Binding of Cellular Proteins to the Leader RNA of Equine Arteritis Virus

Archambault, Denis; St-Louis, Marie-Claude; Martin, Stéphanie

doi:10.1007/s11262-004-4589-6

Cited by 4 publications

(5 citation statements)

References 19 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cellular proteins appeared to bind to similar regions in the antileader of other arteriviruses including EAV, an observation that we recently confirmed [25]. In addition, we also observed cellular proteins to bind to the EAV leader RNA positive strand and showed that nt 140-206 are not necessary for this binding.…”

Section: Discussionsupporting

confidence: 83%

The Intraleader AUG Nucleotide Sequence Context is Important for Equine Arteritis Virus Replication

et al. 2006

Self Cite

View full text Add to dashboard Cite

The 5(-terminal leader sequence of the equine arteritis virus (EAV) genome contains an open reading frame (ORF) with an AUG codon in a suboptimal context for initiation of protein synthesis. To investigate the significance of this intraleader ORF (ILO), an expression plasmid was generated carrying a DNA copy of the subgenomic mRNA7 behind a T7 promoter. Capped RNA transcribed from this construct was shown to direct, in an in vitro translation system, the synthesis of leader peptide as well as N protein. Site-directed mutations aimed to either optimize or weaken the sequence context of the ILO start codon affected leader peptide synthesis as predicted; no peptide was detected when the initiation codon was incapacitated. Translation of the downstream N gene was inversely affected by leader peptide production, consistent with a ribosomal leaky scanning mechanism. To investigate the role of the leader peptide in the EAV replication life cycle we generated, using an infectious EAV cDNA clone, two mutant viruses in one of which the ILO start codon was in an optimal Kozak context for translation initiation while in the other the codon was again incapacitated. Surprisingly, both mutant viruses were equally viable and exhibited similar phenotypes in BHK-21 cells. However, their replication kinetics and viral yields were reduced relative to that of the wild-type parental virus, as were their plaque sizes. Importantly, the mutations introduced into the viruses appeared to be rapidly and precisely repaired upon passaging. Already after one viral passage a significant fraction of the viruses had regained the wild-type sequence as well as its phenotype. The results demonstrate that EAV replication is not dependent on the synthesis of the intraleader peptide. Rather, the leader peptide does not seem to have any function in the EAV life cycle. As we discuss, the available data indicate that the ILO 5( nucleotide sequence per se, not its functioning in translation initiation, is of critical importance for EAV replication.

show abstract

Section: Discussionsupporting

confidence: 83%

The Intraleader AUG Nucleotide Sequence Context is Important for Equine Arteritis Virus Replication

et al. 2006

Self Cite

View full text Add to dashboard Cite

show abstract

“…For arteriviruses, the common leader sequence of EAV sub-genomic viral RNAs, too, possesses the ability to interact several cellular proteins from the cytoplasmic fractions of Vero cells, likely for the modulation of EAV RNA synthesis [ 172 ].…”

Section: Cellular Factors Involved In the Virus Life Cyclementioning

confidence: 99%

Recent Progress in Studies of Arterivirus- and Coronavirus-Host Interactions

Zhong

Tan

Liu

2012

Viruses

View full text Add to dashboard Cite

Animal coronaviruses, such as infectious bronchitis virus (IBV), and arteriviruses, such as porcine reproductive and respiratory syndrome virus (PRRSV), are able to manifest highly contagious infections in their specific native hosts, thereby arising in critical economic damage to animal industries. This review discusses recent progress in studies of virus-host interactions during animal and human coronavirus and arterivirus infections, with emphasis on IBV-host cell interactions. These interactions may be directly involved in viral replication or lead to the alteration of certain signaling pathways, such as cell stress response and innate immunity, to facilitate viral replication and pathogenesis.

show abstract

“…6 Although thousands of articles have been published regarding the role of apoptosis in physiologic and pathologic processes in various species, there is a paucity of information regarding apoptosis in horses. To date, publications concerning apoptosis in horses have been limited to aspects of reproductive physiology, [7][8][9] exercise physiology, 10 chondrocyte growth, 11 virus infection, [12][13][14][15][16] melanocytic tumors, 17 and gastrointestinal tract disease. 18,19 To the authors' knowledge, there are no reports regarding apoptosis in the laminae of healthy horses or horses with laminitis.…”

Section: Conclusion and Clinicalmentioning

confidence: 99%