Binding of Folic Acid to Serum Proteins

Markkanen, T; Himanen, P; Rl, Pajula; Ruponen, S; Castrén, Olli

doi:10.1159/000208334

Cited by 14 publications

(4 citation statements)

References 8 publications

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“…The decrease was most prominent initially, indicating that other factors than an imbalance between intake and fetal requirement contributed to the phenomenon observed. Changes in folate metabolism during pregnancy have been attributed to rapid clearance to maternal tissue (lo), changes in the folate-protein binding in plasma (20), hormonal changes influencing metabolic pathways (24), and increased plasma clearance of folate by the kidneys (17). Plasma volume expansion may also contribute to changes in plasma folate concentrations (8).…”

Section: Discussionmentioning

confidence: 99%

Plasma and Red Blood Cell Folate During Normal Pregnancies

Magnus

1981

Acta Obstet Gynecol Scand

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Plasma and red cell folate concentrations (Lactobacillus casei activity) have been studied in 43 women during pregnancy. Iron, but no folic acid supplement was given. The plasma folate concentrations decreased significantly during pregnancy and were lower than in the reference cases at term. The red cell folate concentrations were compatible with the reference material during early pregnancy, and significantly higher than the reference material at term. A significant decrease was observed during the last two months of pregnancy. The plasma folate and the red cell folate concentrations at different stages of pregnancy were correlated and a relationship was demonstrated between these parameters at different stages of pregnancy. The hemoglobin concentrations were compatible with the reference material during early pregnancy and at term. One hundred and sixty-six women were also studied at term to evaluate the effect of repeated pregnancies on the folate status. Repeated pregnancies did not influence the plasma and red cell folate concentrations at term. We conclude that the folate intake is adequate to meet the increased requirements during pregnancy, and folic acid supplementation is therefore not recommended as a routine in normal pregnancies in this population.

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Section: Discussionmentioning

confidence: 99%

Plasma and Red Blood Cell Folate During Normal Pregnancies

Magnus

1981

Acta Obstet Gynecol Scand

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“…Increased folate binding to transferrin was also observed in myeloma, hyperthyreosis, and hepatitis. 72 Similar changes might occur in breast cancer and would explain the increased presence of serotransferrin, haptoglobin, and α2macroglobulin in the coronas formed around HFA-L-NPs in BrCr Pt serum in comparison to HV serum (Supplementary Table 4). The presence of serotransferrin on the particle surface might contribute to the enhanced uptake observed in MCF-7 cells.…”

Section: ■ Results and Discussionmentioning

confidence: 79%

“…Folate binding to plasma proteins is well documented . In healthy humans, folate binds to α2-macroglobulin, transferrin (and similar proteins), and to a lesser extent albumin and hemoglobins. − However, this seems to change in various conditions, for instance in the course of a normal pregnancy folate binding to transferrin increases and to α2-macroglobulin decreases. Increased folate binding to transferrin was also observed in myeloma, hyperthyreosis, and hepatitis .…”

Section: Resultsmentioning

confidence: 99%

“…In healthy humans, folate binds to α2-macroglobulin, transferrin (and similar proteins), and to a lesser extent albumin and hemoglobins. − However, this seems to change in various conditions, for instance in the course of a normal pregnancy folate binding to transferrin increases and to α2-macroglobulin decreases. Increased folate binding to transferrin was also observed in myeloma, hyperthyreosis, and hepatitis . Similar changes might occur in breast cancer and would explain the increased presence of serotransferrin, haptoglobin, and α2-macroglobulin in the coronas formed around HFA-L-NPs in BrCr Pt serum in comparison to HV serum (Supplementary Table 4).…”

Section: Resultsmentioning

confidence: 99%

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Different Serum, Different Protein Corona! The Impact of the Serum Source on Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles

et al. 2022

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The nanoparticle (NP) protein corona represents an interface between biological components and NPs, dictating their cellular interaction and biological fate. To assess the success of cellular targeting, NPs modified with targeting ligands are incubated with target cells in serum-free culture medium or in the presence of fetal bovine serum (FBS). In the former, the role of the corona is overlooked, and in the latter, the effects of a corona that does not represent the one forming in humans nor the respective disease state are considered. Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP. Successful in vitro targeting of breast cancer cells was only observed when NPs were incubated with target cells in the presence of BrCr Pt sera only. In such cases, the success of targeting was not attributed to the targeting ligand itself, but to the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera. This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP−cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.

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The Interaction of Folate Ligands with Macromolecules

Costa

Rothenberg

1977

The Year in Hematology

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Binding of Folic Acid to Serum Proteins

Cited by 14 publications

References 8 publications

Plasma and Red Blood Cell Folate During Normal Pregnancies

Plasma and Red Blood Cell Folate During Normal Pregnancies

Different Serum, Different Protein Corona! The Impact of the Serum Source on Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles

The Interaction of Folate Ligands with Macromolecules

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