Binding of metals to cell envelopes of Escherichia coli K-12

Beveridge, Terry J.; Koval, Susan F.

doi:10.1128/aem.42.2.325-335.1981

Cited by 218 publications

(76 citation statements)

References 68 publications

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“…2) can be attributed to sorption of Fe (precipitation or adsorption) on the PG. This interpretation is in accordance with indirect observations of the capacity of the PG of E. coli to bind different metals (Beveridge & Koval, 1981). Positively charged iron precipitates could establish bonds with the anionic carboxyl groups of the D-glutamic acid residues of the PG.…”

Section: Cemovis Analyses Reveal Fine Ultrastructural Details Of Cellsupporting

confidence: 90%

Preservation of protein globules and peptidoglycan in the mineralized cell wall of nitrate-reducing, iron(II)-oxidizing bacteria: a cryo-electron microscopy study

et al. 2011

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Iron-oxidizing bacteria are important actors of the geochemical cycle of iron in modern environments and may have played a key role all over Earth's history. However, in order to better assess that role on the modern and the past Earth, there is a need for better understanding the mechanisms of bacterial iron oxidation and for defining potential biosignatures to be looked for in the geologic record. In this study, we investigated experimentally and at the nanometre scale the mineralization of iron-oxidizing bacteria with a combination of synchrotron-based scanning transmission X-ray microscopy (STXM), scanning transmission electron microscopy (STEM) and cryo-transmission electron microscopy (cryo-TEM). We show that the use of cryo-TEM instead of conventional microscopy provides detailed information of the successive iron biomineralization stages in anaerobic nitrate-reducing iron-oxidizing bacteria. These results suggest the existence of preferential Fe-binding and Fe-oxidizing sites on the outer face of the plasma membrane leading to the nucleation and growth of Fe minerals within the periplasm of these cells that eventually become completely encrusted. In contrast, the septa of dividing cells remain nonmineralized. In addition, the use of cryo-TEM offers a detailed view of the exceptional preservation of protein globules and the peptidoglycan within the Fe-mineralized cell walls of these bacteria. These organic molecules and ultrastructural details might be protected from further degradation by entrapment in the mineral matrix down to the nanometre scale. This is discussed in the light of previous studies on the properties of Fe-organic interactions and more generally on the fossilization of mineral-organic assemblies.

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Section: Cemovis Analyses Reveal Fine Ultrastructural Details Of Cellsupporting

confidence: 90%

Preservation of protein globules and peptidoglycan in the mineralized cell wall of nitrate-reducing, iron(II)-oxidizing bacteria: a cryo-electron microscopy study

et al. 2011

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“…In long-term bioassays with sublethal endpoints such as development or reproduction, the test organisms have to be fed during the exposure. From the literature it is known that E. coli can bind heavy metals [56,57], which can lead to a decrease in the effective concentration of the toxicant in the test medium. On the other hand, Cd bound by E. coli can enter the worms through the gut, so a decrease in the concentration of free Cd 2ϩ may be compensated for by the uptake of bacteria containing Cd.…”

Section: Discussionmentioning

confidence: 99%

Ecotoxicological Assessment of Aquatic Sediments With Caenorhabditis Elegans (Nematoda)—a Method for Testing Liquid Medium and Whole-Sediment Samples

Traunspurger¹,

Haitzer²,

Höss³

et al. 1997

Environ Toxicol Chem

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Abstract-We present a method using the free-living nematode Caenorhabditis elegans (Maupas, 1899) to assess toxicity in liquid medium and whole-sediment setups. Test duration is 72 h; endpoints are body length, number of eggs inside worms, percentage of gravid worms, and number of offspring per worm. The effect of CdCl 2 on C. elegans in liquid-phase exposures is described as an example. Results from a field study with polluted sediments from the River Elbe (Germany) suggest that nematodes may be useful organisms in assessing toxicity of sediments in the whole phase.

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“…Some bacterial cell surfaces are highly reactive towards binding of metal ions (Beveridge, 1989) and preformed nanominerals (Glasauer et al ., 2001). In Gram negative bacteria, this reactivity arises from the presence of a wide array of ionizable groups, such as carboxylates and phosphates, present in the lipopolysaccharide matrix of the cell wall as demonstrated for Escherichia coli (Beveridge and Koval, 1981). In the case of catalysts like palladium, small nanoparticles represent a large specific surface area and this has large implications towards electron cloud distribution and, concomitantly, reactivity of the catalyst.…”

Section: Introductionmentioning

confidence: 99%

Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls

Windt

Aelterman

Verstraete

2005

Environmental Microbiology

337

196

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Microbial reduction of soluble Pd(II) by cells of Shewanella oneidensis MR-1 and of an autoaggregating mutant (COAG) resulted in precipitation of palladium Pd(0) nanoparticles on the cell wall and inside the periplasmic space (bioPd). As a result of biosorption and subsequent bioreduction of Pd(II) with H2, formate, lactate, pyruvate or ethanol as electron donors, recoveries higher than 90% of Pd associated with biomass could be obtained. The bioPd(0) nanoparticles thus obtained had the ability to reductively dehalogenate polychlorinated biphenyl (PCB) congeners in aqueous and sediment matrices. Bioreduction was observed in assays with concentrations up to 1000 mg Pd(II) l(-1) with depletion of soluble Pd(II) of 77.4% and higher. More than 90% decrease of PCB 21 (2,3,4-chloro biphenyl) coupled to formation of its dechlorination products PCB 5 (2,3-chloro biphenyl) and PCB 1 (2-chloro biphenyl) was obtained at a concentration of 1 mg l(-1) within 5 h at 28 degrees C. Bioreductive precipitation of bioPd by S. oneidensis cells mixed with sediment samples contaminated with a mixture of PCB congeners, resulted in dechlorination of both highly and lightly chlorinated PCB congeners adsorbed to the contaminated sediment matrix within 48 h at 28 degrees C. Fifty milligrams per litre of bioPd resulted in a catalytic activity that was comparable to 500 mg l(-1) commercial Pd(0) powder. The high reactivity of 50 mg l(-1) bioPd in the soil suspension was reflected in the reduction of the sum of seven most toxic PCBs to 27% of their initial concentration.

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Binding of metals to cell envelopes of Escherichia coli K-12

Cited by 218 publications

References 68 publications

Preservation of protein globules and peptidoglycan in the mineralized cell wall of nitrate-reducing, iron(II)-oxidizing bacteria: a cryo-electron microscopy study

Preservation of protein globules and peptidoglycan in the mineralized cell wall of nitrate-reducing, iron(II)-oxidizing bacteria: a cryo-electron microscopy study

Ecotoxicological Assessment of Aquatic Sediments With Caenorhabditis Elegans (Nematoda)—a Method for Testing Liquid Medium and Whole-Sediment Samples

Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls

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