Binding of Ethidium to Yeast tRNAPhe: A New Perspective on an Old Bromide

Holub, Oliver; Croney, John C.; Ishi, T.; Seifried, Steven E.; Jameson, David M.

doi:10.1007/978-3-642-56067-5_6

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Cited by 2 publications

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Frequency Domain Fluorometry: Theory and Application

Vetromile

Jameson

2013

Methods in Molecular Biology

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Frequency domain fluorometry is a widely utilized tool in the physical, chemical, and biological sciences. This chapter focuses on the theory of the method and the practical aspects required to carry out intensity decay, i.e., lifetime measurements on a modern frequency domain fluorometer. Several chemical/biological systems are utilized to illustrate data acquisition protocols. Data analysis procedures and methodologies are also discussed.

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Frequency Domain Fluorometry: Theory and Application

Vetromile

Jameson

2013

Methods in Molecular Biology

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Time-resolved methods in biophysics. 8. Frequency domain fluorometry: applications to intrinsic protein fluorescence

Ross

Jameson

2008

Photochem Photobiol Sci

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Time-resolved fluorescence spectroscopy is an indispensable tool in the chemical, physical and biological sciences for the study of fast kinetic processes in the subpicosecond to microsecond time scale. This review focuses on the development and modern implementation of the frequency domain approach to time-resolved fluorescence. Both intensity decay (lifetime) and anisotropy decay (dynamic polarization) will be considered and their application to intrinsic protein fluorescence will be highlighted. In particular we shall discuss the photophysics of the aromatic amino acids, tryptophan, tyrosine and phenylalanine, which are responsible for intrinsic protein fluorescence. This discussion will be illustrated with examples of frequency domain studies on several protein systems.

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Binding of Ethidium to Yeast tRNAPhe: A New Perspective on an Old Bromide

Cited by 2 publications

References 21 publications

Frequency Domain Fluorometry: Theory and Application

Frequency Domain Fluorometry: Theory and Application

Time-resolved methods in biophysics. 8. Frequency domain fluorometry: applications to intrinsic protein fluorescence

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