Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)

Davolos, Camila Chiaradia; Hernández‐Martínez, Patricia; Crialesi-Legori, Paula Cristina Brunini; Desidério, Janete Apparecida; Ferré, Juan; Escriche, Baltasar; Lemos, Manoel Victor Franco

doi:10.1016/j.jip.2015.01.013

Cited by 9 publications

(12 citation statements)

References 25 publications

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“…These bacteria present high genetic diversity and agricultural relevance (McSpadden Gardener, 2004), mainly for presenting nitrogenase activity in some species, such as B. subtilis, B. pumilus, and B. megaterium (Xie et al, 1998). A previous study has reported that many species of Bacillus can contribute significantly to the plant growth (Davolos et al, 2015). Recently, Zhang et al (2015) inoculated Bacillus megaterium in rice and observed a great promotion of growth, Mn uptake, and photosynthetic efficiency.…”

Section: Resultsmentioning

confidence: 99%

Diversity of plant growth-promoting bacteria associated with sugarcane

Antunes¹,

Lyra²,

Ollero³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. The sugarcane (Saccharum spp) presents economic importance, mainly for tropical regions, being an important Brazilian commodity. However, this crop is strongly dependent on fertilizers, mainly nitrogen (N). This study assessed the plant growth-promoting bacteria (PGPB) associated with sugarcane that could be used as a potential inoculant to the crop. We evaluated the genetic diversity of PGPB in the plant tissue of sugarcane varieties (RB 867515, RB 1011, and RB 92579). The primer BOX-A1R was used to differentiate the similar isolated and further sequencing 16S rRNA ribosomal gene. The 16S rRNA gene showed the presence of seven different genera distributed into four groups, the genus Bacillus, followed by Paenibacillus (20%), Burkholderia (14%), Herbaspirillum (6%), Pseudomonas (6%), Methylobacterium (6%), and Brevibacillus (3%). The molecular characterization of endophytic isolates from sugarcane revealed a diversity of bacteria colonizing this plant, with a possible biotechnological potential to be used as inoculant and biofertilizers.

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Section: Resultsmentioning

confidence: 99%

Diversity of plant growth-promoting bacteria associated with sugarcane

Antunes¹,

Lyra²,

Ollero³

et al. 2017

Genet. Mol. Res.

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“…Os resultados das competições heterólogas revelaram o compartilhamento do mesmo receptor entre as proteínas Cry1Aa, Cry1Ab e Cry1Ac (Figura 2). A proteína não marcada ligou-se aos receptores quando foi colocada em excesso, numa concentração de 1.000 x. Hernández-Rodríguez & Juan Ferré (2005) (Ballester et al, 1999;Karim et al, 2000;Jurat-Fuentes & Adang, 2001;Hernández-Rodríguez et al, 2013;Davolos et al, 2015). Esse compartilhamento pode estar relacionado a similaridades na sequência de aminoácidos, nas regiões II e III das proteínas Cry1Aa, Cry1Ab e Cry1Ac, visto que são regiões fundamentais e específicas para o modo de ligação dessas proteínas (Schnepf et al, 1998;Pardo-López et al, 2013).…”

Section: Methodsunclassified

Toxicidade e capacidade de ligação de proteínas Cry1 a receptores intestinais de Helicoverpa armigera (Lepidoptera: Noctuidae)

