Binding of <i>Trypanosoma congolense</i> to the Walls of Small Blood Vessels*

Banks, Keith L.

doi:10.1111/j.1550-7408.1978.tb04405.x

Cited by 59 publications

(35 citation statements)

References 20 publications

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“…These findings are consistent with the well-known phenotypic variation between endothelial cells from different species and, moreover, from different locations within the same species [7]. Interestingly, the tissue specificity observed with T. congolense activation is consistent with the tropism to lung, bone marrow and peripheral lymph nodes observed in vivo [5]. Nevertheless, the observed species-specific activation of endothelial cells by T. congolense remains to be elucidated.…”

Section: Discussionsupporting

confidence: 87%

“…At a certain stage of infection, T. b. gambiense and T. vivax invade internal organs, including the central nervous system, which requires direct contact with the endothelial cells of blood brain barrier (BBB) [3], [4]. On the contrary, T. congolense remains exclusively intravascular, but binds to the walls of capillaries of infected cattle and to bovine aortic endothelial cells (BAE) in vitro [5], [6].…”

Section: Introductionmentioning

confidence: 99%

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Identification of Trans-Sialidases as a Common Mediator of Endothelial Cell Activation by African Trypanosomes

et al. 2013

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Understanding African Trypanosomiasis (AT) host-pathogen interaction is the key to an “anti-disease vaccine”, a novel strategy to control AT. Here we provide a better insight into this poorly described interaction by characterizing the activation of a panel of endothelial cells by bloodstream forms of four African trypanosome species, known to interact with host endothelium. T. congolense, T. vivax, and T. b. gambiense activated the endothelial NF-κB pathway, but interestingly, not T. b. brucei. The parasitic TS (trans-sialidases) mediated this NF-κB activation, remarkably via their lectin-like domain and induced production of pro-inflammatory molecules not only in vitro but also in vivo, suggesting a considerable impact on pathogenesis. For the first time, TS activity was identified in T. b. gambiense BSF which distinguishes it from the subspecies T. b. brucei. The corresponding TS were characterized and shown to activate endothelial cells, suggesting that TS represent a common mediator of endothelium activation among trypanosome species with divergent physiopathologies.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Identification of Trans-Sialidases as a Common Mediator of Endothelial Cell Activation by African Trypanosomes

et al. 2013

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“…However, this organelle appears to play a key role in the cell adhesion events which occur at different lifecycle stages within the insect vector and in animal hosts. In infected animals, T. congolense bloodstream forms are found adhering to erythrocytes and to endothelial cells of the microvasculature through their flagellum (Banks, 1978(Banks, , 1979(Banks, , 1980. The direct effects of this process are not entirely understood, but the tissue surrounding the adhesion sites is damaged by release of a vascular permeabilization factor, and the subsequent action of the host's immune system (Banks, 1980).…”

Section: Introductionmentioning

confidence: 99%

The interaction ofTrypanosoma congolensewith endothelial cells

1994

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SUMMARYFactors which affect adhesion of cultured Trypanosoma congolense bloodstream forms to mammalian feeder cells have been examined. Using an in vitro binding assay, the initial events following interaction of trypanosomes with bovine aorta endothelial (BAE) cells were monitored by both light-and electron microscopy. Metabolic inhibitors and other biochemicals were incubated with either cells or parasites, to test whether any inhibited the process. Our findings suggest that adhesion of the parasites is an active process requiring metabolic energy from the trypanosomes, but not from endothelial cells. We also provide data suggesting that T. congolense bloodstream forms possess a lectin-like domain, localized at distinct sites on their flagellar surface, which interacts with specific carbohydrate receptors, most likely sialic acid residues, on the endothelial cell plasma membrane. We also suggest that the cytoskeletal protein actin is probably involved in this interaction.

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“…This could be explained by the unique life cycles of these two species. In contrast to T. brucei , T. congolense is a strictly intravascular parasite and does not traverse different tissues71. T. equiperdum is essentially a tissue parasite in the reproductive system of Equidae family animals with a very low parasitemia in the blood and is transmitted venereally72.…”

Section: Discussionmentioning

confidence: 99%

Two flagellar BAR domain proteins in Trypanosoma brucei with stage-specific regulation

Čičová

Dejung

Skalický

et al. 2016

Sci Rep

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Trypanosomes are masters of adaptation to different host environments during their complex life cycle. Large-scale proteomic approaches provide information on changes at the cellular level, and in a systematic way. However, detailed work on single components is necessary to understand the adaptation mechanisms on a molecular level. Here, we have performed a detailed characterization of a bloodstream form (BSF) stage-specific putative flagellar host adaptation factor Tb927.11.2400, identified previously in a SILAC-based comparative proteome study. Tb927.11.2400 shares 38% amino acid identity with TbFlabarin (Tb927.11.2410), a procyclic form (PCF) stage-specific flagellar BAR domain protein. We named Tb927.11.2400 TbFlabarin-like (TbFlabarinL), and demonstrate that it originates from a gene duplication event, which occurred in the African trypanosomes. TbFlabarinL is not essential for the growth of the parasites under cell culture conditions and it is dispensable for developmental differentiation from BSF to the PCF in vitro. We generated TbFlabarinL-specific antibodies, and showed that it localizes in the flagellum. Co-immunoprecipitation experiments together with a biochemical cell fractionation suggest a dual association of TbFlabarinL with the flagellar membrane and the components of the paraflagellar rod.

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Binding of Trypanosoma congolense to the Walls of Small Blood Vessels*

Cited by 59 publications

References 20 publications

Identification of Trans-Sialidases as a Common Mediator of Endothelial Cell Activation by African Trypanosomes

Identification of Trans-Sialidases as a Common Mediator of Endothelial Cell Activation by African Trypanosomes

The interaction ofTrypanosoma congolensewith endothelial cells

Two flagellar BAR domain proteins in Trypanosoma brucei with stage-specific regulation

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