Binding and storage of heme by vitellin from the cattle tick, Boophilus microplus

Logullo, Carlos; Moraes, João Antônio de; Dansa-Petretski, Marı́lvia; Vaz, Itabajara S.; Masuda, Aoi; Sorgine, Marcos Henrique Ferreira; Braz, Glória R.C.; Masuda, Hatisaburo; Oliveira, Pedro L.

doi:10.1016/s0965-1748(02)00162-5

Cited by 61 publications

(56 citation statements)

References 27 publications

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“…The observation that eggs accumulate a great amount of heme after vitellin -VT processing/degradation (vitellin is the main protein of reserve of eggs) (LOGULLO et al, 2002) supports the hypothesis that the degradation of vitellin during the embryonic development is a source of oxidative stress. Therefore, a critical adaptation is necessary, which may prevent excessive reactive oxygen species production during several physiologic conditions.…”

Section: Tick Heme-binding Aspartic Proteinase -Thapsupporting

confidence: 52%

“…The increase in egg free heme content would generate a feedback inhibition in VT degradation. The idea that VT degradation is a process related to the regulation of redox balance and to the use of heme by the embryo was reinforced by the demonstration that VT is also a heme binding protein and that it inhibits lipid peroxidation induced by heme (LOGULLO et al, 2002).…”

Section: Tick Heme-binding Aspartic Proteinase -Thapmentioning

confidence: 99%

“…It was observed that total egg protein remains stable during embryogenesis (CAMPOS et al, 2006), while VT content suffers a variation (CANAL et al, 1995;LOGULLO et al, 2002). Thus, VT is used for the supply of the amino acids required to synthesize new proteins.…”

Section: Boophilus Yolk Pro-cathepsin and Tick Vitellin-degrading Enzymementioning

confidence: 99%

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New approaches toward anti-Rhipicephalus (Boophilus) microplus tick vaccine

Parizi¹,

Pohl²,

Masuda³

et al. 2009

RBPV

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The tick Rhipicephalus (Boophilus) microplus (formerly Boophilus microplus) is the major ectoparasite affecting livestock in America, Asia, Africa, and Oceania. Conventional tick control is based on the use of acaricides but immunization of bovines with tick gut proteins induces only a partial protective immune response. Based on this information, distinct research groups have explored the possibility of protecting the animals by inducing an immune response against other tick proteins. However, the antigens so far described do not induce the necessary protection for suppressing the use of acaricides. Currently, several groups are engaged in identifying new tick proteins to be used as targets for the development of new vaccines. This approach focuses on the enhancement of the immunogenicity of antigens already tested by incorporating new adjuvants or formulations and by searching for new antigens. This paper reviews the work done by Brazilian researchers to develop a vaccine against this tick.Keywords: Boophilus microplus, Rhipicephalus microplus, vaccine, tick, control. ResumoO carrapato Rhipicephalus (Boophilus) microplus (anteriormente Boophilus microplus) é o principal ectoparasita que afeta bovinos na América, Ásia, África e Oceania e o seu controle é tradicionalmente realizado através do uso de acaricidas. Experimentos de imunização com proteínas do carrapato mostram que a resposta imune desenvolvida pelos bovinos vacinados protege, em parte, os animais do parasitismo. Baseado nessas observações, vários grupos de pesquisa exploram a possibilidade de proteger os animais pela indução de uma resposta imune contra proteínas do carrapato. Entretanto, os antígenos já caracterizados não asseguram o grau de proteção necessário para suprimir o uso de acaricidas. Portanto, esses grupos de pesquisa estão engajados na tentativa de identificar novas proteínas que possam ser utilizadas para o desenvolvimento de novas vacinas, as quais possam induzir maior imunogenicidade de que os antígenos já testados, através do uso de novas formulações e/ou pela incorporação de adjuvantes. O presente artigo apresenta uma revisão da literatura sobre os resultados obtidos por pesquisadores brasileiros no desenvolvimento de vacinas contra o carrapato.

