Bile Acids

Jt, Matschiner; Ta, Mahowald; Wh, Elliott; Ea, Doisy; Sl, Hsia

doi:10.1016/s0021-9258(18)64876-8

Cited by 105 publications

(2 citation statements)

References 12 publications

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“…Chromatography and Other Methods. Bile acids and sterols were purified by acetic acid partition chromatography (Matschiner et al, 1957). In this system the fractions have been designated according to the percentage of benzene in hexane; e.g., fraction 60-2 represents the second fraction of the eluent containing 60% benzene in hexane.…”

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confidence: 99%

Comparison of 12α-hydroxylation of oxygenated 5α-cholestanes and allochenodeoxycholate with rat liver microsomes

Mui¹,

Elliott²

1975

Biochemistry

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[5alpha,6alpha-3H2]-5alpha-Cholestane-3alpha,7alpha-diol (A), (25 R)-3alpha,7alpha-dihydroxyl-[5alpha,6alpha-3H2]-5alpha--cholestan-26-oic acid (B),(25R)-[5alpha,6alpha-3H2]stane-3alpha,7alpha-26-triol (C), and [3beta-3H]allochenodeoxycholic acid (D) were prepared, characterized, and studied with a rat liver microsomal preparation fortified with 1 mM NADPH. The 12alpha-hydroxylated product formed from each of these substrates was identified by isotopic dilution; the relative reactivity of the four substrates was (A) 100; (B) 87; (C) 135; and (D) 40. The microsomal system showed a requirement for added NADPH and other properties similar to those shown for 12alpha-hydroxylation of 7alpha-hydroxycholest-4-en-3-one, which is ultimately converted to cholic acid. Since the coplanar 5alpha-cholestane-3alpha,7alpha-diol is virtually superimposable upon 7alpha-hydroxycholest-4-en-3-one, and the enzymic requirements are comparable, it is suggested that a single enzyme system may be responsible for 12alpha-hydroxylation of these substrates.

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confidence: 99%

Comparison of 12α-hydroxylation of oxygenated 5α-cholestanes and allochenodeoxycholate with rat liver microsomes

Mui¹,

Elliott²

1975

Biochemistry

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“…Chromatography. Bile acids were chromatographed on an acetic acid partition column as previously described (Matschiner et al, 1957). The fractions have been designated according to the percentage of benzene in hexane.…”

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confidence: 99%