Binding of antibodies to nonhistone nucleoproteins on keratinocytes in suspensions

Velthuis, Peter; Wildschut, E. G.; Faille, H. Baart de la; Vloten, W. A. van; Weelden, Huib van; Kater, L.

doi:10.1007/bf00372615

Cited by 5 publications

(1 citation statement)

References 13 publications

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“…Both Ro and La antigens are present in normal human skin (13,14), as well as in various other tissues (1,9,15,16). Little is known about the factors that modulate the expression of Ro and La antigens in human skin; in previous studies, ultraviolet (UV) light has caused a translocation of both antigens from the cytoplasms and nucleus to the plasma membrane of keratinocytes (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27).…”

Section: Resultsmentioning

confidence: 99%

Heterogeneity in the expression of ro and la antigens in human skin

Niimi

Ioannides

Buyon

et al. 1995

Arthritis & Rheumatism

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Objective. To determine whether there are variations in the expression of Ro and La antigens in human skin.Methods. Levels of expression of Ro andor La antigens in 26 specimens of normal human skin (11 sun-exposed, 15 sun-protected) were measured by indirect immunofluorescence with monospecific antisera.Results. Levels of expression of both antigens varied by more than 2,000-fold in the skin of different individuals. There usually was a correlation between the levels of expression of Ro and La antigens in the same skin specimen. There was no correlation found between the levels of Ro or La antigen expression and sun exposure, nor was there a correlation found between levels of antigen expression and location of the skin on the body.Conclusion. There is a marked heterogeneity in the expression of both Ro and La antigens in the skin of different individuals. The present study findings suggest that the levels of expression of these antigens may play a role in the propensity of some individuals to develop anti-Ro or anti-La antibodies andor skin lesions associated with the presence of these antibodies.Antibodies to Ro and La RNP particles are frequently detected in the serum of patients with systemic lupus erythematosus (SLE) and other connective tissue diseases (1-3). The incidence of these antibodies is particularly high in forms of lupus erySupported in part by USPHS research grants P-30-CA-16087, AR-39749, and R01-AM-27663-09, and by funds from the Rudolph Baer Foundation.Yayoi Niimi, MD: Nippon Medical School, Tokyo, Japan; Demetris Ioannides, MD: Aristotelian University Medical School, Thessaloniki, Greece; Jill Buyon, MD: Hospital for Joint Diseases, New York University School of Medicine, New York, NY; JeanClaude Bystryn, MD: New York University School of Medicine, New York, NY.Address reprint requests to Jean-Claude Bystryn, MD, NYU Medical Center, 560 First Avenue, New York, NY 10016.Submitted for publication April 19, 1994; accepted in revised form March 7, 1995. thematosus that are associated with photosensitivity, such as subacute cutaneous LE (SCLE) and neonatal LE, in which Ro and La antibodies are present at very high titers in >80% and >60% of the patients, respectively (4-7). The reason for this is not yet known. One possibility is that sun exposure increases the expression of Ro and/or La antigens in human skin. To address this issue, we studied the levels of expression of these antigens in human skin, obtained from both sun-exposed and sun-protected areas of the body. Unexpectedly, the results showed that, although there was little correlation between sun exposure and the expression of Ro and La antigens in human skin, there was a striking variation in the expression of these antigens between different individuals. MATERIALS AND METHODSAntisera. Two antisera were used in this study; one contained antibodies that react predominantly against Ro polypeptides, and the other contained antibodies with predominant reactivity against La polypeptides. These sera were selected from among several hundred...

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Section: Resultsmentioning

confidence: 99%

Heterogeneity in the expression of ro and la antigens in human skin

Niimi

Ioannides

Buyon

et al. 1995

Arthritis & Rheumatism

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Stimulation of anti-RNP antibody binding to cultured keratinocytes by estradiol

1991

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Antibodies to extractable nuclear antigens (ENA) are frequently demonstrated in the serum of patients with cutaneous lupus erythematosus. To gain a better understanding of the influence of sex hormones on cutaneous lupus, we investigated the in vitro binding of anti-ENA antibodies to cultured human keratinocytes. Estradiol-beta augmented the binding of anti-RNP, anti-SS-A/Ro, and anti-SS-B/La antibodies to cultured cells, but produced no enhancement of the binding of anti-Sm antibodies. In addition, we examined the effects of 16-alpha-hydroxyestrone, a highly estrogenic metabolite, on the binding of anti-ENA antibodies to cultured cells. This agent did not produce any augmentation of binding. Based on these experiments, we discuss the influence of estradiol on cutaneous lupus.

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