Binding of DNA Nucleobases and Nucleosides with Graphene

Varghese, Neenu; Mogera, Umesha; Govindaraj, A.; Das, Anindya; Maiti, Prabal K.; Sood, A. K.; Rao, C. N. R.

doi:10.1002/cphc.200800459

Cited by 498 publications

(433 citation statements)

References 20 publications

(34 reference statements)

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“…The interaction strengths of the different bases with graphene vary as it depends on the polarizability of the DNA bases [93,95]. Both theoretical and experimental studies report that guanine binds most strongly to graphene while A, T and C have lower and similar interaction strengths [93,[96][97][98][99][100].…”

Section: Detection Methods Based On Dna Adsorptionmentioning

confidence: 99%

Graphene nanodevices for DNA sequencing

2016

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Fast, cheap, and reliable DNA sequencing could be one of the most disruptive innovations of this decade as it will pave the way for personalised medicine. In pursuit of such technology, a variety of nanotechnology-based approaches have been explored and established including sequencing with nanopores. Due to its unique structure and properties, graphene provides interesting opportunities for the development of a new sequencing technology. In recent years, a wide range of creative ideas for graphene sequencers have been theoretically proposed and the first experimental demonstrations have begun to appear. Here, we review the different approaches to using graphene nanodevices for DNA sequencing, which involve DNA passing through graphene nanopores, nanogaps, and nanoribbons, and the physisorption of DNA on graphene nanostructures. We discuss the advantages and problems of each of these key techniques, and provide a perspective on the use of graphene in future DNA sequencing technology.

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Section: Detection Methods Based On Dna Adsorptionmentioning

confidence: 99%

Graphene nanodevices for DNA sequencing

2016

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“…Similar observations were made with 13 nm AuNPs capped with A15 ( Figure 2B) and for 5 nm AuNPs capped with A15 ( Figure 2C). While poly-T DNA binds to GO relatively weakly and poly-A binds strongly, [26][27][28] AuNP associated wrinkling was observed in both cases. For comparison, the original citrate capped AuNPs are not adsorbed by GO (Figure S1), and cannot be directly used for this study.…”

mentioning

confidence: 89%

Evaporation induced wrinkling of graphene oxide at the nanoparticle interface

Wang

Liu

2015

Nanoscale

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With the thickness of only a single atomic layer, graphene displays many interesting surface properties. A general observation is that wrinkles are formed on graphene oxide (GO) when it is dried in the presence of adsorbed inorganic nanoparticles. In this case, evaporation induced wrinkling is not an elastic deformation but is permanent. Understanding the nanoscale force of wrinkle formation is important for device fabrication and sensing. Herein, we employ surface functionalized gold nanoparticles (AuNPs) as a model system. All tested AuNPs induced wrinkling, including those capped by DNA, polymers and proteins. The size of AuNPs is less important compared to the property of solvent. Wrinkle formation is attributed to drying related capillary force acting on the GO surface, and a quantitative equation is derived. After drying, the adsorption affinity between GO and AuNPs is increased due to increased contact area.

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“…21 Fundamental studies on the interaction between DNA and GO were also carried out. 11,[22][23][24][25][26][27][28][29][30][31][32] GO is a loosely defined material, and the oxygen content can vary quite a lot depending on the preparation condition. The adsorption affinity of DNA is likely to depend on the oxygen content.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing

et al. 2016

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Fluorescently labeled DNA adsorbed on graphene oxide (GO) is a well-established sensing platform for detecting a diverse range of analytes. GO is a loosely defined material and its oxygen content may vary depending on the condition of preparation. Sometimes, a further reduction step is intentionally performed to decrease the oxygen content and the resulting material is called reduced GO (rGO). In this work, DNA adsorption and desorption from GO and rGO is systematically compared. Under the same salt concentration, DNA adsorbs slightly faster with a 2.6-fold higher capacity on rGO. At the same time, adsorbed DNA on rGO is more resistant to desorption induced by temperature, pH, urea, and organic solvents. Various lengths and sequences of DNA probes have been tested. When its complementary DNA (cDNA) is added as a model target analyte, the rGO sample has a higher signal-to-background and signalto-noise ratio, while the GO sample has a slightly higher absolute signal increase and faster signaling kinetics. Adsorbed DNAs on GO or rGO are still susceptible to non-specific displacement by other DNA and proteins. Overall, while rGO adsorb DNA more tightly, it allows efficient DNA sensing with an extremely low background signal.

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Binding of DNA Nucleobases and Nucleosides with Graphene

Cited by 498 publications

References 20 publications

Graphene nanodevices for DNA sequencing

Graphene nanodevices for DNA sequencing

Evaporation induced wrinkling of graphene oxide at the nanoparticle interface

Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing

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