Binding-Directed Energy Transfer of Conjugated Polymer Materials for Dual-Color Imaging of Cell Membrane

Cui, Qianling; Wang, Xiaoyu; Yang, Yu; Li, Shengliang; Li, Lidong; Wang, Shu

doi:10.1021/acs.chemmater.6b01424

Cited by 67 publications

(51 citation statements)

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“…Wang et al produced a photoactive polymer bearing imidazolium group with an oligo(ethylene glycol) moiety, allowing both electrostatic binding upon the cell membrane and water compatibility for cell imaging. 129 Charge-assisted water-solubility of CPPs could be achieved by acid treatment of azulene-based conjugated microporous polymer. 130 The protonation of a cyclopentadiene ring of azulene under acidic conditions led to the formation of a stable aromatic tropylium cation, providing hydrophilicity toward the polymer network for photocatalytic water treatment.…”

Section: View Article Onlinementioning

confidence: 99%

Designing conjugated porous polymers for visible light-driven photocatalytic chemical transformations

2020

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Section: View Article Onlinementioning

confidence: 99%

Designing conjugated porous polymers for visible light-driven photocatalytic chemical transformations

2020

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“…Conjugated polymers (CPs) possess good light‐harvesting activities and high quantum yields, which can enable efficient FRET . Recently, our group designed and synthesized a novel CP PFDBT‐BIMEG, as shown in Figure a. The poly(fluorene) (PF) and 1,4‐dithienylbenzothiadiazole (DBT, molar ratio is 5%) in the backbone served as a donor and acceptor pair, which led to intrachain FRET.…”

Section: Fret‐based Fluorescent Platformsmentioning

confidence: 99%

Section: Fret‐based Fluorescent Platformsmentioning

confidence: 99%

Fluorescent Platforms Based on Organic Molecules for Chemical and Biological Detection

Wang

Liu

Zhu

et al. 2018

Physica Rapid Research Ltrs

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Fluorescent platforms based on organic molecules have attracted great attention in the field of chemical and biological detection because of their high detection efficiency and sensitivity. Strategies in fluorescent platform design with controlled luminescence properties based on Förster resonance energy transfer, excimer/exciplex formation, and metal‐enhanced fluorescence mechanisms are reviewed herein. Attention is focused on the photophysical processes of fluorescent molecules in their interactions with other components as well as with the target. Color‐ and intensity‐tunable luminescence from energy or electron transfer and the electronic structures of fluorescent molecules are discussed. On the basis of the photophysical properties of fluorescent molecules, their responsive fluorescence signals, which reflect specific inter‐ and intra‐molecular interactions, are particularly effective for chemical and biological detection.

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“…Although highly hydrophobic fluorophores are efficient markers of membrane models such as liposomes, they generally fail in staining the cell PM of live cells as they tend to precipitate before reaching the membrane and to quickly cross the PM to stain inner membranes. 12, 17 Recent efforts have been made to develop specific and efficient PM probes for cell imaging with various fluorophores including chromone, 18–19 Nile Red, 20 styryl pyridinium, 21 BODIPY, 22–23 perylene, 24 oligothiophene, 25–26 conjugated polymers, 27 or FITC-labelled chitosan. 28–29 From the rapid development of multicolor imaging arose a demand for red-shifted fluorescent markers.…”

Section: Introductionmentioning

confidence: 99%

MemBright: a Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience

Collot

Ashokkumar

Anton

et al. 2018

Preprint

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The proper staining of the plasma membrane (PM) is critical in bioimaging as it 16 delimits the cell. Herein, we developed MemBright: a family of six cyanine--based fluorescent 17 turn--on PM probes that emit from orange to near--infrared when reaching the PM, and 18 enable homogeneous and selective PM staining with excellent contrast in mono and two--19 photon microscopy. These probes are compatible with long--term live cell imaging and 20 immunostaining. Moreover, MemBright label neurons in a brighter manner than 21 surrounding cells allowing identification of neurons in acute brain tissue section and 22 neuromuscular--junctions without any use of transfection or transgenic animals. At last, 23MemBright were used in super--resolution imaging to unravel the dendritic spines' neck. 3D 24 multicolor dSTORM in combination with immunostaining revealed en--passant synapse 25 displaying endogenous glutamate receptors clustered at the axonal--dendritic contact site. 26MemBright probes thus constitute a universal toolkit for cell biology and neuroscience 27 biomembrane imaging with a variety of microscopy techniques. 29 30Visualizing plasma membrane is particularly important in neuroscience, because it enables 1 visualization neuronal organization and membrane trafficking involved in the synaptic 2 transmission. 3 Recent years have seen a tremendous expansion of fluorescence imaging 3 techniques and tools for cellular research. In addition to genetically encoded fluorescent 4 proteins, a number of molecular probes for monitoring cellular events have been 5 developed. 4, 5 The key challenge in cellular imaging is to stain cell compartments with high 6 specificity and persistence. Although numerous efficient molecular probes have been 7 designed to selectively stain specific cellular structures including mitochondria, 6 lipid 8 droplets, 7 endoplasmic reticulum, 8 nucleus 9 and lysosomes, 10--11 there is still a demand for 9 efficient and bright fluorescent PM markers. 12 Fluorescently labelled lectins notably wheat 10 germ agglutinin (WGA) and concanavalin A 9 are popular fluorescent membrane probes. 11Despite their efficiency and ease of use, these probes are expensive and much larger than 12 molecular probes. The small size of the latter, comparable to lipids, allows their precise 13 location in the lipid bilayer, which is of key importance for studies of the lateral lipid 14 organization of biomembranes (lipid rafts), 12, 13 FRET with membrane proteins 14 and super--15 resolution imaging. 15--16 Therefore, molecular probes are an interesting alternative to 16 protein--based membrane markers. Although highly hydrophobic fluorophores are efficient 17 markers of membrane models such as liposomes, they generally fail in staining the cell PM of 18 live cells as they tend to precipitate before reaching the membrane and to quickly cross the 19 PM to stain inner membranes. 12, 17 Recent efforts have been made to develop specific and 20 efficient PM probes for cell imaging with various fluorophores including chromone, 18--19 ...

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Binding-Directed Energy Transfer of Conjugated Polymer Materials for Dual-Color Imaging of Cell Membrane

Cited by 67 publications

References 50 publications

Designing conjugated porous polymers for visible light-driven photocatalytic chemical transformations

Designing conjugated porous polymers for visible light-driven photocatalytic chemical transformations

Fluorescent Platforms Based on Organic Molecules for Chemical and Biological Detection

MemBright: a Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience

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