Binding analysis for interaction of diacetylcurcumin with β-casein nanoparticles by using fluorescence spectroscopy and molecular docking calculations

Mehranfar, Fahimeh; Bordbar, Abdol‐Khalegh; Fani, Najmeh; Keyhanfar, Mehrnaz

doi:10.1016/j.saa.2013.06.062

Cited by 23 publications

(12 citation statements)

References 32 publications

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“…124 Enhanced cytotoxic effect in MCF-7 cells and increased solubility, bioavailability, and anti-tumour activities were observed in a complex composed of a curcumin derivative, Diacetylcurcumin (DAC), and a micellular nanoparticle β-casein. 125 Francis et al 126 created an optimized protocol to produce bis-demethoxy curcumin analogue nanoparticles (BDMCA-NP) that exhibit good anti-cancer therapeutic activities against MCF-7 cells. BDMCA-NPs effectively demonstrated anti-cancer activity by increased apoptosis, G2/M cell cycle arrest, cell death induction through the mitochondrial pathway, and the disassembly of the mitotic spindle of breast cancer cells.…”

Section: The Effect Of Curcumin On Various Breast Cancer Signaling Pamentioning

confidence: 99%

Recent advances of curcumin and its analogues in breast cancer prevention and treatment

2015

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More than 230,000 diagnosed cases of invasive breast cancer in women was estimated in 2014 and an expected 40,000 deaths attributed to the aggressive carcinoma. An effective approach to diminish the morbidity and mortality of breast cancer is the development of chemopreventive and chemotherapeutic agents. Nutraceuticals have demonstrated their ability to proficiently halt carcinogenesis. The administration of natural compounds able to effectively serve as chemoprevention and chemotherapeutics without causing harm or adverse effects is imperative. Curcumin derived from the rhizome of Curcuma longa L., is a common spice of India, used for centuries because of its medicinal properties. The main component of curcumin possesses a wide range of biological activities; anti-proliferative, anti-inflammatory, and apoptotic characteristics modulated through the inactivation of pathways such as EGK and Akt/mTOR. In addition, curcumin alters the expression of cytokines, transcription factors, and enzymes involved in cell vitality. The in vivo application of curcumin in breast cancer is hindered by its limited bioavailabiity. The synthesis of curcumin analogues and delivery via nanoparticles has demonstrated enhanced bioavailability of curcumin in the malignancy. This review focuses on recent developments in the use of curcumin, curcumin analogues, and novel delivery systems as a preventive and therapeutic method for breast cancer.

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Section: The Effect Of Curcumin On Various Breast Cancer Signaling Pamentioning

confidence: 99%

Recent advances of curcumin and its analogues in breast cancer prevention and treatment

2015

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“…Docking experiments were carried out to visualize the binding site of BIM to α-casein. All the docking calculations were performed using Autodock 4.2 Tools [33,34] . The macromolecule was kept rigid, while all the torsional bonds of ligands were set free to rotate.…”

Section: Molecular Dockingmentioning

confidence: 99%

“…Eqs. (7)-(9) made it possible to calculate that R o ¼ 3:30 nm, r ¼ 2:78 nm, J ¼ 8:11 Â 10 À15 cm 3 mol À1 L. The distance between the donor and acceptor ðrÞ is less than 7 nm, indicating that the mechanism of energy transfer for quenching is non-radiative [33,34] . The short distance values suggested a strong interaction between BIM and tryptophan residues in β-casein.…”

Section: Energy Transfermentioning

confidence: 99%

A combination of spectroscopic and molecular docking techniques to study interaction of bis(indolyl)methane with bovine milk α-casein

Dezhampanah

Esmaili

Khorshidi

2017

International Journal of Food Properties

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“…The amphiphilic nature of β‐casein at neutral pH values causes it to assemble into micelles through self‐association. A single β‐casein molecule has a radius of gyration of 4.6 nm and an isoelectric pH of 5.33 (Mehranfar et al ., ), while micelles consisting of 15–60 β‐casein molecules have Rg values of 7.3–13.5 nm (Mehranfar et al ., ). It has been reported that due to its ‘natively unfolded’ structure, casein protein is more stable to heating than whey protein and that the extent of dissociation or aggregation of casein protein from micelles depends on the temperature and duration of heat treatment (Qi et al ., ; Rahimi Yazdi & Corredig, ; Pinto et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Interaction of β‐casein with (−)‐epigallocatechin‐3‐gallate assayed by fluorescence quenching: effect of thermal processing temperature

Chen

Moser

et al. 2015

Int J of Food Sci Tech

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The interactions between the flavan-3-ol (À)-epigallocatechin-3-gallate (EGCG) and bovine b-casein in phosphate-buffered saline (PBS) of pH 6.5 subjected to thermal processing at various temperatures (25-100°C) were investigated using fluorescence quenching. The results indicated that different temperatures had different effects on the structural changes and EGCG-binding ability of b-casein. At temperatures below 60°C, the b-casein-EGCG interaction changed little (P > 0.05) with increasing temperature. At temperatures above 80°C, native assemblies of b-casein in solution dissociated into individual b-casein molecules and unfolded, as demonstrated by a red shift of the maximum fluorescence emission wavelength (k max ) of up to 8.8 nm. The highest quenching constant (K q ) and the number of binding sites (n) were 0.92 (AE0.01) 9 10 13 M À1 s À1 and 0.73 (AE0.02) (100°C), respectively. These results provide insight into the potential of interactions between b-casein-EGCG that may modulate bioactivity or bioavailability to be altered during thermal process.

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Binding analysis for interaction of diacetylcurcumin with β-casein nanoparticles by using fluorescence spectroscopy and molecular docking calculations

Cited by 23 publications

References 32 publications

Recent advances of curcumin and its analogues in breast cancer prevention and treatment

Recent advances of curcumin and its analogues in breast cancer prevention and treatment

A combination of spectroscopic and molecular docking techniques to study interaction of bis(indolyl)methane with bovine milk α-casein

Interaction of β‐casein with (−)‐epigallocatechin‐3‐gallate assayed by fluorescence quenching: effect of thermal processing temperature

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