Binding kinetics of grouper nervous necrosis viruses with functionalized antimicrobial peptides by nanomechanical detection

Kuan, Shu; Chi, Shau-Chi; Cheng, Yung-Jen; Chia, Ta-Jui; Huang, Long-Sun

doi:10.1016/j.bios.2011.10.003

Cited by 10 publications

(7 citation statements)

References 40 publications

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“…It is assumed that the rate constant for binding kinetics (association) is given by k a , whereas the rate constant for unbinding kinetics (dissociation) is given by k d . The rate of pair binding between the capture antibodies and antigens was given due to the random conjugation by [32]:

\frac{d N}{d t} = k_{a} Φ (N) C_{s} - k_{d} N

where N is the number of binding pairs on microcantilever surface as a function of time, C s is the CRP concentration on the sensing surface, Φ( N ) gives the remaining pairs available for binding. For a small ratio of k d /k a with high affinity antibody–antigen interaction, the second term in the right hand side indicating dissociation process can be neglected [33].…”

Section: Resultsmentioning

confidence: 99%

Electrical Detection of C-Reactive Protein Using a Single Free-Standing, Thermally Controlled Piezoresistive Microcantilever for Highly Reproducible and Accurate Measurements

Yen

Lai

Hong

et al. 2013

Sensors

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This study demonstrates a novel method for electrical detection of C-reactive protein (CRP) as a means of identifying an infection in the body, or as a cardiovascular disease risk assay. The method uses a single free-standing, thermally controlled piezoresistive microcantilever biosensor. In a commonly used sensing arrangement of conventional dual cantilevers in the Wheatstone bridge circuit, reference and gold-coated sensing cantilevers that inherently have heterogeneous surface materials and different multilayer structures may yield independent responses to the liquid environmental changes of chemical substances, flow field and temperature, leading to unwanted signal disturbance for biosensing targets. In this study, the single free-standing microcantilever for biosensing applications is employed to resolve the dual-beam problem of individual responses in chemical solutions and, in a thermally controlled system, to maintain its sensor performance due to the sensitive temperature effect. With this type of single temperature-controlled microcantilever sensor, the electrical detection of various CRP concentrations from 1 μg/mL to 200 μg/mL was performed, which covers the clinically relevant range. Induced surface stresses were measured at between 0.25 N/m and 3.4 N/m with high reproducibility. Moreover, the binding affinity (KD) of CRP and anti-CRP interaction was found to be 18.83 ± 2.99 μg/mL, which agreed with results in previous reported studies. This biosensing technique thus proves valuable in detecting inflammation, and in cardiovascular disease risk assays.

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\frac{d N}{d t} = k_{a} Φ (N) C_{s} - k_{d} N

Section: Resultsmentioning

confidence: 99%

Electrical Detection of C-Reactive Protein Using a Single Free-Standing, Thermally Controlled Piezoresistive Microcantilever for Highly Reproducible and Accurate Measurements

Yen

Lai

Hong

et al. 2013

Sensors

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“…Although they usually destroy the cell membrane of the target cell directly, the action mechanism remains the subject of much scientific debate. However, their function is mainly to help the body fight against harmful substances within the body [ 22 ], interfere with bacterial proliferation [ 23 ], be a anti-nociceptor [ 24 ], and promote wound healing [ 25 ]. Recently, MSPs were also found to be useful in the treatment of liver cancer [ 26 ], cervical cancer [ 27 ], and fibrosarcoma [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

