Binding of PFOS to serum albumin and DNA: insight into the molecular toxicity of perfluorochemicals

Zhang, Xian; Ling, Chen; Fei, Xunchang; Ma, Ying-jeou; Gao, Hong‐Wen

doi:10.1186/1471-2199-10-16

Cited by 90 publications

(65 citation statements)

References 80 publications

(73 reference statements)

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“…Ce 3+ can insert into DNA base pairs and bind to nucleotide. Our results are also consistent with the previous studies on the effects of other heavy metal ions on DNA [18][19][20][21].…”

Section: Uv-vis Absorption Spectra Of Dna From the Mouse Liversupporting

confidence: 95%

The Impairment of Liver DNA Conformation and Liver Apoptosis of Mice Caused by CeCl3

Cheng

Cai

et al. 2010

Biol Trace Elem Res

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Cerium (Ce) was shown to cause various toxic effects both in rats and mice; however, the molecular mechanism by which Ce exert theirs toxicity is still understood. In this report, the impairment of liver DNA conformation and liver apoptosis of mice caused by CeCl(3) was studied in vivo using inductively coupled plasma-mass spectrometry, various spectral methods, gel electrophoresis, and transmission electron micrograph. We found that the coefficients of liver to body weight of the mice treated with CeCl(3) were significantly increased. Ce(3+) could be significantly accumulated in the liver, and it insert itself into DNA base pairs and/or bind to DNA nucleotide, and alter the conformation of DNA. Furthermore, the evaluation by gel electrophoresis and transmission electron micrograph showed that higher dose of Ce(3+) could cause DNA cleavage and hepatocyte apoptosis in mice. Therefore, our study aroused the attention of Ce application and exposure effects especially on human liver for long-term and low-dose treatment.

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“…Ce 3+ can insert into DNA base pairs and bind to nucleotide. Our results are also consistent with the previous studies on the effects of other heavy metal ions on DNA [18][19][20][21].…”

Section: Uv-vis Absorption Spectra Of Dna From the Mouse Liversupporting

confidence: 95%

The Impairment of Liver DNA Conformation and Liver Apoptosis of Mice Caused by CeCl3

Cheng

Cai

et al. 2010

Biol Trace Elem Res

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“…26,27) It is common in bioanalysis to add an acid (o en phosphoric acid) to release compounds associated with proteins.…”

Section: −1mentioning

confidence: 99%

Sensitive Detection of Aromatic Hydrophobic Compounds in Water and Perfluorooctane Sulfonate in Human Serum by Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) with Amine Functionalized Graphene-Coated Cobalt Nanoparticles

et al. 2014

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In this article, we describe the application of surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) with the use of amine functionalized graphene-coated cobalt nanoparticles (CoC-NH 2 nanoparticles) to analyse aromatic hydrophobic compounds that are known environmental contaminants, including polycyclic aromatic hydrocarbons (PAHs) and pentachlorophenol (PCP). Our results demonstrated that SALDI-MS can detect PCP, anthracene, and pyrene in water. In particular, the CoC-NH 2 nanoparticles proved to be an e cient means of capturing PCP in water because of the high adsorption capacity of the nanoparticles for PCP, which resulted in a detectability of 100 ppt. Furthermore, the CoC-NH 2 nanoparticles also functioned as an adsorbent for solid-phase extraction of per uorooctane sulfonate (PFOS) from human serum, displaying good performance with a detectability of 10 ppb by SALDI-MS.

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“…22 The ratio of PFOS to HSA at saturation binding was determined to be 45:1. 23 Molecular modeling was also used to study the binding sites of PFOS on HSA, which showed that the tight binding site is near Trp214 or Sudlow site I and that the maximum number of PFOS binding to HSA is 11. 24 However, the structural details of PFOS binding to HSA remain unclear.…”

Section: * S Supporting Informationmentioning

confidence: 99%

“…The transformation temperature of the HSA−PFOS complex is 4°C higher than that of HSA (Supporting Information, Figure S3), indicating that the PFOS binding increases albumin thermal stability. Previous studies using isothermal titration calorimetry showed that the interactions of PFOS with HSA were spontaneous and driven by entropy change, 23 which indicated that the HSA−PFOS complex becomes less mobile compared to HSA without ligands.…”

Section: * S Supporting Informationmentioning

confidence: 99%

Structural Evidence of Perfluorooctane Sulfonate Transport by Human Serum Albumin

Luo

Shi

et al. 2012

Chem. Res. Toxicol.

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Perfluorooctane sulfonate (PFOS) is a man-made fluorosurfactant and globally persistent organic pollutant. PFOS is mainly distributed in blood with a long half-life for elimination. PFOS was found mainly bound to human serum albumin (HSA) in plasma, the most abundant protein in human blood plasma, which transports a variety of endogenous and exogenous ligands. However, the structural basis of such binding remains unclear. Here, we report the crystal structure of the HSA−PFOS complex and show that PFOS binds to HSA at a molar ratio of 2:1. In addition, PFOS binding renders the HSA structure more compact. Our results provide a structural mechanism to understand the retention of surfactants in human serum. P erfluorooctane sulfonate (PFOS) (Supporting Information, Figure S1) is a stable chemical with high surface activity, high thermal and acid resistance, and with both hydroand lipophobic properties. PFOS have been used in a wide range of industrial and commercial applications since 1950s.

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Binding of PFOS to serum albumin and DNA: insight into the molecular toxicity of perfluorochemicals

Cited by 90 publications

References 80 publications

The Impairment of Liver DNA Conformation and Liver Apoptosis of Mice Caused by CeCl3

The Impairment of Liver DNA Conformation and Liver Apoptosis of Mice Caused by CeCl3

Sensitive Detection of Aromatic Hydrophobic Compounds in Water and Perfluorooctane Sulfonate in Human Serum by Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) with Amine Functionalized Graphene-Coated Cobalt Nanoparticles

Structural Evidence of Perfluorooctane Sulfonate Transport by Human Serum Albumin

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