Bile acid derivatives from a sponge-associated bacterium Psychrobacter sp.

Li, Huayue; Shinde, Pramod B.; Lee, Hye Ja; Yoo, Eun Sook; Lee, Chong‐O.; Hong, Jongki; Choi, Sang Ho; Jung, Jee H.

doi:10.1007/s12272-009-1607-1

Cited by 31 publications

(27 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Members of this genus have previously been described to harbour bioactive compounds and dehalogenases2931. The halogenase gene from this isolate has a very high sequence homology to one of the cDNA sequences obtained from the microbial community of C. crambe , indicating that the gene could be detected by culture dependent and independent methods and is expressed within the microbial community, although Psychrobacter sp.…”

Section: Discussionmentioning

confidence: 87%

See 1 more Smart Citation

Culture-dependent and independent approaches for identifying novel halogenases encoded by Crambe crambe (marine sponge) microbiota

Öztürk

Jaeger

Smidt

et al. 2013

Sci Rep

View full text Add to dashboard Cite

Sponges harbour microbial communities that contribute to the genetic and metabolic potential of their host. Among metabolites produced by sponge-associated microbial communities, halogenated compounds are of special interest because of their biotechnological potential. In this study, we have examined the diversity of the cultivable fraction of the marine demosponge Crambe crambe microbiota. Application of complementary cultivation methods yielded 107 bacterial isolates, some of which may be sponge-specific based on their phylogenetic analysis. Among these, Psychrobacter sp. was found to contain a putative halogenase gene. In addition to the culture-dependent approach for discovering halogenase genes, a cDNA library was constructed to determine the diversity of halogenase genes expressed in situ by the C. crambe microbiota. To this end, seventeen putative tryptophan halogenase cDNA sequences were identified, most of which were only remotely related to known halogenase genes, indicating the potential for novel bioactive compounds being produced by the C. crambe microbiota.

show abstract

Section: Discussionmentioning

confidence: 87%

“…In contrast, using the same primers, only one of the 107 isolates (isolate 23D8, Psychrobacter sp) was found to possess a putative halogenase gene. By comparison to the NCBI database of microbial proteins and the Conserved Domains Database29, all of these the retrieved sequences were classified as putative tryptophan halogenases.…”

Section: Resultsmentioning

confidence: 99%

Culture-dependent and independent approaches for identifying novel halogenases encoded by Crambe crambe (marine sponge) microbiota

Öztürk

Jaeger

Smidt

et al. 2013

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The purity of all products was determined by TLC, the results showed one pot in TLC plates using several different polar solvent. The targeting compounds were characterized by 1 H, 13 C NMR and LC-MS spectrometry.…”

Section: Resultsmentioning

confidence: 99%

“…IR spectra were acquired using a Bruker Tensor 27 spectrometer. The 1 H-NMR (600 MHz, CDCl 3 containing TMS) and 13 C-NMR (150 MHz, CDCl 3 containing TMS) spectra were obtained using a Bruker AV-600 spectrometer. The J values are given in hertz.…”

Section: Methodsmentioning

confidence: 99%

“…A literature survey of antimicrobial steroids revealed that several amino steroid derivatives exhibit profound antimicrobial activity. The preparation of various bile acid-based amino sterols was reported with a view to examine their activity as antimicrobial agents [10][11][12][13]. A recent approach to combat pathogens is to introduce a cationic chain onto a steroid scaffold that plays key role in biological systems.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cholic Acid‐Derived Facial Surfactants with Long Side‐Chain Quaternary Ammonium: Synthesis and Antimicrobial Activity Study

Zhou

et al. 2016

J Surfact & Detergents

View full text Add to dashboard Cite

Four cholic acid cationic surfactants containing a flexible long aliphatic chain were synthesized efficiently. A long aliphatic chain was introduced first on the side‐chain carboxyl of cholic acid by one‐pot condensation reaction of cholic acid and C12–C18 amines using DCC (Dicyclohexylcarbodiimide) and HOBt (N‐hydroxybenzotrizole) as condensation agents for the formation of an amide framework. Then reduction of the amide and quaternization gave a cationic group for strengthening hydrophilicity. This strategy offered a very straightforward and efficient method for access to the designed surfactants in good overall yields. Preliminary results show that an increase both in the length of the hydrophobic tail and in the number of charged groups lowered the CMC of cholic acid‐derived cationic surfactants. Cholic acid‐based cationic surfactants containing a flexible longer aliphatic chain and a quaternary ammonium had the highest antimicrobial activity.

show abstract

Studies on the Secondary Metabolites of a Pseudoalteromonas sp. Isolated from Sediments Collected at the Northeastern Coast of Brazil

Arthaud

Rodrigues

Jimenez

et al. 2012

Chemistry & Biodiversity

View full text Add to dashboard Cite

Continuing search for anticancer compounds from the marine environment, we have studied microorganisms that inhabit intertidal sediments of the northeastern Brazilian coast. Of the 32 strains isolated, 13 were selected for biological evaluation of their crude extracts. The acetate extract obtained from a Gram-negative bacterium was strongly active against cancer cell lines with IC(50) values that ranged from 0.04 (HL60 leukemia cells) to 0.26 μg/ml (MDA MB-435 melanoma cells). The bacterium was identified as a Pseudoalteromonas sp. based on 16S rRNA gene sequencing. A bioassay-guided fractionation of the active extract led to the isolation of prodigiosin, a well-known tripyrrole red pigment with immunosuppressive and anticancer activities. Further experiments with ErbB-2 overexpressing cell lines, including HB4a-C3.6 (moderate overexpression), HB4a-C5.2 (high overexpression), and the parental HB4a cell line, were performed. Prodigiosin was moderately active toward HB4a cells with an IC(50) of 4.6 μg/ml, while it was 115 and 18 times more active toward HB4a-C3.6 cells (IC(50) of 0.04 μg/ml) and HB4a-C5.2 (IC(50) of 0.26 μg/ml) cells, respectively. These data suggest that, in spite of its previously described apoptosis-inducing properties, prodigiosin can selectively recognize cells overexpressing ErbB-2, which could be highly appealing in human breast cancer therapy.

show abstract

Bile acid derivatives from a sponge-associated bacterium Psychrobacter sp.

Cited by 31 publications

References 16 publications

Culture-dependent and independent approaches for identifying novel halogenases encoded by Crambe crambe (marine sponge) microbiota

Culture-dependent and independent approaches for identifying novel halogenases encoded by Crambe crambe (marine sponge) microbiota

Cholic Acid‐Derived Facial Surfactants with Long Side‐Chain Quaternary Ammonium: Synthesis and Antimicrobial Activity Study

Studies on the Secondary Metabolites of a Pseudoalteromonas sp. Isolated from Sediments Collected at the Northeastern Coast of Brazil

Contact Info

Product

Resources

About