Bimolecular chemistry of macromolecules: synthesis of bacterial polysaccharide conjugates with Neisseria meningitidis membrane protein

Immunogenicity of Haemophilus Influenzae Type b Conjugate Vaccines in Infant Rhesus Monkeys

“…Hib polysaccharide-meningococcal OMPC conjugate vaccine (PedvaxHIB, Merck Sharp and Dohme, West Point, PA) contains PRP covalently coupled to an OMPC from Neisseria meningitidis serogroup B by a thioether linkage (10). Hib oligosaccharide-CRM19, conjugate vaccine (HibTITER, Praxis Biologics, Rochester, NY) contains Hib oligosaccharides coupled by reductive amination to a carrier protein (CRM197) that is a nontoxic mutant of diphtheria toxin (1 1).…”

A major crossreactive idiotype associated with human antibodies to the Haemophilus influenzae b polysaccharide. Expression in relation to age and immunoglobulin G subclass.

“…Sera from 1 13 healthy children and adults who were immunized with one dose of Hib PS vaccine (n = 98), or a conjugate of Hib PS coupled to a Neisseria meningitidis group B outer membrane protein complex (Hib PS-OMP [ 15]) (n = 15) were available for this study. The serum samples were obtained immediately before immunization and 1-2 mo later and were stored at -70'C.…”

IgG Subclass Response to Immunization with Haemophilus influenzas Type b Polysaccharide-Outer Membrane Protein Conjugate Vaccine1

“…A total of 117 healthy children was vaccinated with conventional Hib PS vaccine or a conjugate of Hib PS coupled to a partially purified N. meningitidis OMP (24). Informed consent was obtained from one or both parents of the children.…”