Binding of Bacteria to Lymphocyte Subpopulations

Teodorescu, Marius; Mayer, Eugene P.

doi:10.1016/s0065-2776(08)60838-x

Cited by 18 publications

(4 citation statements)

References 103 publications

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“…Both human and mice B cells have been shown to bind such different species of intracellular bacteria as Brucella spp. [23,24], Mycobacterium tuberculosis [25], or Chlamydia trachomatis [26] and to activate these to produce polyclonal immunoglobulins and/or cytokines [27,28]. Recently, it has been clarified that B cells can internalize intracellular bacteria through macropinocytosis [29,30].…”

Section: Introductionmentioning

confidence: 99%

B cell subsets are activated and produce cytokines during early phases of Francisella tularensis LVS infection

Plzakova

Kubelková²,

Kročová

et al. 2014

Microbial Pathogenesis

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Section: Introductionmentioning

confidence: 99%

B cell subsets are activated and produce cytokines during early phases of Francisella tularensis LVS infection

Plzakova

Kubelková²,

Kročová

et al. 2014

Microbial Pathogenesis

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“…(i) A total of 8 ml of blood was cultured in oxgall as described above. (ii) Then, 10 ml of blood was placed in an Isolator tube (Dupont, NEN Research Products, Boston, Mass. ), mixed thoroughly, and centrifuged in a fixed-angle rotor at 3,000 x g at 22°C for 30 min without the brake.…”

mentioning

confidence: 99%

Rapid diagnosis of typhoid fever through identification of Salmonella typhi within 18 hours of specimen acquisition by culture of the mononuclear cell-platelet fraction of blood

et al. 1990

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Detection of Salmonella typhi in blood by culture of the mononuclear cell-platelet layer was compared with other methods currently used for the diagnosis of typhoid fever. Colonies of S. typhi were present in all mononuclear cell-platelet layer-positive cultures within 18 h of plating and were identified within an additional 10 min by a coagglutination technique. In contrast, identification of all positive cultures by conventional blood culture required 3 days.

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“…Recently, we documented that Francisella infect in vitro murine and human B cell lines and in vivo murine peritoneal as well as spleen B cells [ 31 , 32 ]. Both human and mice B cells have been shown to bind various species of bacteria, including such intracellular bacteria as Brucella [ 33 , 34 ], Mycobacterium tuberculosis [ 35 ], and Chlamydia trachomatis [ 36 ]. Internalization into B cells of one of the Brucella subtypes, Brucella abortus , recently was shown to be promoted by functional microfilaments.…”

Section: Introductionmentioning

confidence: 99%

Entry of Francisella tularensis into Murine B Cells: The Role of B Cell Receptors and Complement Receptors

et al. 2015

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Francisella tularensis, the etiological agent of tularemia, is an intracellular pathogen that dominantly infects and proliferates inside phagocytic cells but can be seen also in non-phagocytic cells, including B cells. Although protective immunity is known to be almost exclusively associated with the type 1 pathway of cellular immunity, a significant role of B cells in immune responses already has been demonstrated. Whether their role is associated with antibody-dependent or antibody-independent B cell functions is not yet fully understood. The character of early events during B cell–pathogen interaction may determine the type of B cell response regulating the induction of adaptive immunity. We used fluorescence microscopy and flow cytometry to identify the basic requirements for the entry of F. tularensis into B cells within in vivo and in vitro infection models. Here, we present data showing that Francisella tularensis subsp. holarctica strain LVS significantly infects individual subsets of murine peritoneal B cells early after infection. Depending on a given B cell subset, uptake of Francisella into B cells is mediated by B cell receptors (BCRs) with or without complement receptor CR1/2. However, F. tularensis strain FSC200 ΔiglC and ΔftdsbA deletion mutants are defective in the ability to enter B cells. Once internalized into B cells, F. tularensis LVS intracellular trafficking occurs along the endosomal pathway, albeit without significant multiplication. The results strongly suggest that BCRs alone within the B-1a subset can ensure the internalization process while the BCRs on B-1b and B-2 cells need co-signaling from the co receptor containing CR1/2 to initiate F. tularensis engulfment. In this case, fluidity of the surface cell membrane is a prerequisite for the bacteria’s internalization. The results substantially underline the functional heterogeneity of B cell subsets in relation to F. tularensis.

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Binding of Bacteria to Lymphocyte Subpopulations

Cited by 18 publications

References 103 publications

B cell subsets are activated and produce cytokines during early phases of Francisella tularensis LVS infection

B cell subsets are activated and produce cytokines during early phases of Francisella tularensis LVS infection

Rapid diagnosis of typhoid fever through identification of Salmonella typhi within 18 hours of specimen acquisition by culture of the mononuclear cell-platelet fraction of blood

Entry of Francisella tularensis into Murine B Cells: The Role of B Cell Receptors and Complement Receptors

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