Binding of Transmissible Gastroenteritis Coronavirus to Brush Border Membrane Sialoglycoproteins

Schwegmann-Weßels, Christel; Zimmer, Gert; Schröder, Bernd; Breves, Gerhard; Herrler, Georg

doi:10.1128/jvi.77.21.11846-11848.2003

Cited by 58 publications

(61 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This interaction is not only important for enhancing cell attachment and entry, but also increases the stability of the virus against detergent-like bile salts encountered in the gastrointestinal tract [27]. Furthermore, a role in overcoming the mucus barrier and intestinal peristalsis by binding of virions to Sia of mucin-type glycoproteins has been postulated [28].…”

Section: Coronavirusesmentioning

confidence: 98%

Sialic acid-specific lectins: occurrence, specificity and function

Lehmann

Tiralongo

2006

Cell. Mol. Life Sci.

202

194

View full text Add to dashboard Cite

Sialic acids consist of a family of acidic nine-carbon sugars that are typically located at the terminal positions of a variety of glycoconjugates. Naturally occurring sialic acids show an immense diversity of structure, and this reflects their involvement in a variety of biologically important processes. One such process involves the direct participation of sialic acids in recognition events through specific interactions with lectins, a family of proteins that recognise and bind sugars. This review will present a detailed overview of our current knowledge regarding the occurrence, specificity and function of sialic acid-specific lectins, particularly those that occur in viruses, bacteria and non-vertebrate eukaryotes.

show abstract

Section: Coronavirusesmentioning

confidence: 98%

Sialic acid-specific lectins: occurrence, specificity and function

Lehmann

Tiralongo

2006

Cell. Mol. Life Sci.

202

194

View full text Add to dashboard Cite

show abstract

“…Binding of TGEV to neuraminic acid increases the efficiency of binding to cultured cells, though it is not an absolute requirement [85]. That said mutants that were unable to bind to neuraminic acid were no longer enteropathogenic [59,86].…”

Section: Coronavirus Spike Protein As a Determinant Of Pathogenicitymentioning

confidence: 99%

Coronavirus avian infectious bronchitis virus

2007

View full text Add to dashboard Cite

-Infectious bronchitis virus (IBV), the coronavirus of the chicken (Gallus gallus), is one of the foremost causes of economic loss within the poultry industry, affecting the performance of both meat-type and egg-laying birds. The virus replicates not only in the epithelium of upper and lower respiratory tract tissues, but also in many tissues along the alimentary tract and elsewhere e.g. kidney, oviduct and testes. It can be detected in both respiratory and faecal material. There is increasing evidence that IBV can infect species of bird other than the chicken. Interestingly breeds of chicken vary with respect to the severity of infection with IBV, which may be related to the immune response. Probably the major reason for the high profile of IBV is the existence of a very large number of serotypes. Both live and inactivated IB vaccines are used extensively, the latter requiring priming by the former. Their effectiveness is diminished by poor cross-protection. The nature of the protective immune response to IBV is poorly understood. What is known is that the surface spike protein, indeed the amino-terminal S1 half, is sufficient to induce good protective immunity. There is increasing evidence that only a few amino acid differences amongst S proteins are sufficient to have a detrimental impact on cross-protection. Experimental vector IB vaccines and genetically manipulated IBVs -with heterologous spike protein genes -have produced promising results, including in the context of in ovo vaccination.

show abstract

“…21,22 The SA-binding activity of some coronaviruses (TGEV and IBV) helps the virus penetrate the mucus layer and pro- ceed to the intestinal enterocytes, where it interacts with pAPN to initiate infection. 23 To further explore effects on virus adsorption and determine whether the EtOH extract and five compounds can block virus adsorption and cell entry, we tested the effects of the EtOH extract and compounds 1-5 on PEDV-induced hemagglutination binding to rabbit red blood cells (rRBCs). 23 To further explore effects on virus adsorption and determine whether the EtOH extract and five compounds can block virus adsorption and cell entry, we tested the effects of the EtOH extract and compounds 1-5 on PEDV-induced hemagglutination binding to rabbit red blood cells (rRBCs).…”

Section: Hemagglutination Inhibition (Hi) Activitymentioning

confidence: 99%

In vitro antiviral activity of phlorotannins isolated from Ecklonia cava against porcine epidemic diarrhea coronavirus infection and hemagglutination

Kwon

Ryu

Kim

et al. 2013

Bioorganic & Medicinal Chemistry

View full text Add to dashboard Cite

Despite the prepdominat agent causing severe entero-pathogenic diarrhea in swine, there are no effective therapeutical treatment of porcine epidemic diarrhea virus (PEDV). In this study, we evaluated the antiviral activity of five phlorotannins isolated from Ecklonia cava (E. cava) against PEDV. In vitro antiviral activity was tested using two different assay strategies: (1) blockage of the binding of virus to cells (simultaneous-treatment assay) and (2) inhibition of viral replication (post-treatment assay). In simultaneous-treatment assay, compounds 2-5 except compound 1 exhibited antiviral activities of a 50% inhibitory concentration (IC₅₀) with the ranging from 10.8 ± 1.4 to 22.5 ± 2.2 μM against PEDV. Compounds 1-5 were completely blocked binding of viral spike protein to sialic acids at less than 36.6 μM concentrations by hemagglutination inhibition. Moreover, compounds 4 and 5 of five phlorotannins inhibited viral replication with IC₅₀ values of 12.2 ± 2.8 and 14.6 ± 1.3 μM in the post-treatment assay, respectively. During virus replication steps, compounds 4 and 5 exhibited stronger inhibition of viral RNA and viral protein synthesis in late stages (18 and 24 h) than in early stages (6 and 12 h). Interestingly, compounds 4 and 5 inhibited both viral entry by hemagglutination inhibition and viral replication by inhibition of viral RNA and viral protein synthesis, but not viral protease. These results suggest that compounds isolated from E. cava have strong antiviral activity against PEDV, inhibiting viral entry and/or viral replication, and may be developed into natural therapeutic drugs against coronavirus infection.

show abstract

Binding of Transmissible Gastroenteritis Coronavirus to Brush Border Membrane Sialoglycoproteins

Cited by 58 publications

References 14 publications

Sialic acid-specific lectins: occurrence, specificity and function

Sialic acid-specific lectins: occurrence, specificity and function

Coronavirus avian infectious bronchitis virus

In vitro antiviral activity of phlorotannins isolated from Ecklonia cava against porcine epidemic diarrhea coronavirus infection and hemagglutination

Contact Info

Product

Resources

About