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Section: Tick Heme-binding Aspartic Proteinase -Thapsupporting

confidence: 52%

Section: Tick Heme-binding Aspartic Proteinase -Thapmentioning

confidence: 99%

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New approaches toward anti-Rhipicephalus (Boophilus) microplus tick vaccine

Parizi¹,

Pohl²,

Masuda³

et al. 2009

RBPV

View full text Add to dashboard Cite

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“…Because simple binding of heme to a protein may be not sufficient to prevent heme-induced formation of reactive oxygen species (16,50), which, in turn may lead to non-enzymatic degradation of heme (42), we investigated the effect of sMF6p/ FhHDM-1⅐hemin interaction on hemin reactivity. Interestingly, sMF6p/FhHDM-1 completely prevented peroxidative destruction of hemin by H 2 O 2 , which supports its possible function in preserving heme integrity against potential oxidative species in the environment and enabling its use as a source of heme for other heme proteins.…”

Section: Discussionmentioning

confidence: 99%

The MF6p/FhHDM-1 Major Antigen Secreted by the Trematode Parasite Fasciola hepatica Is a Heme-binding Protein

Martínez-Sernández

Mezo²,

González-Warleta³

et al. 2014

Journal of Biological Chemistry

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Background:The mechanisms involved in heme handling in trematodes are poorly understood. Results: The biochemical and functional characteristics of a new family of small proteins (MF6p/FhHDM-1) secreted by Fasciola and other trematodes are reported. Conclusion:The Fasciola MF6p/FhHDM-1 major antigen is a heme-binding protein. Significance:Our results provide new insights into the biology of hematophagous trematodes.

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“…These results are in agreement with the observed intense concentration of haem at the basal surface of the digest cell during the initial days ABM, which coincide with the period of most intense vitellogenic oocyte growth, suggesting that by this time the absorption pathway seems to predominate over hemosome formation (Fig.·1A,B). This phenomenon is dependent on the accumulation of vitellin, the major yolk protein of oviparous animals (Sappington and Raikhel, 1998), which in ticks is a haemoprotein (Rosell and Coons, 1991) that functions as a haem reservoir to support embryo development (Logullo et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

A new intracellular pathway of haem detoxification in the midgut of the cattle tick Boophilus microplus: aggregation inside a specialized organelle, the hemosome

Lara

Lins

Paiva-Silva

et al. 2003

Journal of Experimental Biology

105

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The hard tick Boophilus microplus ingests large volumes of cattle blood, as much as 100 times its own mass before feeding. Huge amounts of haem are produced during haemoglobin digestion, which takes place inside acidic lysosomal-type vacuoles of the digest cells of the midgut. Haem is a promoter of free radical formation, so haemoglobin digestion poses an intense oxidative challenge to this animal.In the present study we followed the fate of the haem derived from haemoglobin hydrolysis in the digest cells of the midgut of fully engorged tick females. The tick does not synthesize haem, so during the initial phase of blood digestion, absorption is the major route taken by the haem, which is transferred from the digest cells to the tick haemocoel. After this absorptive period of a few days, most of the haem produced upon haemoglobin degradation is accumulated in the interior of a specialized, membrane-delimited, organelle of the digest cell, herein called hemosome. Haem accounts for 90% of the hemosome mass and is concentrated in the core of this structure, appearing as a compact, non-crystalline aggregate of iron protoporphyrin IX without covalent modifications. The unusual FTIR spectrum of this aggregate suggests that lateral propionate chains are involved in the association of haem molecules with other components of the hemosome, which it is proposed is a major haem detoxification mechanism in this bloodsucking arthropod.

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Binding and storage of heme by vitellin from the cattle tick, Boophilus microplus

Cited by 61 publications

References 27 publications

New approaches toward anti-Rhipicephalus (Boophilus) microplus tick vaccine

New approaches toward anti-Rhipicephalus (Boophilus) microplus tick vaccine

The MF6p/FhHDM-1 Major Antigen Secreted by the Trematode Parasite Fasciola hepatica Is a Heme-binding Protein

A new intracellular pathway of haem detoxification in the midgut of the cattle tick Boophilus microplus: aggregation inside a specialized organelle, the hemosome

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