Binding of kidney bean (Phaseolus vulgaris) isolectins to differentiated human colon carcinoma Caco-2 cells and their effect on cellular metabolism.

Abstract

The binding of Phaseolus vulgaris (PHA) isolectins L4 and E4 Both isolectins stimulated the incorporation of thymidine and glucosamine, but neither PHA-L4 nor PHA-E4 were able to influence the incorporation of uridine. With respect to fucose, methionine, and N-acetyl mannosamine, the stimulatory effect remained confined to PHA-E4. Since PHA-L4 and PHA-E4 were tested at the same concentrations, PHA-E4 is more effective than PHA-L4. The changes in the uptake of radioactive precursors were lost after heat inactivation of PHA-E4. Compared with control and PHA-L4 incubated Caco-2 cells, the microvilli of PHA-E4 incubated cells were shortened significantly (p<0-01).

Sucrose Co-administration Reduces the Toxic Effect of Lectin on Gut Permeability and Intestinal Bacterial Colonization

“…This is thought to be due to malabsorption of nutrients since distortion of intestinal architecture and loss of brush border enzymes [11,16,20,21] have been seen. These reports followed a relatively long duration of exposure in weeks or months.…”

Mechanisms of Lectin (Phytohemagglutinin)-Induced Growth in Small Intestinal Epithelial Cells

“…Previous studies revealed that the growth-promoting effects of PHA resulted from specific binding to carbohydrates on the cell surface [10]. N-acetyl- D -galactosamine had been shown to specifically inhibit hemagglutination and leukoagglutination by PHA without destroying either the cells or the PHA [35].…”

“…In addition, lectins stimulate proliferation in the large intestine and the pancreas [7, 8]. At the cellular level, binding and endocytosis of PHA by epithelial cells increased DNA and RNA contents [9, 10]and stimulated cellular protein synthesis [11], but the underlying mechanisms are unknown.…”

Flagella-mediated bacterial motility accelerates but is not required for Salmonella serotype Enteritidis invasion of differentiated Caco-2 cells

“…A multiplicity of infection (moi) of 100-400 bacteria per Caco-2 cell was used. Caco-2 cells were routinely grown as described previously (Hendriks et al, 1991).…”