Bile acids serum levels in patients with nonalcoholic fatty liver disease

Abstract

Aim. To estimate the blood levels of primary, secondary, tertiary and unconjugated bile acids in patients with non-alcoholic fatty liver disease.Methods. The study included 74 patients with non-alcoholic fatty liver disease (males - 30, females - 44) and 51 healthy individuals (males - 14, females - 37). All patients underwent anthropometry and complete clinical, biochemical and instrumental examination (measuring the subcutaneous fat layer). 64 patients had hepatic steatosis, 10 - steatohepatitis. Serum levels of bile acids (primary: cholic, chenodeoxycholic; secondary: lithocholic, deoxycholic and tertiary: ursodeoxycholic) were measured by gas-liquid chromatography on «Chromos GC-1000» (Russia) scanner.Results. Unconjugated primary, secondary and tertiary bile acids were detected in the blood of healthy individuals and patients with non-alcoholic fatty liver disease. In healthy individuals, there were no gender differences found in the bile acids levels. Patients with non-alcoholic fatty liver disease had higher level of bile acids compared to healthy controls. There was a significant difference in the concentrations of secondary and tertiary bile acids in patients with hepatic steatosis and steatohepatitis.Conclusion. Blood bile acids levels were significantly higher in patients with non-alcoholic fatty liver disease than in healthy individuals. At steatohepatitis, females had higher levels of cholic, chenodeoxycholic and deoxycholic acids and lower levels of lithocholic and ursodeoxycholic acids compared to males. Significant difference in patients with hepatic steatosis and steatohepatitis was revealed only in levels of secondary and tertiary bile acids.

The role of serum bile acid profile in differentiation between nonalcoholic fatty liver disease and chronic viral hepatitis

“…We found that only 4 bile acids, 2 primary (CA, CDCA), 1 glycine conjugated (GCDCA), and 1 secondary (DCA), had statistically significant increase in the mean serum levels in NAFLD group compared to [17,18].…”

Evaluation of enterohepatic circulation of the bile acids in patients with non-alcoholic fatty liver disease

“…Неалкогольная жировая болезнь печени (НАЖБП) представляет собой полиэтиологическое заболевание, склонное к прогрессированию [1][2][3][4]. В патогенезе поражения печени определенное значение придается нарушению энтерогепатической циркуляции желчных кислот (ЖК) [5][6][7][8][9][10]. В печени синтезируются первичные ЖК -холевая и хенодезоксихолевая, которые конъюгируют с аминокислотами глицином и таурином и поступают в виде конъюгатов в двенадцатиперстную кишку, где участвуют в эмульгировании экзогенных жиров, всасывании холестерина и жирорастворимых витаминов.…”