Binding of Sheep Red Blood Cells to a Large Population of Human Lymphocytes

Lay, Waltraut H.; Mendes, Nelson F.; Bianco, Celso; Nussenzweig, Victor

doi:10.1038/230531a0

Cited by 413 publications

(110 citation statements)

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“…Furthermore, interactions between T and B cells during the proliferative response in vitro may not be recognized under such conditions. However, the distinctive property of human T cells to form rosettes with unsensitized sheep erythrocytes (SRBC) (23,24) offers a means to separate normal human peripheral blood T and B lymphocytes (25,26), and thus to approach this problem.…”

mentioning

confidence: 99%

Cellular Interactions in the Proliferative Response of Human T and B Lymphocytes to Phytomitogens and Allogeneic Lymphocytes

Lohrmann

Novikovs

Graw

1974

The Journal of Experimental Medicine

163

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The cellular events leading to proliferation of cultured human lymphocytes after in vitro stimulation with phytomitogens or allogeneic cells are poorly understood. Little is known on the nature of the proliferating lymphocytes, or whether interactions between different cell types are required for blastogenic transformation of the eventually responding lymphocytes. Investigating these questions, attention has to focus primarily on monocytes, and the different lymphocyte subpopulations.The role of the monocyte (or the monocyte-derived maerophage [1,2], respectively) in antigen-induced lymphocyte activation continues to be of great interest. Lymphocyte response to many antigens requires the presence of monocytes. Removal of glassadherent cells abolishes the in vitro antibody formation by mouse spleen cells against sheep erythrocytes (3) and antigen-induced transformation of human lymphocytes (4); addition of macrophages restores the lymphocyte reactivity (5, 6). Macrophagelymphocyte interaction is also required for lymphocyte activation by allogeneic histoincompatible lymphocytes in vitro, the mixed leukocyte culture (MLC) 1 (7-11). In contrast, lymphocyte activation by plant mitogens such as phytohemagglutinin (PHA), concanavalin A (Con A), or pokeweed mitogen (PWM) is widely attributed to direct interaction of the soluble mitogen with lymphocyte membrane receptors (12-15). Opinions about the role of monocytes in the mitogen-induced lymphocyte proliferation are controversial: reports that phagocytic cells inhibit the lymphocyte response to PHA (16) conflict with others that removal of phagocytic cells decreases lymphocyte activation by this mitogen (17, 18). Alter and Bach described potentiation of PHA-induced lymphocyte proliferation by monocytes (11), whereas Oppenheim and co-workers (4) observed this effect only at suboptimal doses of PHA.Lymphocytes can be separated into thymus-dependent (T) and thymus-independent (B) cells on the basis of ontogeny, function, and cell surface differentiation markers. Relatively small fractions of B or T cells from immunized animals will respond to the sensitizing antigens (19,20), whereas a large fraction of the lymphocyte inoculum will be stimulated in vitro by phytomitogens (21,22). Therefore, the lymphocyte response to phytomitogens is generally considered to be nonspecific, 1 Abbreviations used in this paper: Con A, concanavalin A; HBSS, Hanks' balanced salt solution; MLC, mixed leukocyte culture; N-SRBC, neuraminidase-pretreated sheep erythrocytes; PHA, phytohemagglutinin; PWM, pokeweed mitogen.

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confidence: 99%

Cellular Interactions in the Proliferative Response of Human T and B Lymphocytes to Phytomitogens and Allogeneic Lymphocytes

Lohrmann

Novikovs

Graw

1974

The Journal of Experimental Medicine

163

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“…T lymphoblasts are killed by deoxyadenosine or deoxyguanosine, rapidly accumulate dATP or dGTP, and have very low levels of 5'-nucleotidase (5-9). In contrast, B lymphoblasts grow normally in deoxyadenosine or deoxyguanosine, accumulate only small quantities of deoxynucleoside triphosphates, and have higher 5'-nucleotidase levels than T lymphoblasts (5-9 and four T lymphoblast lines (CEM, Jurkat, MSB-2, and MOLT-3) were maintained and characterized as described (10).Peripheral lymphocytes were obtained, separated into Erosette forming cells and non-E-rosette forming cells, and characterized by described methods (11)(12)(13). Lymphoid organs were obtained from children with no known immune dysfunction, and cells were separated from these tissues within 1 hr of surgery by using described methods (14) …”

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confidence: 99%

“…Peripheral lymphocytes were obtained, separated into Erosette forming cells and non-E-rosette forming cells, and characterized by described methods (11)(12)(13). Lymphoid organs were obtained from children with no known immune dysfunction, and cells were separated from these tissues within 1 hr of surgery by using described methods (14) …”

mentioning

confidence: 99%

Distribution of 5'-nucleotidase in human lymphoid tissues.

Edwards¹,

Gelfand²,

Burk³

et al. 1979

Proc. Natl. Acad. Sci. U.S.A.

