Bile acid profiles in bile and feces of obese mice by a high‐performance liquid chromatography–tandem mass spectrometry

Abstract

Bile acids (BAs) play a pivotal role in manipulating the development of metabolic diseases. However, due to the compositional complexity and functional variation of BAs, it remains unclear about the changes in BA pool for individuals with obesity or metabolic syndrome. We established a high-performance liquid chromatography-mass spectrometer detection system for the simultaneous analysis of both unconjugated and conjugated BAs in the bile and feces of mice. Ten BAs were completely separated, identified, and quantified with low limit of detection (0.5 ng/mL) and inter/intraday precision (relative standard deviation < 12%). By using this method, these BAs in bile and feces of mice were quantified. The result showed that taurochenodeoxycholic acid, taurine-conjugated α-muricholic acids, and taurine-conjugated β-muricholic acids were the dominated BAs in bile, whereas deoxycholic acid and chenodeoxycholic acid predominated in feces. Further, most of the BA levels were significantly elevated in either bile or fecal samples of high-fat diet-fed mice as compared with those in normal chow diet-fed mice, indicating that excessive production of BAs was closely associated with the occurrence of lipid metabolism disorders. In summary, the present method is practicable for analysis of BAs in bile and fecal samples of patients with obesity.

Fucoidan Ameliorated Dextran Sulfate Sodium-Induced Ulcerative Colitis by Modulating Gut Microbiota and Bile Acid Metabolism

“…Finally, the sample was resolved with 100 μL of methanol/water (v/v = 17:3) and filtered by a 0.22 μm membrane for HPLC/MS analysis. The HPLC/MS system (Agilent 1200, USA) equipped with an Acuity UPLC BEH C18 column (internal diameter, 2.1 × 100 mm; 1.7 μm; Waters, USA) was applied for separating 20 bile acids as previously described. , The obtained data were conducted for principal component analysis (PCA). The level of BAs in the cecum and Pearson’s rank correlation coefficient with gut microbiota were statistically calculated with RStudio software.…”

Bletilla striata Oligosaccharides Improve Ulcerative Colitis by Regulating Gut Microbiota and Intestinal Metabolites in Dextran Sulfate Sodium-Induced Mice

“…After filtration with 0.22 μm microporous membranes, all samples were analyzed by a liquid chromatography-mass spectrometer (LC-MS). The LC-MS parameters referred to previous studies ( Zheng et al, 2021 ). For the analysis of SCFAs and tryptophan metabolites, 50 mg of stool sample was dissolved in 1 ml 50% (V/V) methanol aqueous solution (containing 0.2% HCl).…”

The effects of elemene emulsion injection on rat fecal microbiota and metabolites: Evidence from metagenomic exploration and liquid chromatography-mass spectrometry

“…Third, let the sample stand at −20°C for 30 min, centrifuge for 15 min (13,000 g , 4°C), and then transfer the supernatant to the inlet with an inner cannula. In addition, 20 μL of supernatant was pipetted from each sample and mixed as a quality control sample (Zheng et al, 2021 ; Zhu et al, 2022 ).…”