Binding and inhibitory effect of the food colorants Sunset Yellow and Ponceau 4R on amyloid fibrillation of lysozyme

Abstract

Amyloid fibrillogenesis of proteins is known to be the root cause of a large number of diseases like Parkinson's, Alzheimer's, and Huntington's disease, spongiform encephalopathy, amyloid polyneuropathy, type-II diabetes, etc.

Impact of chromate and dichromate on lysozyme stability: A spectroscopic and molecular docking investigation

“…Molecular docking is a useful method to evaluate the interactions of the ligand molecule with protein. [ 43,44 ] Thus, molecular docking studies were performed for chromate and dichromate with Lyz which provided evidence for their interactions on the Lyz. The binding free energy of dichromate and chromate to Lyz was identified to be −65.5 and −45.9 kJ mol −1 , respectively.…”

Using Small-Molecule Probes to Investigate Aggregation of Sunset Yellow FCF: What are the Concentration Limits?

“…26 These investigations demonstrated that sunset yellow binds to exposed hydrophobic surface such as solvent-accessible tryptophan residues. 26 Related to this, the binding of sunset yellow to pepsin has implications for its use as a food additive. A combination of fluorescence and molecular dynamic simulations reveals that sunset yellow causes an inhibition of pepsin, binding to a cluster of tyrosine and serine residues in the active site.…”

“…The interaction of dye molecules with larger structures is of wide interest. Sunset yellow interactions with amyloid fibril aggregates of lysozyme have been studied using a range of biophysical techniques, including transmission electron microscopy and various fluorescence assays . These investigations demonstrated that sunset yellow binds to exposed hydrophobic surface such as solvent-accessible tryptophan residues .…”

“…Sunset yellow interactions with amyloid fibril aggregates of lysozyme have been studied using a range of biophysical techniques, including transmission electron microscopy and various fluorescence assays . These investigations demonstrated that sunset yellow binds to exposed hydrophobic surface such as solvent-accessible tryptophan residues . Related to this, the binding of sunset yellow to pepsin has implications for its use as a food additive.…”

Sunset Yellow Dye Induces Amorphous Aggregation in β-Lactoglobulin at Acidic pH: A Multi-Techniques Approach

“…Carmoisine is an azo dye that binds with hen egg white lysozyme and shows an inhibitory effect on amyloid fibrillation in lysozyme [ 17 ]. Sunset Yellow (SY) and Ponceau 4R have also interacted with lysozyme at acidic pH and inhibiting temperature-induced amyloid fibrillation [ 18 ]. Keeping this in mind, we have evaluated the mechanism of interaction and amyloid-inducing property of SY on Beta-lactoglobulin (BLG) protein at acidic pH.…”