Binding induced strand displacement amplification for homogeneous protein assay

Li, Yubin; Liu, Sheng; Zhao, Zike; Zheng, Yuner; Wang, Zirui

doi:10.1016/j.talanta.2016.11.047

Cited by 16 publications

(5 citation statements)

References 60 publications

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“…It exhibited an excellent sensitivity with LOD in the femtomolar range. For comparison, other strand-displacement amplification methods with hairpin probes exhibited detection limits in the picomolar (pM) to attomolar (aM) ranges. − Those studies amplified the miRNA targets, of which the secondary structure of RNA was not a major obstacle. Both the amplification and detection technologies are the main contributing factors to the different detection limits of those studies.…”

Section: Resultsmentioning

confidence: 99%

Hybridization Cascade Plus Strand-Displacement Isothermal Amplification of RNA Target with Secondary Structure Motifs and Its Application for Detecting Dengue and Zika Viruses

et al. 2019

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Biological RNA generally comprises secondary structure motifs which cause a problem for target RNA detection by isothermal amplification methods. The complexity of the secondary structures makes RNA targets inaccessible for probe hybridization, resulting in decreased sensitivity and selectivity. This is particularly important because the hybridization step of the isothermal amplification method requires a limited temperature range. A strand-displacement strategy can enhance the hybridization efficiency between the probe and target RNA with secondary structure motifs. A short, singlestranded segment within the secondary structure can be used as a toehold for initiating strand displacement. The strategy has been used to establish a highly sensitive isothermal amplification by a combination of a hairpin probe hybridization and stranddisplacement amplification. The hairpin probe is placed on the single-stranded segment of the target RNA's secondary structure to initiate strand displacement. The probe's hybridization cascade provides a template for exponential amplification in two directions by strand-displacement amplification, designated hybridization cascade plus strand-displacement isothermal amplification (HyCaSD). The method requires no reverse transcription step. HyCaSD showed an excellent sensitivity with the limit of detection in the femtomolar (fM) range for synthetic targets as well as viral RNAs. Discrimination between DENV/ ZIKV and JEV/CHIKV was successfully demonstrated using real viruses. Therefore, HyCaSD is a promising platform that can be further developed for diagnostic applications.

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Section: Resultsmentioning

confidence: 99%

Hybridization Cascade Plus Strand-Displacement Isothermal Amplification of RNA Target with Secondary Structure Motifs and Its Application for Detecting Dengue and Zika Viruses

et al. 2019

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“…This process highly enhances detection sensitivity . While enzyme-based signal amplification techniques such as loop-mediated isothermal amplification (LAMP), strand displacement amplification (SDA), and RCA , can detect minimal target quantities in vitro, their utility in living cells is constrained by their dependence on external enzymes.…”

Section: Endogenous Enzyme-assisted Signal Amplificationmentioning

confidence: 99%

Amplified Biosensors Powered by Endogenous Molecules for Intracellular Fluorescence Imaging

Zhou,

Zhang,

Sun

et al. 2024

Anal. Chem.

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“…Similarly, based on the Exo III-assisted amplification, released trigger DNA repeatedly open oligo-2 and promote the generation of plentiful G-quadruplex DNA. N -Methylmesoporphyrin IX (NMM) is selected as the fluorochrome because its fluorescence intensity presented apparent enhancement, owing to the formation of the G-quadruplex-NMM complex [31, 32]. The proposed method displays high distinction efficiency towards Ag + against other environmentally relevant metal ions, and it is successfully applied to the detection of Ag + in real samples.…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Fluorescence Biosensor for Silver Ions (Ag⁺) Detection Based on C-Ag⁺-C Structure and Exonuclease III-Assisted Dual-Recycling Amplification

Yuan

2019

Journal of Analytical Methods in Chemistry

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A C-Ag+-C structure-based fluorescence biosensor with novel combination design of exonuclease III (Exo III) dual-recycling amplification is proposed for the application of silver ions (Ag+) detection. Since oligo-1 involves C-C mismatches, the presence of Ag+ can be captured to form C-Ag+-C base pairs, which results in a double-helix structure with a blunt terminus. The double-helix structure can be cleaved by EXO III to release short mononucleotide fragments (trigger DNA) and Ag+. Released Ag+ can form new bindings with oligo-1, and other trigger DNA can be produced in the digestion cycles. Hybridization with the signal DNA (oligo-2) transforms a trigger DNA into double-stranded DNA with blunt terminus which can be cleaved by Exo III to reproduce the trigger DNA and form guanine- (G-) quadruplex DNA. The trigger DNA returns free to the solution and hybridizes with another signal DNA, which realizes the dual-recycling amplification. The G-quadruplex DNA can be reported by N-methylmesoporphyrin IX (NMM), a specific G-quadruplex DNA fluorochrome. This method allows Ag+ to be determined in the 5 to 1500 pmol/L concentration range, with a 2 pmol/L detection limit, and it has been successfully applied to the detection of Ag+ in real samples.

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Binding induced strand displacement amplification for homogeneous protein assay

Cited by 16 publications

References 60 publications

Hybridization Cascade Plus Strand-Displacement Isothermal Amplification of RNA Target with Secondary Structure Motifs and Its Application for Detecting Dengue and Zika Viruses

Hybridization Cascade Plus Strand-Displacement Isothermal Amplification of RNA Target with Secondary Structure Motifs and Its Application for Detecting Dengue and Zika Viruses

Amplified Biosensors Powered by Endogenous Molecules for Intracellular Fluorescence Imaging

A Sensitive Fluorescence Biosensor for Silver Ions (Ag⁺) Detection Based on C-Ag⁺-C Structure and Exonuclease III-Assisted Dual-Recycling Amplification

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Binding induced strand displacement amplification for homogeneous protein assay

Cited by 16 publications

References 60 publications

Hybridization Cascade Plus Strand-Displacement Isothermal Amplification of RNA Target with Secondary Structure Motifs and Its Application for Detecting Dengue and Zika Viruses

Hybridization Cascade Plus Strand-Displacement Isothermal Amplification of RNA Target with Secondary Structure Motifs and Its Application for Detecting Dengue and Zika Viruses

Amplified Biosensors Powered by Endogenous Molecules for Intracellular Fluorescence Imaging

A Sensitive Fluorescence Biosensor for Silver Ions (Ag+) Detection Based on C-Ag+-C Structure and Exonuclease III-Assisted Dual-Recycling Amplification

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A Sensitive Fluorescence Biosensor for Silver Ions (Ag⁺) Detection Based on C-Ag⁺-C Structure and Exonuclease III-Assisted Dual-Recycling Amplification