Bimolecular Fluorescent Complementation (BiFC) by MAP Kinases and MAPK Phosphatases

Schweighofer, Alois; Shubchynskyy, Volodymyr; Kazanavičiūtė, Vaiva; Djamei, Armin; Meskiene, Irute

doi:10.1007/978-1-4939-0922-3_12

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…These observations are also consistent with a recent comprehensive analysis of the Arabidopsis proteome, which covers more than 14 000 proteins and where in ambient conditions the overall MKP1 abundance exceeds by far that of AP2C1 ( http://athena.proteomics.wzw.tum.de/ ) ( Mergner et al , 2020 ), underlining the rather specific role of AP2C1 under stress conditions. The AP2C1 paralogues AP2C2 and AP2C3 ( Umbrasaite et al , 2010 ; Umbrasaite et al , 2011 ; Schweighofer et al , 2014 ) as well as MKP1 and PTP1 interact with the same MAPKs and dephosphorylate them to various extents ( Bartels et al , 2009 ). However, the rather mild phenotypes of ap2c2 mkp1 and ap2c3 mkp1 plants, and the WT-like appearance of ap2c1 ptp1 ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Dual control of MAPK activities by AP2C1 and MKP1 MAPK phosphatases regulates defence responses in Arabidopsis

Ayatollahi

Kazanavičiūtė

Shubchynskyy

et al. 2022

Journal of Experimental Botany

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Mitogen-activated protein kinase (MAPK) cascades transmit environmental signals and induce stress and defence responses in plants. These signalling cascades are negatively controlled by specific phosphatases of the type 2C Ser/Thr protein phosphatase (PP2C) and dual-specificity phosphatase (DSP) families that inactivate stress-induced MAPKs; however, the interplay between phosphatases of these different types has remained unknown. Our work reveals that different Arabidopsis MAPK phosphatases, the PP2C-type AP2C1 and the DSP-type MKP1, exhibit both specific and overlapping functions in plant stress responses. Each single mutant and ap2c1 mkp1 double mutant displayed enhanced stress-induced activation of MAPKs MPK3, MPK4, and MPK6, as well as induction of a set of transcription factors. Moreover, ap2c1 mkp1 double mutants show an autoimmune-like response, associated with elevated levels of the stress hormones salicylic acid and ethylene, and of the phytoalexin camalexin. Interestingly, this phenotype is reduced in ap2c1 mkp1 mpk3 and ap2c1 mkp1 mpk6 triple mutants, suggesting that the autoimmune-like response is due to MAPK misregulation. We conclude that the evolutionarily distant MAPK phosphatases AP2C1 and MKP1 contribute crucially to the tight control of MAPK activities, ensuring appropriately balanced stress signalling and suppression of autoimmune-like responses during plant growth and development.

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Section: Discussionmentioning

confidence: 99%

Dual control of MAPK activities by AP2C1 and MKP1 MAPK phosphatases regulates defence responses in Arabidopsis

Ayatollahi

Kazanavičiūtė

Shubchynskyy

et al. 2022

Journal of Experimental Botany

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“…Arabidopsis thaliana cell suspension cultures (ACSC) are an amenable, simplified model in which to study specific signaling mechanisms that would be too complex in plant tissues or organs. For instance, ACSC were recently used to study sugar signaling (Kunz et al, 2014), MAPK kinase and phosphatase signaling (Schweighofer et al, 2014), chitosan and galacturonide elicitor signaling (Ledoux et al, 2014), photooxidative damage (Gutiérrez et al, 2014), auxin transmembrane transport (Seifertová et al, 2014) and ion channel activity (Haapalainen et al, 2012). ACSC are easily labeled which is convenient for studying metabolic fluxes (Tjellström et al, 2012) like those in lipid phospholipid signaling.…”

