Binding characteristics of Escherichia coli adhesins in human urinary bladder

Virkola, Ritva; Westerlund, Benita; Holthöfer, Harry; Parkkinen, Jaakko; Kekomäki, M; Korhonen, Timo

doi:10.1128/iai.56.10.2615-2622.1988

Cited by 122 publications

(38 citation statements)

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“…The binding of 075X E. coli strains to human and canine kidney structures has been examined (15,26,38,40), and recently, the Dr blood group antigen of the human IFC blood group complex has been identified as the erythrocyte receptor (26). The adhesins expressed by E. coli 950 and 5026 were identified as Dr hemagglutinins because both strains failed to agglutinate Dr (a-) erythrocytes which lack the Dr blood group antigen (26).…”

Section: Discussionmentioning

confidence: 99%

“…One group of X adhesins is frequently associated with E. coli strains belonging to serogroup 075 and have been called 075X adhesins. They have been shown to bind preferentially to basement membranes of human and canine kidneys, Bowman's capsule, and, to a lesser extent, to the bladder epithelium (15,26,38,40). Several 075X+ E. coli strains have also been shown to bind the Dr blood group antigen of the human IFC blood group complex and have, accordingly, been renamed Dr hemagglutinins (26).…”

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confidence: 99%

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Purification and characterization of the Dr hemagglutinins expressed by two uropathogenic Escherichia coli strains

1990

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The fibrillar Dr hemagglutinins expressed by two uropathogenic Escherichia coli isolates were mechanically sheared from whole cels and subsequently purified by using anion-exchange high-pressure liquid chromatography. The isolated hemagglutinins were proteins with apparent subunit molecular masses of 14,500 daltons by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric points of 5.4 in denaturing isoelectric focusing gels. The two proteins were serologically related to each other but distinct from P fimbriae, as assessed by bacterial agglutination and immunoblotting. The amino acid compositions of the two hemagglutinins were highly similar both to each other and to other Dr hemagglutinins. N-terminal amino acid sequencing of the major hemagglutinin subunit proteins demonstrated homology with afimbrial E. coli adhesins. 695 on July 7, 2020 by guest http://iai.asm.org/ Downloaded from

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Section: Discussionmentioning

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Purification and characterization of the Dr hemagglutinins expressed by two uropathogenic Escherichia coli strains

1990

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“…Although associated primarily with the pathogenesis of lower UTI (106), type 1 pili may also be involved in the pathogenesis of pyelonephritis (78,141). In this setting, these structures have been shown to bind effectively to proximal tubulus cells (248). Type 1 fimbriae are also capable of binding to soluble, mannosyl-containing glycoproteins in urine, such as the Tamm-Horsfall protein (199), or in saliva (13).…”

Section: Pili (Fimbriae)mentioning

confidence: 99%

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

1998

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SUMMARY Bacteria belonging to the genus Klebsiella frequently cause human nosocomial infections. In particular, the medically most important Klebsiella species, Klebsiella pneumoniae, accounts for a significant proportion of hospital-acquired urinary tract infections, pneumonia, septicemias, and soft tissue infections. The principal pathogenic reservoirs for transmission of Klebsiella are the gastrointestinal tract and the hands of hospital personnel. Because of their ability to spread rapidly in the hospital environment, these bacteria tend to cause nosocomial outbreaks. Hospital outbreaks of multidrug-resistant Klebsiella spp., especially those in neonatal wards, are often caused by new types of strains, the so-called extended-spectrum-β-lactamase (ESBL) producers. The incidence of ESBL-producing strains among clinical Klebsiella isolates has been steadily increasing over the past years. The resulting limitations on the therapeutic options demand new measures for the management of Klebsiella hospital infections. While the different typing methods are useful epidemiological tools for infection control, recent findings about Klebsiella virulence factors have provided new insights into the pathogenic strategies of these bacteria. Klebsiella pathogenicity factors such as capsules or lipopolysaccharides are presently considered to be promising candidates for vaccination efforts that may serve as immunological infection control measures.

