, viruses bind host cells much more strongly at 10°C than 35°C. A mechanistic model is developed for the number of arthropod midgut cells with bound virus and by building in a kinetic component for the rate of arbovirus replication and subsequent spread to the arthropod salivary glands, a model for the effect of temperature on vector competence is developed. The model separates the opposing effects of temperature on midgut cell binding affinity from the kinetic component of virogenesis. It successfully accommodates both increases in vector competence with temperature as for DENV and WNV in mosquitoes and decreases as for the CHIKV 2010-1909 strain in various populations of Aedes albopictus mosquitoes. Enhanced cell binding at lower temperatures through enthalpy-driven GP/Cr binding compensates for the lower replication rate to some degree such that some transmission can still occur at lower temperatures. In contrast, the strength of entropy-driven GP/Cr binding diminishes at low temperatures although there is no minimum temperature threshold for transmission efficiency. The magnitude of ΔS a_immob is an important data gap. It is concluded that thermodynamic and kinetic data obtained at the molecular level will prove important in modelling vector competence with temperature.Abbreviations: AIV, avian influenza virus; BBF, brush border fragments from midgut; C free , number of midgut epithelial cells which can bind virus with no virus bound; CHIKV, chikungunya virus; Cr, host cell receptor; C total_midgut , number of midgut epithelial cells which can bind virus; C.V T , number of arthropod midgut cells with bound arbovirus at temperature T; DENV, dengue fever virus; E A ...

show abstract

Section: Data For δS A_immob : Changes In Rotational and Translationamentioning

confidence: 99%

Section: Data On Number Of Gp/cr Contacts Made During Virus/host Cellmentioning

confidence: 99%

Section: Thermodynamic Component Of Equation 7: Modelling CV T and Tmentioning

confidence: 99%

Section: Thermodynamic Component Of Equation 7: Modelling CV T and Tmentioning

confidence: 99%

See 2 more Smart Citations

Towards a thermodynamic mechanistic model for the effect of temperature on arthropod vector competence for transmission of arboviruses

Gale¹

2019

Microbial Risk Analysis

View full text Add to dashboard Cite

show abstract

“…aegypti susceptibility to infection with DENV. Genes that have also been shown to play a role in mosquito competence include genes producing proteins that bind with an arbovirus [104,105,106,107,108,109,110,111,112,113]. A variety of Ae.…”

Section: Mosquito Genetic Factors Influence Mosquito Competence Fomentioning

confidence: 99%

Nature, Nurture and Evolution of Intra-Species Variation in Mosquito Arbovirus Transmission Competence

Tabachnick

2013

IJERPH

105

111

View full text Add to dashboard Cite

Mosquitoes vary in their competence or ability to transmit arthropod-borne viruses (arboviruses). Many arboviruses cause disease in humans and animals. Identifying the environmental and genetic causes of variation in mosquito competence for arboviruses is one of the great challenges in public health. Progress identifying genetic (nature) and environmental (nurture) factors influencing mosquito competence for arboviruses is reviewed. There is great complexity in the various traits that comprise mosquito competence. The complex interactions between environmental and genetic factors controlling these traits and the factors shaping variation in Nature are largely unknown. The norms of reaction of specific genes influencing competence, their distributions in natural populations and the effects of genetic polymorphism on phenotypic variation need to be determined. Mechanisms influencing competence are not likely due to natural selection because of the direct effects of the arbovirus on mosquito fitness. More likely the traits for mosquito competence for arboviruses are the effects of adaptations for other functions of these competence mechanisms. Determining these other functions is essential to understand the evolution and distributions of competence for arboviruses. This information is needed to assess risk from mosquito-borne disease, predict new mosquito-arbovirus systems, and provide novel strategies to mitigate mosquito-borne arbovirus transmission.

show abstract

Insect-transmitted pathogens in the insect midgut

Kaslow¹,

Welburn²

1996

Biology of the Insect Midgut

View full text Add to dashboard Cite

Binding of western equine encephalomyelitis virus to brush border fragments isolated from mesenteronal epithelial cells of mosquitoes

Cited by 19 publications

References 30 publications

Towards a thermodynamic mechanistic model for the effect of temperature on arthropod vector competence for transmission of arboviruses

Towards a thermodynamic mechanistic model for the effect of temperature on arthropod vector competence for transmission of arboviruses

Nature, Nurture and Evolution of Intra-Species Variation in Mosquito Arbovirus Transmission Competence

Insect-transmitted pathogens in the insect midgut

Contact Info

Product

Resources

About