Binding motifs predict major histocompatibility complex class II-restricted epitopes in the Sendai virus M protein

Cole, Gerald A.; Tao, Tien-Wen; Hogg, Twala L.; Ryan, Kevin; Woodland, David L.

doi:10.1128/jvi.69.12.8057-8060.1995

Cited by 11 publications

(5 citation statements)

References 34 publications

(42 reference statements)

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“…suggested as responsible for the induction of memory T cells that produce Th2-like rather than Th1-like cytokines (6,32,40). Class II-restricted CTL responses have been noted for a number of other RNA viruses, including parainfluenza and Sendai viruses (4,10,14), measles viruses (38,39), and coronavirus (19) in the family Paramyxoviridae and recently for influenza virus (13,34) in the family Orthomyxoviridae. Limiting dilution analysis of parainfluenza virus-specific CD4 ϩ and CD8 ϩ T lymphocytes from the peripheral blood of adults showed relatively high frequencies for both CD4 ϩ (1:4,700) and CD8 ϩ (1:2,500) CTLps (10).…”

Section: Discussionmentioning

confidence: 99%

“…Measles virus infection in humans has been shown to induce both HLA class I-and class II-restricted CTLs specific to the transmembrane fusion glycoprotein (39). In mice, Sendai virus-specific class II-restricted T-cell responses have been investigated and defined (4,13). Class II-restricted, Sendai virus-specific T-cell hybridomas have been generated, and their reactivity has been characterized to the level of specific peptide sequences in the matrix protein (4).…”

Section: Discussionmentioning

confidence: 99%

“…In mice, Sendai virus-specific class II-restricted T-cell responses have been investigated and defined (4,13). Class II-restricted, Sendai virus-specific T-cell hybridomas have been generated, and their reactivity has been characterized to the level of specific peptide sequences in the matrix protein (4). Similarly, class II-restricted (CD4 ϩ ) precursors were detected in mice during the acute immune response to Sendai virus (13,14).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Cytotoxic T-Lymphocyte Precursor Frequencies in BALB/c Mice after Acute Respiratory Syncytial Virus (RSV) Infection or Immunization with a Formalin-Inactivated RSV Vaccine

Tripp

Anderson

1998

J. Virol.

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A better understanding of the immune response to live and formalin-inactivated respiratory syncytial virus (RSV) is important for developing nonlive vaccines. In this study, major histocompatibility complex (MHC) class I- and II-restricted, RSV-specific cytotoxic T-lymphocyte precursor (CTLp) frequencies were determined in bronchoalveolar lavage (BAL) samples and spleen lymphocytes of BALB/c mice intranasally infected with live RSV or intramuscularly inoculated with formalin-inactivated RSV (FI-RSV). After RSV infection, both class I- and class II-restricted CTLps were detected by day 4 or 5 postinfection (p.i.). Peak CTLp frequencies were detected by day 7 p.i. The class II-restricted CTLp frequencies in the BAL following RSV infection were less than class I-restricted CTLp frequencies through day 14 p.i., during which class I-restricted CTLp frequencies remained elevated, but then declined by 48 days p.i. The frequencies of class II-restricted CTLps in the BAL were 2- to 10-fold less than those of class I-restricted CTLps. For spleen cells, frequencies of both MHC class I- and II-restricted CTLps to live RSV were similar. In contrast, class II-restricted CTLps predominated in FI-RSV-vaccinated mice. RSV challenge of vaccinated mice resulted in an increase in the frequency of class I-restricted CTLps at day 3 p.i. but did not enhance class II-restricted CTLp frequencies. These studies demonstrate differences in the CTLp response to live RSV infection compared with FI-RSV immunization and help define possible mechanisms of enhanced disease after FI-RSV immunization. In addition, these studies provide a quantitative means to address potential vaccine candidates by examining both MHC class I- and II-restricted CTLp frequencies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Cytotoxic T-Lymphocyte Precursor Frequencies in BALB/c Mice after Acute Respiratory Syncytial Virus (RSV) Infection or Immunization with a Formalin-Inactivated RSV Vaccine