MSP-4, an Antimicrobial Peptide, Induces Apoptosis via Activation of Extrinsic Fas/FasL- and Intrinsic Mitochondria-Mediated Pathways in One Osteosarcoma Cell Line

et al. 2018

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Osteosarcoma (OS) is a common malignant bone cancer. The relatively high density of a person’s bone structure means low permeability for drugs, and so finding drugs that can be more effective is important and should not be delayed. MSPs are marine antimicrobial peptides (AMP) and natural compounds extracted from Nile tilapia (Oreochromis niloticus). MSP-4 is a part of the AMPs series, with the advantage of having a molecular weight of about 2.7-kDa and anticancer effects, although the responsible anticancer mechanism is not very clear. The goal of this study is to determine the workings of the mechanism associated with apoptosis resulting from MSP-4 in osteosarcoma MG63 cells. The study showed that MSP-4 significantly induced apoptosis in MG63 cells, with Western blot indicating that MSP-4 induced this apoptosis through an intrinsic pathway and an extrinsic pathway. Thus, a pretreatment system with a particular inhibitor of Z-IETD-FMK (caspase-8 inhibitor) and Z-LEHD-FMK (caspase-9 inhibitor) significantly attenuated the cleavage of caspase-3 and prevented apoptosis. These observations indicate that low concentrations of MSP-4 can help induce the apoptosis of MG63 through a Fas/FasL- and mitochondria-mediated pathway and suggest a potentially innovative alternative to the treatment of human osteosarcoma.

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“…Meanwhile, the rate constant for binding kinetics (association) is given by k a , whereas the rate constant for unbinding kinetics (dissociation) is given by k d . The rate of binding between the capture antibodies and small molecules was obtained by random conjugation using the following equation [ 26 ].

where N is the number of binding molecules on the microcantilever surface as a function of time, C s is the valproic acid drug concentration on the sensing surface and Φ(N) gives the number of remaining pairs available for binding.…”

Section: Resultsmentioning

confidence: 99%

“…It was assumed that the response (σ) of the microcantilever from the conjugation complex can effectively contribute to the generation of surface stress. In practice, the correlation can be described in an equilibrium state by an input of dN/dt = 0 and in terms of the saturation level in the equilibrium state to derive another form of kinetic basis system [ 26 ]:

…”

Section: Resultsmentioning

confidence: 99%

Direct Determination of a Small-Molecule Drug, Valproic Acid, by an Electrically-Detected Microcantilever Biosensor for Personalized Diagnostics

et al. 2015

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Direct, small-molecule determination of the antiepileptic drug, valproic acid, was investigated by a label-free, nanomechanical biosensor. Valproic acid has long been used as an antiepileptic medication, which is administered through therapeutic drug monitoring and has a narrow therapeutic dosage range of 50–100 μg·mL−1 in blood or serum. Unlike labeled and clinically-used measurement techniques, the label-free, electrical detection microcantilever biosensor can be miniaturized and simplified for use in portable or hand-held point-of-care platforms or personal diagnostic tools. A micromachined microcantilever sensor was packaged into the micro-channel of a fluidic system. The measurement of the antiepileptic drug, valproic acid, in phosphate-buffered saline and serum used a single free-standing, piezoresistive microcantilever biosensor in a thermally-controlled system. The measured surface stresses showed a profile over a concentration range of 50–500 μg·mL−1, which covered the clinically therapeutic range of 50–100 μg·mL−1. The estimated limit of detection (LOD) was calculated to be 45 μg·mL−1, and the binding affinity between the drug and the antibody was measured at around 90 ± 21 μg·mL−1. Lastly, the results of the proposed device showed a similar profile in valproic acid drug detection with those of the clinically-used fluorescence polarization immunoassay.

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Binding kinetics of grouper nervous necrosis viruses with functionalized antimicrobial peptides by nanomechanical detection

Cited by 10 publications

References 40 publications

Electrical Detection of C-Reactive Protein Using a Single Free-Standing, Thermally Controlled Piezoresistive Microcantilever for Highly Reproducible and Accurate Measurements

Electrical Detection of C-Reactive Protein Using a Single Free-Standing, Thermally Controlled Piezoresistive Microcantilever for Highly Reproducible and Accurate Measurements

MSP-4, an Antimicrobial Peptide, Induces Apoptosis via Activation of Extrinsic Fas/FasL- and Intrinsic Mitochondria-Mediated Pathways in One Osteosarcoma Cell Line

Direct Determination of a Small-Molecule Drug, Valproic Acid, by an Electrically-Detected Microcantilever Biosensor for Personalized Diagnostics

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