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Low activity of 5'-nucleotidase (5'-ribonucleotide phosphohydrolase, EC 3.1.3.5) in T lymphoblasts may explain the marked sensitivity of this cell to deoxynucleotide accumulation when compared to B Iymphoblasts. The relevance of such observations with cultured cells to the normal immune system requires the demonstration of similar differences in the 5'-nucleotidase activity of normal human lymphocyte subpopulations. Sheep erythrocyte (E) rosette-forming cells from normal thymus, tonsi , and peripheral mononuclear cells have 5'-nucleotidase activities of 1.7, 11.3, and 21.2 nmol/hr per 106 cells. Non-E-rosette forming cells from the peripheral blood or tonsil have 5'-nucleotidase activity comparable to the higher levels found in the peripheral E-RFC. Increased levels of5'-nucleotidase activity may be a marker for post-thymic T Iymphocytes.T lymphoblasts have 5'-nucleotidase activity similar to values demonstrated for E-RFC in thymus, whereas cultured B lymphoblasts have 5'-nucleotidase activity 15 times greater than that of T lymphoblasts. On the basis of these observations, the 5'-nucleotidase deficiency in congenital agammaglobulinemia has been reevaluated. In these patients the data indicate that peripheral E-rosette forming cells have the enzyme deficiency, demonstrating an abnormality of T lymphocytes in this disorder of immunoglobulin production. Lymphocyte 5'-nucleotidase (5'-ribonucleotide phosphohydrolase, EC 3.1.3.5) may have an important role in the regulation of the human immune system. Decreased activity of this purine catabolic enzyme has been observed in patients with primary immunoglobulin deficiency (1-4). However, it remains unclear whether an etiological relationship exists between the immune dysfunction and this enzyme deficiency.A marked difference in 5'-nucleotidase activity has been proposed as the molecular basis for the differential susceptibility of B and T lymphoblast lines to the toxicity related to deoxynucleoside accumulation (5-7). T lymphoblasts are killed by deoxyadenosine or deoxyguanosine, rapidly accumulate dATP or dGTP, and have very low levels of 5'-nucleotidase (5-9). In contrast, B lymphoblasts grow normally in deoxyadenosine or deoxyguanosine, accumulate only small quantities of deoxynucleoside triphosphates, and have higher 5'-nucleotidase levels than T lymphoblasts (5-9 and four T lymphoblast lines (CEM, Jurkat, MSB-2, and MOLT-3) were maintained and characterized as described (10).Peripheral lymphocytes were obtained, separated into Erosette forming cells and non-E-rosette forming cells, and characterized by described methods (11)(12)(13). Lymphoid organs were obtained from children with no known immune dysfunction, and cells were separated from these tissues within 1 hr of surgery by using described methods (14)

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“…In addition, some patients were studied sequentially, to determine whether an association exists between Various assays were employed, including delayed hypersensitivity skin reaction (to recall antigens) peripheral-blood lymphocyte counts, formation of EAC sheep rosettes (a B-lymphocyte marker, (Bianco, Patrick and Nussenzweig, 1970)) E sheep rosettes, a T-lymphocyte marker, (Lay et al, 1971;Jondal, Holm and Wigzell, 1972) and a 51Cr-release assay of lymphocyte effector function. In the 5ICr-release assay the following parameters were studied: direct spontaneous cellular cytotoxicity, antibody-dependent cellular cvtotoxicity (MacLennan and Loewi, 1968;Perlmann and Holm, 1968), and PHA-stimulated cellular cytotoxicity, (Holm, Perlmann and Werner, 1964;Holm, and Perlmann 1967) against Chang target cells.…”

Section: Immunologicalmentioning

confidence: 99%

Lymphocyte function and response to chemo-immunotherapy in patients with metastatic melanoma

Thatcher¹,

Palmer²,

Gasiunas³

et al. 1977

Br J Cancer

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Summary.-Thirty-eight patients with metastatic melanoma were investigated for lymphocyte function immediately prior to chemo-immunotherapy. The pretreatment immune tests were compared with normal control values and with response to therapy. The "non-responder" group (but not "responder") had significantly reduced values for lymphocyte, null-cell and E-rosette-cell counts compared with controls. Lymphocytotoxicity (using a Chang target cell) showed the same pattern, with depression of direct and K-cell cytotoxic capacity in non-responders (statistically significant for direct cytotoxic capacity) but not in responders when compared with controls. Eight patients were studied sequentially whilst on treatment, and demonstrated considerable change (not statistically significant) in lymphocytotoxicity, an untreated "control" patient showed little variation. "Recall" -antigen skin testing showed no statistically significant difference between the patient groups.The data indicate that "non-T-cell activity" may be associated with response to chemo -immunotherapy.

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Binding of Sheep Red Blood Cells to a Large Population of Human Lymphocytes

Cited by 413 publications

References 4 publications

Cellular Interactions in the Proliferative Response of Human T and B Lymphocytes to Phytomitogens and Allogeneic Lymphocytes

Cellular Interactions in the Proliferative Response of Human T and B Lymphocytes to Phytomitogens and Allogeneic Lymphocytes

Distribution of 5'-nucleotidase in human lymphoid tissues.

Lymphocyte function and response to chemo-immunotherapy in patients with metastatic melanoma

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