Binding of positively charged particles to glutaraldehyde-fixed human erythrocytes

Weiss, Leonard; Zeigel, Robert F.; Jung, Okhee; Bross, Irwin D. J.

doi:10.1016/0014-4827(72)90181-4

Cited by 52 publications

(15 citation statements)

References 10 publications

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“…2c). This effect is often ascribed to the higher affinity of positively charged species to negatively charged phospholipids in the cellular membrane (Weiss et al 1972). Arvizo et al found this effect to cause depolarization of plasma membrane potential.…”

Section: Membrane Penetration Uptake Efficiency Biodistribution Andmentioning

confidence: 99%

Golden Perspective: Application of Laser‐Generated Gold Nanoparticle Conjugates in Reproductive Biology

Barchanski

Taylor

Klein

et al. 2011

Reprod Domestic Animals

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Contents The current demand for female calves has grown rapidly and controlling the sex of offspring provides an economically flexible management for the livestock producer. The only functioning method of efficiently producing separate populations of X and Y sperm in mammals is based on relative DNA differentiation by high‐speed flow cytometry. In this context, gold nanoparticles conjugated to sex chromosome‐specific moieties display promising application as novel fluorophor‐alternative for the high‐throughput screening, since they feature no photo bleaching, high quantum yield, good biocompatibility and the possibility of non‐destructive membrane penetration. Especially, gold nanoparticles fabricated by pulsed laser ablation are in the recent focus of interest, due to excellent biocompatibility, fabrication‐dependent, tuneable particle size as well as surface charge and ease of (bio)‐functionalization with a remarkably strong ligand binding. For the purpose of our studies functionalized gold nanoparticles may be used as novel markers for sex‐sorting of mammalian sperm and, depending on the selected probe, also for the selection of sperm with heritable DNA‐sequences interesting for animal breeding.

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Section: Membrane Penetration Uptake Efficiency Biodistribution Andmentioning

confidence: 99%

Golden Perspective: Application of Laser‐Generated Gold Nanoparticle Conjugates in Reproductive Biology

Barchanski

Taylor

Klein

et al. 2011

Reprod Domestic Animals

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“…2). However, 'as the distribution of negatively charged groups on mammalian cell surfaces does not appear to be homogeneous [12] one could envisage preferential contact of negatively charged liposomes with cell surface areas of lower than average surface charge [13]. It is also possible that the small size of liposomes could reduce the potential energy barrier opposing close contact with the cell surface [14,15].…”

Section: The Efiect Of the Surface Charge Of Liposomes On Their Elimimentioning

confidence: 99%

Control of the Rate of Hepatic Uptake and Catabolism of Liposome‐Entrapped Proteins Injected into Rats. Possible Therapeutic Applications

Gregoriadis

Neerunjun

1974

European Journal of Biochemistry

203

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I3'I-or '251-labelled albumin and B-fructofuranosidase were entrapped in liposomes composed of phosphatidylcholine, cholesterol and a charged lipid (molar ratio 7 : 2 : 1). Investigations on the fate of such liposomes injected into rats revealed the following.1. Initially there is an increased rate of removal from plasma (rapid phase) followed by a decreased one (slow phase). The rate of removal during the rapid phase was highest for negatively charged liposomes and lowest for positively charged liposomes with neutral liposomes in between. Uniform rates of removal, regardless of charge, were obtained during the slow phase.2. The rate of hepatic uptake of such liposomes paralleled that observed for their removal from plasma during the rapid phase.3. Concurrent administration of a large quantity of positively charged liposomes and of a smaller quantity of negatively charged liposomes led to a diminution of the rapid phase of the latter suggesting a common site of uptake for liposomes of either charge.4. Blockade of the reticuloendothelial system with carbon led to an increased hepatic localization of liposomes during the rapid phase suggesting a bimodal uptake of liposomes with both the Kupffer and the liver parenchymal cells involved.5. Cross-linking of liposome-entrapped albumin and B-fructofuranosidase increased the stability of the latter and following injection into rats rendered both proteins less vulnerable to degradation by the liver lysosomal enzymes.6. The possibility of controlling both the rate of removal from plasma and catabolism of liposomeentrapped proteins adds more scope to the use of liposomes in therapy.

