2001
DOI: 10.1034/j.1399-302x.2001.016002088.x
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Binding of oral streptococci to human fibrinogen

Abstract: The interaction of oral streptococci with human fibrinogen was investigated. Streptococcus gordonii was chosen as a representative species to study the binding to fibrinogen. S. gordonii DL1 adhered to immobilized fibrinogen and bovine serum albumin. Binding to immobilized fibrinogen was saturable, concentration and temperature dependent. The binding of S. gordonii DL1 to fibrinogen was inhibited by anti-fibrinogen antibody. Heating of the bacteria for 1 h at 95 degrees C resulted in 90% inhibition of the bind… Show more

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Cited by 15 publications
(12 citation statements)
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“…In contrast, S. oralis , which is as common as S. sanguinis (Douglas et al , 1993) in infective endocarditis, has no direct affinity for platelets, and so we speculate that this organism colonizes vegetations by means of recognition of one or more matrix proteins present (e.g. fibrin, fibronectin; Lee et al , 2001). Of course, such binding may also contribute to S. gordonii and S. sanguinis colonization.…”
Section: Discussionmentioning
confidence: 90%
“…In contrast, S. oralis , which is as common as S. sanguinis (Douglas et al , 1993) in infective endocarditis, has no direct affinity for platelets, and so we speculate that this organism colonizes vegetations by means of recognition of one or more matrix proteins present (e.g. fibrin, fibronectin; Lee et al , 2001). Of course, such binding may also contribute to S. gordonii and S. sanguinis colonization.…”
Section: Discussionmentioning
confidence: 90%
“…For example, Group A and G streptococci can bind both soluble and immobilized forms of fibrinogen, whereas several oral streptococci appear to bind only immobilized fibrinogen [27], [28], [29]. To assess whether fibrinogen binding by lysin SM1 was phase-dependent, we reversed the binding conditions, such that FLAG lysin SM1 , untagged lysin SM1 , and FLAG AP (all at 10 µg/ml) were immobilized in microtiter wells, and probed with the increasing concentration of fibrinogen in solution.…”
Section: Resultsmentioning
confidence: 99%
“…Subsequent opportunistic introduction of the bacteria through the circulation allows dissemination to extra-oral sites via interaction of these streptococci with extracellular matrix molecules or blood components which function as cell receptors for these bacteria [1][2][3][4][5]. Subsequent opportunistic introduction of the bacteria through the circulation allows dissemination to extra-oral sites via interaction of these streptococci with extracellular matrix molecules or blood components which function as cell receptors for these bacteria [1][2][3][4][5].…”
Section: Introductionmentioning
confidence: 99%