Binding of Metal Ions by Extracellular Polymers of Biofilm Bacteria

Geesey, Gill G.; Jang, Larry K.; Jolley, J.G.; Hankins, M.R.; Iwaoka, Teiki; Griffiths, Peter R.

doi:10.2166/wst.1988.0279

Cited by 92 publications

(47 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These may include ionic groups and hydrophobic residues. The presence of ionic groups such as carboxyl, phosphoyl and sulfonyl potentially allow the complexation of cations including metals (Geesey et al 1989). Some amino sugars and proteins are also cationic and may provide sites for binding of anionic compounds (Decho 1990).…”

Section: Introductionmentioning

confidence: 99%

Effect of CNP on composition and structure of lotic biofilms as detected with lectin-specific glycoconjugates

Neu¹,

Swerhone²,

Böckelmann³

et al. 2005

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

Biofilms were cultivated in rotating annular reactors supplied with river water supplemented with carbon, nitrogen, phosphorus or a combined nutrient addition. Confocal laser scanning microscopy and digital image analysis were used in combination with a panel of fluor-conjugated lectin probes to assess the changes in biofilm glycoconjugates with depth, time and in response to the nutrient additions. In addition, nucleic acid staining and autofluorescence were utilised to monitor bacterial and photosynthetic populations respectively. These analyses indicated that in lotic biofilms both quantitative and proportional differences in glycoconjugate composition developed under each nutrient regime. The differences in the biofilms were detected with time and with depth. Comparison of C, N, P, and CNP treatments with the control showed differences in the total amount of photosynthetic organisms, bacteria and extracellular polymeric substance (EPS)-specific glycoconjugates. For example, C, N, and CNP nutrient additions resulted in a minimal increase of 50% lectin-specific EPS glycoconjugates. Accumulation of different glycoconjugates over time in the different biofilms showed a similar pattern in the single nutrient treatments, with a maximum accumulation at 3 wk, followed by sloughing in Week 5 and regrowth in Week 7. In contrast, both the control and the combined CNP treatment showed a continuous increase and a plateau phase for the glycoconjugates as a whole. Both visualisation and quantification indicated that under each treatment the distribution of 4 specific glycoconjugates (Arachis hypogaea, Canavalia ensiformis, Glycine max, Ulex europaeus) varied with depth and, in addition, changed over time. Further, the glycoconjugate make-up of the single nutrient additions were similar to each other, as was the make-up of the control and combined nutrient treatment. Application of fluorescence in situ hybridisation analysis indicated that significant changes occurred in the beta-proteobacteria community composition as a consequence of nutrient addition. The results demonstrate the effect of nutrient regime on the cellular composition and glycoconjugate chemistry of lotic biofilms.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of CNP on composition and structure of lotic biofilms as detected with lectin-specific glycoconjugates

Neu¹,

Swerhone²,

Böckelmann³

et al. 2005

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

show abstract

“…The chemical reactions between microorganisms and metals have been reviewed by Ford and Mitchell (4) and include: a) intracellular accumulation of metals (5,6); b) association of metals with the cell wall (7); c) metal-siderophore interactions (8); d) extracellular mobilization/immobilization of metals by bacterial metabolic products (9,10); e) extracellular polymer-metal interactions (11)(12)(13); and f) transformation and volatization of metals (14). The applications of processes a, b, and f involve the use of whole bacterial cells, and, therefore, may be difficult to maintain and operate in soils (15 (23,25).…”

Section: Introductionmentioning

confidence: 99%

Trace metal mobilization in soil by bacterial polymers.

Chen

Czajka

Lion

et al. 1995

Environ Health Perspect

View full text Add to dashboard Cite

Enhanced transport of trace metal in porous media can occur in the presence of a ligand or "carrier" that has a high affinity for binding the pollutant, is dispersed and mobile in the soil environment, is recalcitrant with respect to microbial degradation, and is acceptable to the public. These aspects of the facilitated transport to trace metals are discussed with respect to a naturally occurring carrier: extracellular polymers of bacterial origin. The literature is reviewed regarding the production and composition of bacterial extracellular polymers, the processes relevant to the facilitated transport of trace metals in soil by bacterial polymers, and potential for transformation of polymers in soils by microbial degradation. Model calculations of contaminant retardation are presented for the case of polymer-mediated transport of cadmium in a sandy aquifer material. The available information suggests that extracellular polymers can bind metal ions and are mobile in the soil environment. Extracellular polymers also appear to be relatively slowly degraded by soil microorganisms. These properties and the supporting model calculations indicate that extracellular polymers of bacterial origin merit consideration as agents that may be applied to contaminated soils to enhance trace metal mobility. -Environ Health Perspect 1 03(Suppl 1):53-58 (1995)

show abstract

“…One of the important properties of EPS is their ability to complex with metal ions. The reason for the complex is that macromolecules in the biofilms contain anionic functional groups such as carboxyl, phosphate, sulfate, glycerate and pyruvate groups which are involved in the interaction with metal ions [24,25]. The capacity of EPS to complex metal ions depends both on bacterial species and on the type of metal ion [26,27].…”

Section: Biofilm Chemical Analysismentioning

confidence: 99%