Tripp

Anderson

1998

J. Virol.

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“…Different algorithms for the prediction of binding to major histocompatibility complex (MHC) class I molecules, in addition to search for the less restricted MHC class II motifs, represent valuable and time-saving experimental approaches to locate potential T-cell epitopes within an antigen [19]. We tested various MHC class I prediction engines for the prediction of T-cell epitopes within MPB70.…”

Section: Introductionmentioning

confidence: 99%

T‐ and B‐Cell Epitopes in the Secreted Mycobacterium bovis Antigen MPB70 in Mice

et al. 2003

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MPB70 is a soluble secreted protein highly expressed in Mycobacterium bovis and strains of bacille Calmette±Gue Ârin (BCG); as such, it is a candidate for subunit and DNA vaccines against tuberculosis. MPB70 was screened for T-cell epitopes in four different inbred mouse strains. Major histocompatibility complex (MHC) H-2 b -expressing mice (C57BL/6) secreted interferon-g (IFN-g) after stimulation with peptides from the regions 1±20, 41±50, 81±110, 121±150 and 161±193 of the MPB70 sequence. H-2 db mouse (B6D2) splenocytes secreted IFN-g after stimulation with some of the same peptides, whereas H-2 d mice (BALB/c and DBA/2) did not secrete IFN-g upon stimulation with the peptides. Sera from H-2 db mice immunized with native MPB70 in incomplete Freund's adjuvant (IFA), mpb70 DNA or live BCG Moreau were found to contain antibodies against the native MPB70 antigen. H-2 db mice immunized with native MPB70 in IFA exhibited high titres of peptide-reactive immunoglobulin G1 (IgG1) antibodies, whereas DNA-immunized mice reacted with IgG2a antibodies against some of the same peptides. As some of the epitopes recognized by mouse T and B cells have previously been found to stimulate immune responses in humans, cattle and rabbits, we conclude that these epitopes may be good general epitopes for the stimulation of T-and B-cell responses and candidates for a DNA vaccine with a broad applicability.

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“…Peptides. All of the peptides used in this study, with the exception of Sendal virus HN41s-430 and SEB121q36, have been previously described (16)(17)(18)(19)24). Both HN41s.430 (GDR_VYIYTR.SSGWHSP) and SEB121_136 (HNGNQLDKYP,.SITVILV) will be described in separate studies (Cole, G.A., T.L.…”

mentioning

confidence: 99%

Major histocompatibility complex class II-associated peptides control the presentation of bacterial superantigens to T cells.

Wen

Cole

Surman

et al. 1996

The Journal of Experimental Medicine

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SummaryRecent studies have shown that only a subset of major histocompatibility complex (MHC) class II molecules are able to present bacterial superantigens to T cells, leading to the suggestion that class II-associated peptides may influence superantigen presentation. Here, we have assessed the potential role of peptides on superantigen presentation by (a) analyzing the ability of superantigens to block peptide-specific T cell responses and (b) analyzing the ability of individual peptides to promote superantigen presentation on I-Ab-expressing T2 cells that have a quantitative defect in antigen processing. A series of peptides is described that specifically promote either toxic shock syndrome toxin (TSST) 1 or staphylococcal enterotoxin A (SEA) presentation. Whereas some peptides promoted the presentation of TSST-1 (almost 5,000-fold in the case of one peptide), other peptides promoted the presentation of SEA. These data demonstrate that MHC class II-associated peptides differentially influence the presentation of bacterial superantigens to T cells.

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Binding motifs predict major histocompatibility complex class II-restricted epitopes in the Sendai virus M protein

Cited by 11 publications

References 34 publications

Cytotoxic T-Lymphocyte Precursor Frequencies in BALB/c Mice after Acute Respiratory Syncytial Virus (RSV) Infection or Immunization with a Formalin-Inactivated RSV Vaccine

Cytotoxic T-Lymphocyte Precursor Frequencies in BALB/c Mice after Acute Respiratory Syncytial Virus (RSV) Infection or Immunization with a Formalin-Inactivated RSV Vaccine

T‐ and B‐Cell Epitopes in the Secreted Mycobacterium bovis Antigen MPB70 in Mice

Major histocompatibility complex class II-associated peptides control the presentation of bacterial superantigens to T cells.

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