Binding between calcium ions and chondroitin sulfate chains of salmon nasal cartilage glycosaminoglycan

Uchisawa, Hidemitsu; Okuzaki, Bun-ichi; Ichita, Junji; Matsue, Hiroyuki

doi:10.1016/s0531-5131(01)00458-7

Cited by 34 publications

(29 citation statements)

References 5 publications

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“…Large number of bands appeared in the HAp spectra (3571, 1459, 1415, 1089, 1050, 962, 633, 603 and 571 cm −1 ) that were similar to HAp reference spectrum close to earlier data [29,40]. IR Spectra of CS revealed the presence of peak at 823 cm −1 which was used to identify CS as per the previous report of Uchisawa et al [41]. The infrared spectra of AP exhibited almost identical bands with starch.…”

Section: Stretching Frequency Of Scaffoldssupporting

confidence: 85%

Chitosan–amylopectin/hydroxyapatite and chitosan–chondroitin sulphate/hydroxyapatite composite scaffolds for bone tissue engineering

Venkatesan

Pallela

Bhatnagar

et al. 2012

International Journal of Biological Macromolecules

137

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Section: Stretching Frequency Of Scaffoldssupporting

confidence: 85%

Chitosan–amylopectin/hydroxyapatite and chitosan–chondroitin sulphate/hydroxyapatite composite scaffolds for bone tissue engineering

Venkatesan

Pallela

Bhatnagar

et al. 2012

International Journal of Biological Macromolecules

137

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“…Our previous work [14, 15] described in detail the building of the PCR algorithm by PerkinElmer software, Spectrum Quart+. The standard and target spectra in the range from 960 to 4000 cm −1 were imported into the PCR calculations, since the chondroitin 6-sulfate (CS6) as major GAG in PG was much more preponderant than the CS4 [23–25] and both CS4 and CS6 showed the identical IR spectra within the range of 4000-960 cm −1 [26, 27]. …”

Section: Methodsmentioning

confidence: 99%

Proteoglycan concentrations in healthy and diseased articular cartilage by Fourier transform infrared imaging and principal component regression

Yin

Xia

2014

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Fourier transform infrared imaging (FTIRI) combining with principal component regression (PCR) analysis were used to determine the reduction of proteoglycan (PG) in articular cartilage after the transection of the anterior cruciate ligament (ACL). A number of canine knee cartilage sections were harvested from the meniscus-covered and meniscus-uncovered medial tibial locations from the control joints, the ACL joints at three time points after the surgery, and their contralateral joints. The PG loss in the ACL cartilage was related positively to the durations after the surgery. The PG loss in the contralateral knees was less than that of the ACL knees. The PG loss in the meniscus-covered cartilage was less than that of the meniscus-uncovered tissue in both ACL and contralateral knees. The quantitative mapping of PG loss could monitor the disease progression and repair processes in arthritis.

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“…The sGAGs, including C4S, and their associated proteoglycans are components of the cell surface and the extracellular matrix (ECM). They are strongly anionic due to their multiple sulfate groups and bind with cations or cationic proteins [12,13]. We suggest that there may be increased demand for cations following reduction of sulfatase activity, in order to combine with the increased sulfate groups of C4S on the cell surface.…”

Section: Discussionmentioning

confidence: 93%

Arylsulfatase B regulates colonic epithelial cell migration by effects on MMP9 expression and RhoA activation

Bhattacharyya

Tobacman

2009

Clin Exp Metastasis

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Arylsulfatase B (ASB; N-acetylgalactosamine-4-sulfatase; 4-sulfatase; ARSB) is the enzyme that removes 4-sulfate groups from N-acetylgalactosamine 4-sulfate, which combines with glucuronate to form the disaccharide unit of chondroitin-4-sulfate (C4S). In this study, we report how variation in expression of ASB affected the migration of human colonic epithelial cells. In the T84 cell line, derived from lung metastasis of malignant colonic epithelial cells, the activity of ASB, as well as steroid sulfatase, arylsulfatase A, and galactose-6-sulfatase, were significantly less than in normal, primary colonic epithelial cells and in the NCM460 cell line which was derived from normal colonocytes. In the T84 cells, matrix metalloproteinase 9 (MMP9), activated RhoA, and cell migration, as well as C4S content, were significantly more than in the NCM460 cells. Silencing and overexpression of ASB had inverse effects on MMP9, activated RhoA, and cell migration, as well as the C4S content, in the NCM460 and T84 cells. When ASB expression was silenced by siRNA in the NCM460 cells, MMP9 secretion increased to over 3 times the basal level, activated RhoA increased * 85%, and cell migration increased * 52%. Following overexpression of ASB, MMP9 declined 51%, activated RhoA declined * 51%, and cell migration decreased * 37%. These findings demonstrate marked effects of ASB expression on the migratory activity of colonic epithelial cells, activated RhoA, and MMP9, and suggest a potential vital role of ASB, due to its impact on chondroitin sulfation, on determination of the invasive phenotype of colonic epithelial cells.

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Binding between calcium ions and chondroitin sulfate chains of salmon nasal cartilage glycosaminoglycan

Cited by 34 publications

References 5 publications

Chitosan–amylopectin/hydroxyapatite and chitosan–chondroitin sulphate/hydroxyapatite composite scaffolds for bone tissue engineering

Chitosan–amylopectin/hydroxyapatite and chitosan–chondroitin sulphate/hydroxyapatite composite scaffolds for bone tissue engineering

Proteoglycan concentrations in healthy and diseased articular cartilage by Fourier transform infrared imaging and principal component regression

Arylsulfatase B regulates colonic epithelial cell migration by effects on MMP9 expression and RhoA activation

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