Section: Introductionmentioning

confidence: 99%

Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome

et al. 2014

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Basal phosphoinositide-dependent phospholipase C (PI-PLC) activity controls gene expression in Arabidopsis suspension cells and seedlings. PI-PLC catalyzes the production of phosphorylated inositol and diacylglycerol (DAG) from phosphoinositides. It is not known how PI-PLC regulates the transcriptome although the action of DAG-kinase (DGK) on DAG immediately downstream from PI-PLC is responsible for some of the regulation. We previously established a list of genes whose expression is affected in the presence of PI-PLC inhibitors. Here this list of genes was used as a signature in similarity searches of curated plant hormone response transcriptome data. The strongest correlations obtained with the inhibited PI-PLC signature were with salicylic acid (SA) treatments. We confirm here that in Arabidopsis suspension cells SA treatment leads to an increase in phosphoinositides, then demonstrate that SA leads to a significant 20% decrease in phosphatidic acid, indicative of a decrease in PI-PLC products. Previous sets of microarray data were re-assessed. The SA response of one set of genes was dependent on phosphoinositides. Alterations in the levels of a second set of genes, mostly SA-repressed genes, could be related to decreases in PI-PLC products that occur in response to SA action. Together, the two groups of genes comprise at least 40% of all SA-responsive genes. Overall these two groups of genes are distinct in the functional categories of the proteins they encode, their promoter cis-elements and their regulation by DGK or phospholipase D. SA-regulated genes dependent on phosphoinositides are typical SA response genes while those with an SA response that is possibly dependent on PI-PLC products are less SA-specific. We propose a model in which SA inhibits PI-PLC activity and alters levels of PI-PLC products and substrates, thereby regulating gene expression divergently.

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“…These observations are also consistent with a recent comprehensive analysis of the Arabidopsis proteome, which covers more than 14,000 proteins and where in ambient conditions the overall MKP1 abundance outnumbers by far that of AP2C1 (http://athena.proteomics.wzw.tum.de/) (Mergner et al ., 2020), underlining the rather specific role of AP2C1 under stress conditions. The AP2C1 paralogues AP2C2 and AP2C3 (Schweighofer et al ., 2014; Umbrasaite et al ., 2010; Umbrasaite et al ., 2011) as well as MKP1 and PTP1 interact with the same MAPKs and dephosphorylate them to various extents (Bartels et al ., 2009). However, the rather mild phenotypes of ap2c2 mkp1 and ap2c3 mkp1 plants and the WT-like appearance of ap2c1 ptp1 (this work) compared to ap2c1 mkp1 plants clearly indicate specific genetic interactions and redundant functions of the evolutionary distant AP2C1 and MKP1 phosphatases in the regulation of signalling pathways.…”

Section: Discussionmentioning

confidence: 99%

Dual control of MAPK activities by AP2C1 and MKP1 MAPK phosphatases regulates defence responses in Arabidopsis

Ayatollahi

Kazanaviciute

Shubchynskyy

et al. 2021

Preprint

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Mitogen-activated protein kinase (MAPK) cascades transmit environmental signals and induce stress and defence responses in plants. These signalling cascades are negatively controlled by specific phosphatases of the type 2C Ser/Thr protein phosphatase (PP2C) and dual-specificity phosphatase (DSP) families that inactivate stress-induced MAPKs; however, the interplay between phosphatases of these different types has remained unknown. Our work reveals that different Arabidopsis MAPK phosphatases, the PP2C-type AP2C1 and the DSP-type MKP1, exhibit both specific and overlapping functions in plant stress responses. Each single mutant and ap2c1 mkp1 double mutant displayed enhanced wound-induced activation of MAPKs MPK3, MPK4, and MPK6, as well as induction of a set of transcription factors. Moreover, ap2c1 mkp1 double mutants show an autoimmune-like response, associated with elevated levels the stress hormones salicylic acid and ethylene, and of the phytoalexin camalexin. Interestingly, this phenotype is reduced in ap2c1 mkp1 mpk6 triple mutants, suggesting that the autoimmune-like response is due to MPK6 misregulation. We conclude that the evolutionarily distant MAPK phosphatases AP2C1 and MKP1 contribute crucially to the tight control of MPK6 activity, ensuring appropriately balanced stress signalling and suppression of autoimmune-like responses during plant growth and development.

show abstract

Bimolecular Fluorescent Complementation (BiFC) by MAP Kinases and MAPK Phosphatases

Cited by 6 publications

References 38 publications

Dual control of MAPK activities by AP2C1 and MKP1 MAPK phosphatases regulates defence responses in Arabidopsis

Dual control of MAPK activities by AP2C1 and MKP1 MAPK phosphatases regulates defence responses in Arabidopsis

Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome

Dual control of MAPK activities by AP2C1 and MKP1 MAPK phosphatases regulates defence responses in Arabidopsis

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