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“…Another adhesin, the type I fimbrial adhesin, is produced by pathogenic and nonpathogenic strains and is able to bind to a-D-mannose-containing receptors (47). Fimbriae of serotype FIC are unable to agglutinate erythrocytes but do seem to interact with cells of the human urinary tract (56,68,69).E. coli strains may be subdivided into clones by electrophoretic typing of alloenzymes (4) and by outer membrane protein (OMP) profiles (3).…”

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Virulence patterns and long-range genetic mapping of extraintestinal Escherichia coli K1, K5, and K100 isolates: use of pulsed-field gel electrophoresis

et al. 1991

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A total of 127 extraintestinal Escherichia coli strains of the capsule serotypes Kl, K5, and K100 from human and animal sources were analyzed for DNA sequences specific for the genes for various adhesins (P fimbriae [pap] and P-related sequences [prs], S fimbriae [sfa]IFIC fimbriae [foc], and type I fimbriae [fim]), aerobactin (aer), and hemolysin (hly). The expression of corresponding virulence factors was also tested. Twenty-four selected strains were analyzed by long-range DNA mapping to evaluate their genetic relationships. DNA sequences for the adhesins were often found in strains not expressing them, while strains with hemolysin and aerobactin genes usually did express them. Different isolates of the same serotype often expressed different virulence patterns. The use of virulence-associated gene probes for Southern hybridization with genomic DNA fragments separated by pulsed-field gel electrophoresis revealed that a highly heterogeneous restriction fragment length and hybridization pattern existed even within strains of the same serotype. Long-range DNA mapping is therefore useful for the evaluation of genetic relatedness among individual isolates and facilitates the performance of precise molecular epidemiology.Escherichia coli strains of the Ki capsular serotype represent 80% of all E. coli strains isolated from cases of newborn meningitis and sepsis in humans (24,57,63). Moreover, Kl strains and isolates of other serotypes, including 018:K5 and 075:K100, are able to cause urinary tract infection, and Kl E. coli isolates are also often found to be the causative agent of systemic infections in animals (3,48,64,70,71).It has been clear for several years that the capsular antigens, especially Kl and certain types of 0 antigen, are strongly associated with such extraintestinal infections (4, 33, 60, 61, 64), as are specific fimbrial adhesins (18,42,45,46), hemolysin (Hly) (20,22,23), and aerobactin (Aer) (11,28,37). Extraintestinal E. coli isolates may carry different types of fimbrial adhesins which can be distinguished serologically (47) and can show different binding properties. P fimbriae (also termed Pap pili [45]) are associated with pyelonephritis and recognize an Ot-D-galactosyl-(1-4)-p-galactose receptor (29, 66). P-related sequences (Prs) mediate binding to galactosyl-N-acetyl-(ox1-3)-galactosyl-N-acetyl residues (40). The S-fimbrial adhesin (Sfa) interacts with a-sialyl(2-3p)-D-galactose receptor molecules (32,54). Another adhesin, the type I fimbrial adhesin, is produced by pathogenic and nonpathogenic strains and is able to bind to a-D-mannose-containing receptors (47). Fimbriae of serotype FIC are unable to agglutinate erythrocytes but do seem to interact with cells of the human urinary tract (56,68,69).E. coli strains may be subdivided into clones by electrophoretic typing of alloenzymes (4) and by outer membrane protein (OMP) profiles (3). In some cases, the clones have a characteristic serotype based on the 0 lipopolysaccharide and K capsular antigens (4). Attempts have been made to * Corres...

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Binding characteristics of Escherichia coli adhesins in human urinary bladder

Abstract: We studied domains in the human bladder that acted as receptors for Escherichia coli P, S, type 1, type 1C, and 075X fimbriae or adhesin and domains in the human kidneys that were receptors for E. coli type 1C

Cited by 122 publications

References 53 publications

Purification and characterization of the Dr hemagglutinins expressed by two uropathogenic Escherichia coli strains

Purification and characterization of the Dr hemagglutinins expressed by two uropathogenic Escherichia coli strains

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

Virulence patterns and long-range genetic mapping of extraintestinal Escherichia coli K1, K5, and K100 isolates: use of pulsed-field gel electrophoresis

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