Binding capacity of molecularly imprinted polymers and their nonimprinted analogs

Dorkó, Zsanett; Szakolczai, Anett; Verbić, Tatjana Ž.; Horvai, George

doi:10.1002/jssc.201500874

Cited by 17 publications

(19 citation statements)

References 34 publications

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“…Isotherm studies at such high loadings appear to be rare although they might prove to be useful. For example it has been found [66] that a propranolol imprinted MIP can actually bind propranolol equivalent to more than 25% of its COOH content, while the binding capacity of a very similarly prepared MIP had been estimated from the binding isotherm (measured at lower concentrations) as merely 4% of its COOH content [18]. (The somewhat different experimental conditions between these experiments do not seem to support so much discrepancy.)…”

Section: Using the Log-log Isotherms To Understand And Design Practicmentioning

confidence: 99%

Isotherm charts for material selection and method development with molecularly imprinted polymers and other sorbents

Dorkó

Tamás

Horvai

2017

Talanta

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A simple and efficient method is presented for assessing molecularly imprinted polymers (MIP) and other sorbents from the point of view of practical applications. The adsorption isotherms of the compounds, which need to be separated or detected in an application, are constructed from a small number of measured points on a log-log chart and then are compared graphically. Despite its simplicity and robustness this method reveals the information needed for optimal selection between MIPs and alternative sorbents. The design of separation or detection methods with MIPs is also supported by the proposed graphical isotherm comparison. Many experimental isotherms are presented supporting the proposed method.

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Section: Using the Log-log Isotherms To Understand And Design Practicmentioning

confidence: 99%

Isotherm charts for material selection and method development with molecularly imprinted polymers and other sorbents

Dorkó

Tamás

Horvai

2017

Talanta

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“…The results of Dorkó et al. have shown that in a variety of differently prepared MAA‐based molecularly imprinted and nonimprinted polymers, 80–90% of carboxylic groups retained in the polymer can be accessed by strong bases, and essentially the same amount can be used for adsorption of weak bases. However, this high level of adsorption can only be achieved if the adsorbed weak base is strong enough, if the polymer is sufficiently elastic and if the solvent does not compete too strongly for the binding sites.…”

Section: Resultsmentioning

confidence: 99%

Separation of 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane and 1,3,5-trinitro-1,3,5- triazacyclohexane by molecularly imprinted solid-phase extraction

et al. 2017

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Synthesis of 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane and 1,3,5-trinitro-1,3,5-triazacyclohexane by the Bachmann process leads to a mixture of both. The separation of 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane and 1,3,5-trinitro-1,3,5-triazacyclohexane from their mixture is difficult because the sizes and physical properties of these homologous compounds are similar. For this purpose, seven molecularly imprinted polymers have been synthesized for each explosive, and a selective solid-phase extraction procedure has been developed. A molecularly imprinted polymer, synthesized with 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane as the template, methacrylic acid as the monomer and trimethylolpropane trimethacrylate as the cross-linking agent in a molar ratio of 1:8:8 showed the best separation capability. A packed cartridge containing this polymer can be reused for 23 solid-phase extraction cycles without repacking, and the total separation capability toward 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane reached 6.81 mg per gram of polymer. 1,3,5-Trinitro-1,3,5-triazacyclohexane was not detected in the separated 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane by high-performance liquid chromatography and vice versa. This newly developed method had the advantages of high recovery (100%) and purity, environmental friendliness, and room temperature operability. This study showed that some molecularly imprinted polymers that cannot absorb target analytes well in the solvent in which the polymers were polymerized might have high-binding capacity for the analytes and show imprinting effects in other solvents.

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“…The LC-PDA analysis revealed peaks at 17.59, 21.24, 21.83, 40.66, 43.06, and 51.72 min (Fig. 4), which indicated the binding affinity to the receptor LDH [31].…”

Section: Evaluation Of Lactate Dehydrogenase Inhibitory Activity and mentioning

confidence: 97%

Ultrafiltration‐LC–MS combined with semi‐preparative HPLC for the simultaneous screening and isolation of lactate dehydrogenase inhibitors from Belamcanda chinensis

Tang

et al. 2016

J of Separation Science

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Stroke represents the fourth leading cause of death in the USA and the second leading cause of death worldwide. Lactate dehydrogenase inhibitors are widely used in the treatment of ischemic stroke and natural products are considered a promising source of novel lactate dehydrogenase inhibitors. In this study, we used PC12 cells to determine the protective effect of extracts from the herb Belamcanda chinensis following toxic challenge. Using ultrafiltration high-performance liquid chromatography coupled with photo-diode array detection and electrospray ionization mass spectrometry, we screened and identified isoflavonoids from Belamcanda chinensis extracts. Semi-preparative high-performance liquid chromatography was then applied to separate and isolate the active constituents. Using these methods, we identified six major compounds in Belamcanda chinensis as lactate dehydrogenase inhibitors: tectoridin, iristectorin A, iridin, tectorigenin, irigenin, and irisflorentin, which were then isolated to >92% purity. This is the first report that Belamcanda chinensis extracts contain potent lactate dehydrogenase inhibitors. Our results demonstrate that the systematic isolation of bioactive components from Belamcanda chinensis guided by ultrafiltration high-performance liquid chromatography coupled with photo-diode array detection and electrospray ionization mass spectrometry represents a feasible and efficient technique that could be extended for the identification and isolation of other enzyme inhibitors.

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Binding capacity of molecularly imprinted polymers and their nonimprinted analogs

Cited by 17 publications

References 34 publications

Isotherm charts for material selection and method development with molecularly imprinted polymers and other sorbents

Isotherm charts for material selection and method development with molecularly imprinted polymers and other sorbents

Separation of 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane and 1,3,5-trinitro-1,3,5- triazacyclohexane by molecularly imprinted solid-phase extraction

Ultrafiltration‐LC–MS combined with semi‐preparative HPLC for the simultaneous screening and isolation of lactate dehydrogenase inhibitors from Belamcanda chinensis

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