“…REES is indeed observed when the above criteria are satisfied (Ladokhin et al, 1991;Mukherjee, 1993, 1999a,b;Chattopadhyay and Rukmini, 1993;Hutterer et al, 1996;Chattopadhyay et al, 1997;Ghosh et al, 1997;Santos et al, 1998;MacPhee et al, 1999;Raja et al, 1999;Granjon et al, 2001). In addition, it is preferable that the membrane-embedded molecule has only one fluorescent group which has a unique location in the membrane and not a distribution of locations.…”