We study the interaction of Λ−hyperon with proton and neutron inside a nucleus within the framework of relativistic mean field formalism. The single particle energy levels for some of the specific proton and neutron orbits are analyzed with the addition of Λ−successively. We found that the interaction of Λ with neutron is more stronger than proton.Hypernucleus provides an opportunity to enter into the strangeness world. After introducing the strangeness degree of freedom to bound nuclear system, the multibaryonic system avails Λ-nucleon (ΛN ) interaction in addition to nucleon-nucleon (NN) interaction. However, ΛN interaction is weaker than NN but it is imperative as well as important to describe the strange system. Many of the theoretical calculations have been made to give the importance of ΛN interaction and to facilitate the path toward multi-strange systems [1][2][3][4][5].The information about the hyperon-nucleon interaction especially Λp and Λn can be extracted from the hypernuclei. Due to zero isospin of Λ−hyperon, the one pion exchange is prohibited in ΛN interaction. Which means the ΛN interaction is governed by two pion exchange. In this way, ΛN − ΣN coupling plays a significant role to drive the ΛN interaction. The most interested mirror hypernuclei are 4 Λ H and 4 Λ He which reflect the difference in strength of Λp and Λn interactions. The ΛN interaction occurs via ΛN − ΣN coupling where, Λp couples to Σ + n and Λn is coupled with Σ − p [6]. Therefore, ΣN plays a role as an intermediate state to access the ΛN interaction. On the basis of this coupling, it is expected that the mass difference of Σ + − Σ − is responsible to make a difference in the strength of Λp and Λn interactions as discussed in Refs. [2,6,7]. The difference in strength of these interactions is a direct consequence of charge symmetry breaking which is observed in mirror hypernuclei [2,6,7]. It is well understood that any kinds of change take place in the interaction is directly reflected in nuclear potential as well as single-particle energy. Thus, it is very much interesting to analyze the single-particle energy or potential to study the net effect on interaction, either with the addition of Λ−hyperon or any other effects. In this work, we study the singleparticle energy as well as potentials of some medium and superheavy hypernuclei to demonstrate this mechanism by employing the relativistic mean field (RMF) formalism.Recently, the RMF theory is quite successful for studying the finite and infinite nuclear systems. Quite successful to study the equation of state (EOS) for normal as well as high dense neutron matter. Since neutron star is a compact object with nuclear density ρ = (8 − 10)ρ 0 , where ρ 0 is the nuclear matter density at saturation, so there must be the possibility of formation of strange baryon. In this context, addition of strangeness degree of freedom to RMF formalism is obvious for the suitable expansion of the model and this type of attempts have already been made [4,[8][9][10][11][12][13][14][15][16][17].The re...