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“…Differences have been 218 ThE JOURNAL OF CELL BIOLOGY 9 VOLUME 71, 1976 9 pages 218-231 found between various erythroid developmental stages in surface density of negative charges (34,36), cell surface antigenicity (22,38), and pyroantimonate (1) and Con A (2) binding. By using positively charged colloidal ferric oxide (which binds to sialic acid residues [12,20,42]) it was previously shown that the density of anionic groups in developing rabbit erythrocytes gradually decreases with successive divisions of the proerythroblasts, then reincreases after expulsion of the nucleus and on maturation of the reticulocyte to become a mature erythrocyte (13,15,36). If one assumes that the binding sites are associated with glycoproteins, these modulations in the surface charge density suggest that the rate of synthesis and insertion into the red blood cell membrane of different plasma membrane proteins varies at specific stages in the differentiation process.…”

mentioning

confidence: 99%

Variations in distribution of con A receptor sites and anionic groups during red blood cell differentiation in the rat.

Skutelsky¹,

Farquhar²

1976

The Journal of Cell Biology

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The distribution of receptors for concanavalin A (Con A) and anionic groups on plasma membranes of developing blood cells was investigated in the rat. Glutaraldehyde-fixed bone marrow and circulating blood cells were exposed to ferritinconjugated Con A or positively charged ferric oxide (CI) and processed for electron microscopy. The frequency of Con A and CI binding sites varied during different erythroid developmental stages and among different Jeukoid cell types. There was a constant inverse relationship between Con A and CI binding sites. Among leukoid cells, Con A binding was high on plasma cells and macrophages, lower on neutrophils and lymphocytes, and still lower on eosinophils and basophils; CI binding was highest in the latter and lowest in plasma cells and macrophages. Among erythroid cells, there was a progressive increase in Con A and a decrease in CI binding after successive divisions of erythroblasts, and a progressive decrease in Con A and an increase in CI binding upon maturation of the orthochromatic erythroblast to the reticulocyte. The most pronounced modification in distribution of these sites occurred during nuclear expulsion: that portion of the plasma membrane surrounding the extruded nucleus was heavily labeled by Con A (up to four times that of the orthochromatic erythroblast) whereas the reticulocyte had considerably fewer sites. The situation was reversed with CI. The results suggest that the concentration of nonsialated glycoproteins (to which Con A binds) varies inversely to that of sialoproteins (to which CI binds) in the membrane of the differentiating erythroid cell. The remarkable changes observed at the time of nuclear extrusion suggest that there is either local modification of glycosylated groups with removal of sialyl residues from the membrane surrounding the extruded nucleus or selective segregation of membrane glycoproteins leading to a high concentration of sialoproteins (glycophorin) in the membrane of the mature erythrocyte.Previous studies utilizing electron microscope labeling techniques have established that differentiation of the mammalian erythrocyte is associated with significant changes in the density and distribution of binding sites (presumably glycoproteins) on the plasma membrane. Differences have been

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Binding of positively charged particles to glutaraldehyde-fixed human erythrocytes

Cited by 52 publications

References 10 publications

Golden Perspective: Application of Laser‐Generated Gold Nanoparticle Conjugates in Reproductive Biology

Golden Perspective: Application of Laser‐Generated Gold Nanoparticle Conjugates in Reproductive Biology

Control of the Rate of Hepatic Uptake and Catabolism of Liposome‐Entrapped Proteins Injected into Rats. Possible Therapeutic Applications

Variations in distribution of con A receptor sites and anionic groups during red blood cell differentiation in the rat.

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