Binding of the LXCXE Insulin Motif to a Hexapeptide Derived from Retinoblastoma Protein

Radulescu, Razvan T.; Bellitti, M.R.; Ruvo, Menotti; Cassani, Giovanni; Fassina, Giorgio

doi:10.1006/bbrc.1995.1014

Cited by 23 publications

(21 citation statements)

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“…The E6 and E7 proteins contained two and one conserved zinc-binding domains, CXXC(X) 29 CXXC (Ullman et al, 1996), respectively, separated by 36 aa. The E7 ORFs also contained a pRbbinding motif (LXCXE) (Radulescu et al, 1995). The ATPbinding site of the ATP-dependent helicase (GPPDTGKS) (Wilson et al, 2002) was conserved in the carboxylterminal region of E1 of HPV-110 and -111, whilst this binding site contained one mutation in HPV-107 and FA75[KI88-03] (GPPDSGKS; mutation indicated in bold).…”

Section: Characteristics Of the Novel Hpv Typesmentioning

confidence: 99%

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

Vasiljević

Hazard

Dillner

et al. 2008

Journal of General Virology

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Recent studies have suggested an association between human papillomaviruses (HPVs), particularly species 2 members of the genus Betapapillomavirus, and squamous cell carcinoma (SCC) of the skin. As most of these viruses are uncharacterized, molecular characterization and epidemiology are needed to advance our understanding of their significance in carcinogenesis. This study determined the complete genomes of four betapapillomaviruses of species 2 from skin lesions designated , an isolate of an unpublished HPV type, and analysed their prevalence and viral loads in biopsies from SCC, actinic keratosis (AK), basal cell carcinoma, seborrhoeic keratosis and the healthy skin of 263 immunocompetent patients by HPV type-specific real-time PCR assays. Seventeen patients (6.5 %) harboured at least one of the four HPV types in their lesion, whereas seven patients (2.7 %) harboured one or more of the HPV types in healthy skin. Overall, the four viruses were more common in AK than in healthy skin (odds ratio 5.0, 95 % confidence interval 1.4-17.5), but the prevalence and viral loads were low. This characterization of expands the heterogeneity of members of species 2 of the genus Betapapillomavirus. However, as these types were found in only a few samples and in low amounts, a possible role in carcinogenesis remains elusive.

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Section: Characteristics Of the Novel Hpv Typesmentioning

confidence: 99%

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

Vasiljević

Hazard

Dillner

et al. 2008

Journal of General Virology

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“…In another report [18], it was shown that multimerization of a peptide with binding properties for tumor necrosis factor (TNF ) synthesized using a heptalysine core, facilitates its immobilization on solid supports and provided multivalency to the interaction, ultimately allowing the purification of TNF fragments or even full-length recombinant TNF from a complex matrix such as crude Escherichia coli extracts. Similar approaches were followed by multimerizing ligands for -1 antitrypsin selected by screening phage display libraries [19], and binders for insulin deduced from the binding site on Retinoblastoma Protein [20].…”

Section: Multimeric Peptidesmentioning

confidence: 99%

“…The key advantageous concept for multimerization is that the bigger and the more flexible is the molecule, the higher will also be the available surface for interaction and also its avidity for the target protein [14,15,17,19,20]. Multimerization is generally achieved by straightforward approaches applicable to the solid phase method.…”

Section: Multimeric Peptidesmentioning

confidence: 99%

“…Incorporation of subsequent symmetrically protected lysines (or similar trifunctional residues), doubles at each step the available amino groups and by consequence the number of peptide copies that can be obtained on the multimer. display libraries, or designed ad hoc, which have been subsequently multimerized, generally in the form of tetramers, to improve affinity and stability [16,19,20,[24][25][26]. On the contrary, many other reports have described bioactive peptides in a multimeric format selected directly from libraries of multimerized tripeptides or more complex multimeric structures [9,22,23,[27][28][29].…”

Section: Multimeric Peptidesmentioning

confidence: 99%

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Branched Peptides for the Modulation of Protein-Protein Interactions: More Arms are Better than One?

Ruvo¹,

Sandomenico²,

Tudisco

et al. 2011

CMC

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Combinatorial peptide libraries from synthetic or biological sources have been largely used in the last two-decades with the aim of identifying bioactive peptides that specifically bind proteins and modulate their interactions with other protein partners. Differently from biological libraries, synthetic methods allow the development of different kinds of libraries based on two main characteristics: i) the use of building blocks and chemical bonds different from those naturally occurring and ii) the possibility of designing scaffolds with non-linear shapes, as cyclic and branched structures. These two features, alone or in combination, have increased the chemical and structural diversity of peptide libraries expanding the offer of collections for the screenings. Here we describe our and other experiences with branched peptides and the results obtained in the last fifteen years. These clearly indicate how the use of short multimerized peptides can represent a successful approach for different applications ranging from affinity chromatography to the modulation of protein-protein interactions in different biological contexts.

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“…From this initial insight, I extrapolated a more general model on intracellular hormone action and coined it "nucleocrine pathway" (8). Subsequently, the possibility of a physical interaction between insulin and RB -as the paradigm for nucleocrine signallingwas experimentally substantiated by several approaches (9)(10)(11)(12).…”

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confidence: 99%

Intracellular Insulin in Physiology and Disease: Past, Present and Future

Radulescu¹

2011

Acta Endo (Buc)

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A brief synopsis on the past three and a half decades of intracellular insulin research is given as it relates primarily to the human system. Thereby, a particular emphasis is placed on insulin binding and inactivation of the central tumor suppressor retinoblastoma protein (RB) and, moreover, on insulin degradation by the putative antioncoprotein insulin-degrading enzyme (IDE). Finally, the potential for (anticancer) therapeutics that target intracellular insulin is outlined.

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Binding of the LXCXE Insulin Motif to a Hexapeptide Derived from Retinoblastoma Protein

Cited by 23 publications

References 0 publications

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

Branched Peptides for the Modulation of Protein-Protein Interactions: More Arms are Better than One?

Intracellular Insulin in Physiology and Disease: Past, Present and Future

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