Binding motifs ofCBP2 a potential cell surface target for carcinoma cells

Sauk, John J.; Coletta, Ricardo D.; Norris, Kathleen; Hebert, Carla

doi:10.1002/(sici)1097-4644(20000801)78:2<251::aid-jcb8>3.0.co;2-g

Cited by 17 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding this later potential, an important advantage is that HSP47 has procollagen as its exclusive target, though it is important to consider that HSP47 is expected to locate in the cytosol, cycling between ER compartments and Golgi complex, and an effective delivery system is needed. However, previous studies have demonstrated that cell lines derived from head and neck cancers may express HSP47 in the cell surface anchored to CD9 16,29 …”

Section: Discussionmentioning

confidence: 99%

Overexpression of heat‐shock protein 47 impacts survival of patients with oral squamous cell carcinoma

da Costa,

Dourado,

de Moraes

et al. 2023

J Oral Pathology Medicine

Self Cite

View full text Add to dashboard Cite

BackgroundThe expression of heat‐shock protein 47 (HSP47) has been linked to collagen synthesis control and implicated in fibrotic disorders, but more recent studies have demonstrated its role in solid tumors. In this study, we explored the prognostic impact of HSP47 in oral squamous cell carcinomas (OSCC) and determined the in vitro effects of its loss‐of‐function on viability, proliferation, migration, invasion, and resistance to cisplatin of OSCC cells.MethodsThe HSP47 expression in tumor samples was assessed by immunohistochemistry in two independent cohorts totaling 339 patients with OSCC, and protein levels were associated with clinicopathological features and survival outcomes. The OSCC cell lines HSC3 and SCC9 were transduced with lentivirus expressing short hairpin RNA to stably silence HSP47 and used in assays to measure cellular viability, proliferation, migration, and invasion.ResultsHSP47 was overexpressed in OSCC samples, and its overexpression was significantly and independently associated with poor disease‐specific survival and shortened disease‐free survival in both OSCC cohorts. The knockdown of HSP47 showed no effects on cell viability or cisplatin sensitivity, but impaired significantly proliferation, migration, and invasion of OSCC cells, with stronger effects on SCC9 cells.ConclusionOur results show a significant prognostic impact of HSP47 overexpression in OSCC and reveal that HSP47 inhibition impairs the proliferation, migration, and invasion of OSCC cells. HSP47 may represent a potential therapeutic target for OSCC.

show abstract

Section: Discussionmentioning

confidence: 99%

Overexpression of heat‐shock protein 47 impacts survival of patients with oral squamous cell carcinoma

da Costa,

Dourado,

de Moraes

et al. 2023

J Oral Pathology Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Additionally, each of these targets has ligands that can be used to actively target SPIO nanoparticles. Specifically, HER2 affibody, cyclic RGD, and the LDS affinity peptide ( Table 1 ) were selected as ligands for targeting HER2/neu, αvβ 3 integrin, and HSP47 respectively 29 30 31 32 33 34 . A set of four lanthanide-doped SPIO nanoparticles (Ho, Sm, Gd, and Er) were synthesized.…”

mentioning

confidence: 99%

Quantitative Comparison of Tumor Delivery for Multiple Targeted Nanoparticles Simultaneously by Multiplex ICP-MS

Elias

Crayton

Warden-Rothman

et al. 2014

Sci Rep

View full text Add to dashboard Cite

Given the rapidly expanding library of disease biomarkers and targeting agents, the number of unique targeted nanoparticles is growing exponentially. The high variability and expense of animal testing often makes it unfeasible to examine this large number of nanoparticles in vivo. This often leads to the investigation of a single formulation that performed best in vitro. However, nanoparticle performance in vivo depends on many variables, many of which cannot be adequately assessed with cell-based assays. To address this issue, we developed a lanthanide-doped nanoparticle method that allows quantitative comparison of multiple targeted nanoparticles simultaneously. Specifically, superparamagnetic iron oxide (SPIO) nanoparticles with different targeting ligands were created, each with a unique lanthanide dopant. Following the simultaneous injection of the various SPIO compositions into tumor-bearing mice, inductively coupled plasma mass spectroscopy was used to quantitatively and orthogonally assess the concentration of each SPIO composition in serial blood and resected tumor samples.

show abstract

“…Only a modest amount of time, effort, and resources are needed to screen a library displaying a billion different peptides GENERAL CONSIDERATIONS by affinity selection. Peptide ligands have been isolated to a wide array of proteins, such as 14-3-3 proteins (1, In this article, we present simple, step-by-step proto-2), calmodulin (3)(4)(5), cell surface receptors (6-10), EH cols for screening phage-displayed combinatorial pepdomains (11)(12)(13), heat-shock proteins (14,15), integrins tide libraries. Many types of protein targets, including (16), PDZ domains (17,18), SH3 domains (19)(20)(21), vasenzymes (34,35), have been used successfully to affinity cular endothelial growth factor (VEGF) (22, 23), viral select binding phage.…”

mentioning

confidence: 99%