Binding Interactions Between α-glucans from Lactobacillus reuteri and Milk Proteins Characterised by Surface Plasmon Resonance

Diemer, Silja Kej; Svensson, Birte; Babol, Linnea N.; Cockburn, Darrell; Grijpstra, Pieter; Dijkhuizen, Lubbert; Folkenberg, Ditte Marie; Garrigues, Christel; Ipsen, Richard

doi:10.1007/s11483-012-9260-5

Cited by 16 publications

(11 citation statements)

References 38 publications

(48 reference statements)

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“…Compared to β-linkages, α-linkages result in more flexible polymers 22 . It has been shown that molecular properties including type of linkages of the polysaccharides strongly impact the interactions with proteins 71 . Biological activities such as anti-tumor and apoptosis inducing activity by polysaccharides are strongly associated with their structures.…”

Section: Resultsmentioning

confidence: 99%

“…Biological activities such as anti-tumor and apoptosis inducing activity by polysaccharides are strongly associated with their structures. It has been reported that anti-tumor polysaccharides mainly show β-1,3-linkages and polysaccharides containing mainly β-1,6- linkages have less anti-tumor activities 71,72 . Another study mainly focused on the structure of β-glucan demonstrated that (1,3)- β-glucan with the (1,6)- β-glucan branches increased immuno competent cell activity and have an important role in anti-tumor activity of the polysaccharides 67 .…”

Section: Resultsmentioning

confidence: 99%

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The relationship between the structural characteristics of lactobacilli-EPS and its ability to induce apoptosis in colon cancer cells in vitro

Tukenmez

Aktaş

Aslım

et al. 2019

Sci Rep

118

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Colon cancer is one of the most common cancer around the world. Exopolysaccharides (EPSs) produced by lactobacilli as potential prebiotics have been found to have an anti-tumor effect. In this study, lyophilized EPSs of four Lactobacillus spp. for their impact on apoptosis in colon cancer cells (HT-29) was evaluated using flow cytometry. The relationship between capability of a lactobacilli-EPS to induce apoptosis and their monosaccharide composition, molecular weight (MW), and linkage type was investigated by HPLC, SEC, and NMR, respectively. Changes in apoptotic-markers were examined by qPCR and Western Blotting. EPSs were capable of inhibiting proliferation in a time-dependent manner and induced apoptosis via increasing the expression of Bax, Caspase 3 and 9 while decreasing Bcl-2 and Survivin. All EPSs contained mannose, glucose, and N-acetylglucosamine with different relative proportions. Some contained arabinose or fructose. MW ranged from 10 2 –10 4 Da with two or three fractions. EPS of L. delbrueckii ssp. bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction. In conclusion, lactobacilli-EPSs inhibit cell proliferation in HT-29 via apoptosis. Results suggest that a relationship exists between the ability of EPS to induce apoptosis and its mannose and glucose composition.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

The relationship between the structural characteristics of lactobacilli-EPS and its ability to induce apoptosis in colon cancer cells in vitro

Tukenmez

Aktaş

Aslım

et al. 2019

Sci Rep

118

View full text Add to dashboard Cite

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“…Typically these analyses are conducted with oligosaccharide analogues resembling the targeted polysaccharide. It is also possible, however, to gain insight into binding of soluble polysaccharides by using SPR (Diemer et al 2012).…”

Section: Methodologies Relevant For Detection Identification and Chamentioning

confidence: 99%

Analysis of surface binding sites (SBSs) in carbohydrate active enzymes with focus on glycoside hydrolase families 13 and 77 — a mini-review

et al. 2014

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Surface binding sites (SBSs) interact with carbohydrates outside of the enzyme active site. They are frequently situated on catalytic domains and are distinct from carbohydrate binding modules (CBMs). SBSs are found in a variety of enzymes and often seen in crystal structures. Notably about half of the > 45 enzymes (in 17 GH and two GT families) with an identified SBS are from GH13 and a few from GH77, both belonging to clan GH-H of carbohydrate active enzymes. The many enzymes of GH13 with SBSs provide an opportunity to analyse their distribution within this very large and diverse family. SBS containing enzymes in GH13 are spread among 15 subfamilies (two were not assigned a subfamily). Comparison of these SBSs reveals a complex evolutionary history with evidence of conservation of key residues and/or structural location between some SBSs, while others are found at entirely distinct structural locations, suggesting convergent evolution. An array of investigations of the two SBSs in barley α-amylase demonstrated they play different functional roles in binding and degradation of polysaccharides. MalQ from Escherichia coli is an α-1,4-glucanotransferase of GH77, a family that is known to have at least one member that contains an SBS. Whereas MalQ is a single domain enzyme lacking CBMs, its plant orthologue DPE2 contains two N-terminal CBM20s. Surface plasmon resonance binding studies showed that MalQ and DPE2 have a similar affinity for β-cyclodextrin and that MalQ binds malto-oligosaccharides of >DP4 at a second site in competition with β-cyclodextrin yielding a stoichiometry >1. This suggests that MalQ may have an SBS, though its structural location remains unknown.

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“…A N U S C R I P T 4 JFR1 forms a network with heat-aggregated whey proteins at low pH [22]. Surface plasmon resonance studies have shown that the binding capability of HoPSs with milk proteins varies according to the type of glycosidic linkage, degree of branching and molecular size, and that the binding decreased with increasing pH from 4.0 to 5.5 [23,24]. Bacterial EPSs have also been used as thickener and suspension stabilizer in many pharmaceutical creams and suspensions, and more recently as a potential drug delivery system [3,25,26].…”

Section: A C C E P T E D Mmentioning

confidence: 99%

Interaction between structurally different heteroexopolysaccharides and β-lactoglobulin studied by solution scattering and analytical ultracentrifugation

Khan¹,

Birch

Calsteren

et al. 2018

International Journal of Biological Macromolecules

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Despite a very large number of bacterial exopolysaccharides have been reported, detailed knowledge on their molecular structures and associative interactions with proteins is lacking. Small-angle X-ray scattering, dynamic light scattering and analytical ultracentrifugation (AUC) were used to characterize the interactions of six lactic acid bacterial heteroexopolysaccharides (HePS-1-HePS-6) with β-lactoglobulin (BLG). Compared to free HePSs, a large increase in the X-ray radius of gyration R, maximum length L and hydrodynamic diameter d of HePS-1-HePS-4 mixed with BLG revealed strong aggregation, the extent of which depended on the compact conformation and degree of branching of these HePSs. No significant effects were observed with HePS-5 and HePS-6. Turbidity and AUC analyses showed that both soluble and insoluble BLG-HePS complexes were formed. The findings provide new insights into the role of molecular structures in associative interactions between HePSs and BLG which has relevance for various industrial applications.

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Binding Interactions Between α-glucans from Lactobacillus reuteri and Milk Proteins Characterised by Surface Plasmon Resonance

Cited by 16 publications

References 38 publications

The relationship between the structural characteristics of lactobacilli-EPS and its ability to induce apoptosis in colon cancer cells in vitro

The relationship between the structural characteristics of lactobacilli-EPS and its ability to induce apoptosis in colon cancer cells in vitro

Analysis of surface binding sites (SBSs) in carbohydrate active enzymes with focus on glycoside hydrolase families 13 and 77 — a mini-review

Interaction between structurally different heteroexopolysaccharides and β-lactoglobulin studied by solution scattering and analytical ultracentrifugation

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