Binding of Hg2+ by Cys, Cys-Gly and reduced glutathione: Study by differential pulse voltammetry on rotating Au-disk electrode, electrospray ionization mass-spectrometry and isothermal titration calorimetry

Chekmeneva, Elena; Dı́az-Cruz, José Manuel; Ariño, Cristina; Esteban, Miquel

doi:10.1016/j.jelechem.2010.03.021

Cited by 22 publications

(8 citation statements)

References 30 publications

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“…Previous studies have reported the use of ESI with lower-resolution MS to investigate interactions between Hg and simple peptides such as cysteine and glutathione. − It was demonstrated that ESI-MS can detect Hg-containing mass signals directly. , Coupled with distinct Hg isotope distribution patterns, characteristic mass peaks of Hg–amino acid or Hg–peptide complexes were identified, mostly under positive ionization mode. , The present study was undertaken to explore the feasibility of using FTICR-MS to directly measure and identify specific Hg–DOM complexes in heterogeneous DOM samples. Stable Hg isotope distribution patterns were used to confirm the Hg–DOM mass peaks and their assigned molecular formulas with a resolution of 400,000 (at m / z 400) and an accuracy of <1 ppm.…”

Section: Introductionmentioning

confidence: 96%

Identification of Mercury and Dissolved Organic Matter Complexes Using Ultrahigh Resolution Mass Spectrometry

Chen

Johnston

Mann

et al. 2016

Environ. Sci. Technol. Lett.

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The chemical speciation and bioavailability of mercury (Hg) is markedly influenced by its complexation with naturally dissolved organic matter (DOM) in aquatic environments. To date, however, analytical methodologies capable of identifying such complexes are scarce. Here, we utilize ultrahigh resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) coupled with electrospray ionization to identify individual Hg–DOM complexes. The measurements were performed by direct infusion of DOM in a 1:1 methanol:water solution at a Hg to dissolved organic carbon (DOC) molar ratio of 3 × 10–4. Heteroatomic molecules, especially those containing multiple S and N atoms, were found to be among the most important in forming strong complexes with Hg. Major Hg–DOM complexes of C10H21N2S4Hg+ and C8H17N2S4Hg+ were identified based on both the exact molecular mass and patterns of Hg stable isotope distributions detected by FTICR-MS. Density functional theory was used to predict the solution-phase structures of candidate molecules. These findings represent the first step to unambiguously identify specific DOM molecules in Hg binding, although future studies are warranted to further optimize and validate the methodology so as to explore detailed molecular compositions and structures of Hg–DOM complexes that affect biological uptake and transformation of Hg in the environment.

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Section: Introductionmentioning

confidence: 96%

Identification of Mercury and Dissolved Organic Matter Complexes Using Ultrahigh Resolution Mass Spectrometry

Chen

Johnston

Mann

et al. 2016

Environ. Sci. Technol. Lett.

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“…Protein interactions with small ligands (including co‐factors and drugs) Protein/peptide interactions with metals and ions Protein/peptide interactions with nucleic acids …”

Section: Introductionmentioning

confidence: 99%

Applications of isothermal titration calorimetry in pure and applied research—survey of the literature from 2010

Ghai

Falconer

Collins

2011

J of Molecular Recognition

165

108

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Isothermal titration calorimetry (ITC) is a biophysical technique for measuring the formation and dissociation of molecular complexes and has become an invaluable tool in many branches of science from cell biology to food chemistry. By measuring the heat absorbed or released during bond formation, ITC provides accurate, rapid, and label-free measurement of the thermodynamics of molecular interactions. In this review, we survey the recent literature reporting the use of ITC and have highlighted a number of interesting studies that provide a flavour of the diverse systems to which ITC can be applied. These include measurements of protein-protein and protein-membrane interactions required for macromolecular assembly, analysis of enzyme kinetics, experimental validation of molecular dynamics simulations, and even in manufacturing applications such as food science. Some highlights include studies of the biological complex formed by Staphylococcus aureus enterotoxin C3 and the murine T-cell receptor, the mechanism of membrane association of the Parkinson's disease-associated protein α-synuclein, and the role of non-specific tannin-protein interactions in the quality of different beverages. Recent developments in automation are overcoming limitations on throughput imposed by previous manual procedures and promise to greatly extend usefulness of ITC in the future. We also attempt to impart some practical advice for getting the most out of ITC data for those researchers less familiar with the method.

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“…The flow rate was 1 mL min -1 and the injection volume was 5 µL. working session, and a conditioning potential of -0.6 V was applied during 60 s before each measurement in order to prevent the adsorption of the thiol species on the gold surface [29], avoiding the loss of repeatability caused by the expected decrease of the electroactive surface area of the SPAuE. Chromatographic peak areas were quantified using GPES version 4.9 software in the case of Met, HCys, Cys and GSH whereas NAC area was quantified by chemometric deconvolution using a home-made Matlab program based on the fitting of overlapping signals to the exponentially modified gaussian (EMG) function [33].…”

Section: Chromatographic Systemmentioning

confidence: 99%

“…On the one hand, it has been reported that noble metals, like gold and silver, can be very convenient for the determination of aminothiols due to their affinity for thiol groups [27,[29][30][31]. On the other hand, the use of screen-printed electrodes (SPE) has been postulated as a very convenient alternative to conventional solid electrodes due to their intrinsic characteristics, such as low-cost character and three-electrode configuration printed on the same strip [32].…”

Section: Introductionmentioning

confidence: 99%

New discrimination tools for harvest year and varieties of white wines based on hydrophilic interaction liquid chromatography with amperometric detection

Pérez‐Ràfols

Subirats

Serrano

et al. 2019

Talanta

Self Cite

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A simple HPLC-EC method based on hydrophilic interaction liquid chromatography with amperometric detection through gold screen-printed electrodes has been developed and applied for the first time to the determination of aminothiols in white wines.Moreover, the coupling of the method with partial least squares discriminant analysis (PLS-DA) using the analysed aminothiols as biomarkers provides wine discrimination in terms of harvest year. White wine samples were directly injected and chromatographic areas, together with pH and redox potential values, allowed a successful discrimination of wines from different harvest years with a global classification rate of 97.8%. The developed HPLC-EC method also generated characteristic fingerprints that were combined with PLS-DA to classify wines according to three wine varieties, with a global classification rate of 95.3%.

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Binding of Hg2+ by Cys, Cys-Gly and reduced glutathione: Study by differential pulse voltammetry on rotating Au-disk electrode, electrospray ionization mass-spectrometry and isothermal titration calorimetry

Cited by 22 publications

References 30 publications

Identification of Mercury and Dissolved Organic Matter Complexes Using Ultrahigh Resolution Mass Spectrometry

Identification of Mercury and Dissolved Organic Matter Complexes Using Ultrahigh Resolution Mass Spectrometry

Applications of isothermal titration calorimetry in pure and applied research—survey of the literature from 2010

New discrimination tools for harvest year and varieties of white wines based on hydrophilic interaction liquid chromatography with amperometric detection

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