Sebastião

Lemes

Figueiredo

et al. 2015

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a toxicidade e a capacidade de ligação das proteínas Cry1Aa, Cry1Ab, Cry1Ac e Cry1Ca, de Bacillus thuringiensis, a receptores intestinais de Helicoverpa armigera. Realizou-se análise de ligação das proteínas ativadas às vesículas de membrana da microvilosidade apical (VMMA) do intestino médio de H. armigera, além de ensaios de competição heteróloga para avaliar sua capacidade de ligação. Cry1Ac destacou-se como a proteína mais tóxica, seguida por Cry1Ab e Cry1Aa. A proteína Cry1Ca não foi tóxica às lagartas e, portanto, não foi possível determinar os seus parâmetros de toxicidade CL 50 e CL 90 . As proteínas Cry1Aa, Cry1Ab e Cry1Ac são capazes de se ligar a um mesmo receptor nas membranas intestinais, o que aumenta o risco do desenvolvimento de resistência cruzada. Portanto, a utilização conjunta dessas proteínas deve ser evitada.Termos para indexação: Bacillus thuringiensis, manejo da resistência, manejo integrado de pragas, piramidação de genes, proteínas inseticidas, vesículas de membrana. Toxicity and binding capacity of Cry1 proteins to Helicoverpa armigera (Lepidoptera: Noctuidae) intestine receptorsAbstract -The objective of this work was to evaluate the toxicity and the binding capacity of the Cry1Aa, Cry1Ab, Cry1Ac, and Cry1Ca proteins, from Bacillus thuringiensis, to Helicoverpa armigera intestine receptors. Binding analysis of the activated proteins to the brush-border membrane vesicles (BBMV) in the midgut of H. armigera, besides heterologous competition assays to evaluate their binding capacity, was performed. Cry1Ac stood out as the most toxic protein, followed by Cry1Ab and Cry1Aa. The Cry1Ca protein had no toxicity to the caterpillars and, therefore, it was not possible to evaluate its LC 50 and LC 90 toxicity parameters. The Cry1Aa, Cry1Ab, and Cry1Ac proteins are able to bind themselves to the same receptor in the midgut membrane, which increases the risk of developing cross-resistance. Therefore, the use of these proteins together should be avoided.

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“…Our results suggest that in ACB-AhR resistant population a step shared between Cry1Ah and Cry1Fa toxins was affected, while in the ACB-FR resistant population a step involved in Cry1Fa action but not in Cry1Ah was affected. The presence of multiple binding sites shared by different Cry toxins have been documented before, for example the binding competition assays previously performed in Diatraea saccharalis that showed that Cry1Ac and Cry1Ab share binding sites, while Cry1Fa shared binding sites with Cry1Ab but not with Cry1Ac toxin 42 . These results indicate that in D .…”

Section: Discussionmentioning

confidence: 68%

“…These results indicate that in D . saccharalis Cry1Ab toxin may have two binding sites, one that is shared with Cry1Ac and the other shared with Cry1Fa 42 . Our data suggest that in the two O .…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Cry1Ah resistance in Asian corn Borer and its cross-resistance to other Bacillus thuringiensis toxins

Shabbir

Quan

Wang

et al. 2018

Sci Rep

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Transgenic crops producing insecticidal proteins are effective to manage lepidopteran pests. Development of insect-resistance is the major threat to Bacillus thuringiensis (Bt) crops such as Cry1Ah-Maize. Laboratory selection with Bt-Cry1Ah toxin incorporated in artificial diet, during 48 generations of Asian corn borer (ACB) Ostrinia furnacalis produced 200-fold resistance. This resistant colony ACB-AhR readily consumed and survived on Cry1Ah-expressing Bt-maize. Cross-resistance analysis showed high cross-resistance to Cry1F (464-fold), moderate cross-resistance to Cry1Ab (28.38-fold), Cry1Ac (22.11-fold) and no cross-resistance to Cry1Ie toxin. This ACB-AhR cross-resistant phenotype is different from ACB-Cry1Fa resistant population that showed no cross resistance to Cry1Ah, suggesting that different mechanisms of resistance were selected in these two populations. Bioassays of reciprocal F1 crosses-progeny suggested autosomal inheritance of Cry1Ah resistance with no maternal effects. The dominance of resistance increased as concentration decreased. In Cry1Ah-maize tissues the progeny of reciprocal F1 crosses behaved as functionally recessive. Progenies analysis from backcrosses (F1 × resistant strain) suggested polygenic contribution to Cry1Ah- resistance in ACB-AhR. The use of multiple toxins is an imperative factor for delaying evolution of resistance to Cry1Ah-corn in ACB. However, the fact that ACB-AhR showed cross resistance to Cry1Fa indicates that selection of toxins for pyramided plants should be carefully done.

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Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)

Cited by 9 publications

References 25 publications

Diversity of plant growth-promoting bacteria associated with sugarcane

Diversity of plant growth-promoting bacteria associated with sugarcane

Toxicidade e capacidade de ligação de proteínas Cry1 a receptores intestinais de Helicoverpa armigera (Lepidoptera: Noctuidae)

Characterization of the Cry1Ah resistance in Asian corn Borer and its cross-resistance to other Bacillus thuringiensis toxins

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