Binding of the cell adhesive protein tropoelastin to PTFE through plasma immersion ion implantation treatment

Bax, Daniel V.; Wang, Yiwei; Li, Zhe; Maitz, Peter; McKenzie, David R.; Bilek, Marcela M.M.; Weiss, Anthony S.

doi:10.1016/j.biomaterials.2011.03.079

Cited by 67 publications

(51 citation statements)

References 49 publications

(56 reference statements)

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“…The overlapping construct containing domains 17-27 (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27) confirmed that the major cell-binding site in N18 is in domains 17 and 18 of tropoelastin (Fig. 4A).…”

Section: Resultsmentioning

confidence: 68%

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A Novel Cell Adhesion Region in Tropoelastin Mediates Attachment to Integrin αVβ5

Lee

Bax

Bilek

et al. 2014

Journal of Biological Chemistry

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Background: Cellular integrin ␣ V ␤ 3 binds to RKRK at the C-terminal tail of tropoelastin. Results: Inhibition of integrin ␣ V ␤ 5 hinders cell adhesion to tropoelastin constructs comprising domains 17 and 18. Conclusion: Integrin ␣ V ␤ 5 mediates cell adhesion to tropoelastin through a central region. Significance: Understanding the cell adhesion activity of tropoelastin gives improved insight into the physiological and pathological cell responses to this protein.

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Section: Resultsmentioning

confidence: 68%

“…Cells (500 l) at a density of 2 ϫ 10 5 cells/ml in serum-free DMEM were added to each well and incubated for 1.5 h at 37°C. Cells were fixed with formaldehyde, and the actin cytoskeleton and nuclei were visualized as described (25).…”

Section: Methodsmentioning

confidence: 99%

A Novel Cell Adhesion Region in Tropoelastin Mediates Attachment to Integrin αVβ5

Lee

Bax

Bilek

et al. 2014

Journal of Biological Chemistry

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“…Kondyurin et al [16] used the same PIII treatment with PTFE 20 mm thick (Halogen, Perm, Russia) and found the water contact angle reduced from 1208 to 908. Bax et al [26] used PTFE 0.1 mm thick (Goodfellow) and observed a water contact angle change from 114.4 + 38 to 93 + 38. The resistance to change of the water contact angle of the PTFE used in our experiment is believed to be due to the presence of low-molecular-weight components in the material we are using (Goodfellow, 0.2 mm thick).…”

Section: Surface Characterizationmentioning

confidence: 99%

Ion-implanted polytetrafluoroethylene enhances Saccharomyces cerevisiae biofilm formation for improved immobilization

Tran

Kondyurin

Hirsh

et al. 2012

J. R. Soc. Interface.

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The surface of polytetrafluoroethylene (PTFE) was modified using plasma immersion ion implantation (PIII) with the aim of improving its ability to immobilize yeast. The density of immobilized cells on PIII-treated and -untreated PTFE was compared as a function of incubation time over 24 h. Rehydrated yeast cells attached to the PIII-treated PTFE surface more rapidly, with higher density, and greater attachment strength than on the untreated surface. The immobilized yeast cells were removed mechanically or chemically with sodium hydroxide and the residues left on the surfaces were analysed with Fourier transform infrared spectroscopy-attenuated total reflection (FTIR-ATR) and X-ray photoelectron spectroscopy (XPS). The results revealed that the mechanism of cell attachment on both surfaces differs and a model is presented for each. Rapid attachment on the PIII-treated surface occurs through covalent bonds of cell wall proteins and the radicals on the treated surface. In contrast, on the untreated surface, only physisorbed molecules were found in the residue and lipids were more highly concentrated than proteins. The presence of lipids in the residue was found to be a consequence of damage to the plasma membrane during the rehydration process and the increased cell stress was also apparent by the amount of Hsp12 in the protein residue. The immobilized yeast cells on PIII-treated PTFE were found to be as active as yeast cells in suspension.

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“…Fourier transform infrared (FTIR) and enzyme-linked immunosorbent assay (ELISA) measurements 49,50 were used to confirm that a monolayer of tropoelastin was covalently attached to the PEEK surfaces following PIII treatment (details are given in the supplementary material and Fig. S1 in the supplementary material).…”

Section: Resultsmentioning

confidence: 99%

Plasma ion implantation enabled bio-functionalization of PEEK improves osteoblastic activity

et al. 2018

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Slow appositional growth of bone in vivo is a major problem associated with polyether ether ketone (PEEK) based orthopaedic implants. Early stage promotion of osteoblast activity, particularly bone nodule formation, would help to improve contact between PEEK implantable materials and the surrounding bone tissue. To improve interactions with bone cells, we explored here the use of plasma immersion ion implantation (PIII) treatment of PEEK to covalently immobilize biomolecules to the surface. In this study, a single step process was used to covalently immobilize tropoelastin on the surface of PIII modified PEEK through reactions with radicals generated by the treatment. Improved bioactivity was observed using the human osteoblast-like cell line, SAOS-2. Cells on surfaces that were PIII-treated or tropoelastin-coated exhibited improved attachment, spreading, proliferation, and bone nodule formation compared to cells on untreated samples. Surfaces that were both PIII-treated and tropoelastin-coated triggered the most favorable osteoblast-like responses. Surface treatment or tropoelastin coating did not alter alkaline phosphatase gene expression and activity of bound cells but did influence the expression of other bone markers including osteocalcin, osteonectin, and collagen I. We conclude that the surface modification of PEEK improves osteoblast interactions, particularly with respect to bone apposition, and enhances the orthopedic utility of PEEK.

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Binding of the cell adhesive protein tropoelastin to PTFE through plasma immersion ion implantation treatment

Cited by 67 publications

References 49 publications

A Novel Cell Adhesion Region in Tropoelastin Mediates Attachment to Integrin αVβ5

A Novel Cell Adhesion Region in Tropoelastin Mediates Attachment to Integrin αVβ5

Ion-implanted polytetrafluoroethylene enhances Saccharomyces cerevisiae biofilm formation for improved immobilization

Plasma ion implantation enabled bio-functionalization of PEEK improves osteoblastic activity

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