Bilayered Fibrin-Based Electrospun-Sprayed Scaffold Loaded with Platelet Lysate Enhances Wound Healing in a Diabetic Mouse Model

Losi, Paola; Kayal, Tamer Al; Buscemi, Marianna; Foffa, Ilenia; Cavallo, Alessia; Soldani, Giorgio

doi:10.3390/nano10112128

Cited by 21 publications

(23 citation statements)

References 28 publications

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“…In previous experiments Mepore polyurethane film, used in this study was used as a secondary dressing, was used as control since it does not possess any bioactive properties but it allows to maintain hydration and sterility. In Mepore mice assumed as untreated groups only a 26% of wound closure was observed at days 14 [13]. Moreover, the semiquantitative histopathological analysis showed that Plg-loaded fibrin scaffold induce a significantly higher re-epithelialization and collagen deposition and a lower inflammation respect to Plg-unloaded fibrin scaffold.…”

Section: Discussionmentioning

confidence: 91%

“…Recently our group developed a process that does not required any further treatment, such as washing steps, to produce a bilayered fibrin/polyurethane scaffold loaded with platelet lysate by a combination of electrospinning and spray, phase-inversion method for wound healing. The scaffold is composed of a nanostructured fibrin layer with bioactive function obtained by electrospinning and a polyurethane layer obtained by spray technology to confer mechanical support [13].…”

Section: Discussionmentioning

confidence: 99%

“…The healing area was measured according to the previously described protocol [13]. At 0, 7 and 14 days, wounds were photographed, and the wound area was measured by AxioVision Rel.…”

Section: Calculation Of Wound Areamentioning

confidence: 99%

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Plasminogen-Loaded Fibrin Scaffold as Drug Delivery System for Wound Healing Applications

et al. 2022

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Plasminogen is a protein involved in intravascular and extravascular fibrinolysis, as well as in wound healing, cell migration, tissue formation and angiogenesis. In recent years its role in healing of tympanic perforations has been demonstrated in plasminogen deficient mice. The aim of this work was to fabricate a fibrin-based drug delivery system able to provide a local and sustained release of plasminogen at the wound site. Initially, the biological activity of plasminogen was evaluated by in vitro experiments on cell cultures. A metabolic assay (MTT) was carried out on L929 mouse fibroblast to determine the concentration that does not affect cell viability, which turned out to be 64 nM. The effect of plasminogen on cell migration was evaluated through a scratch test on human keratinocytes: cells treated with 64 nM plasminogen showed faster scratch closure than in complete medium. Fibrin scaffold loaded with plasminogen was fabricated by a spray process. SEM analysis showed the typical nano-fibrillar structure of a fibrin scaffold. Tensile tests highlighted significantly higher value of the ultimate stress and strain of fibrin scaffold with respect to fibrin clot. The in-vitro release kinetic showed an initial plasminogen burst, after that the release slowed, reaching a plateau at 7 days. Plasminogen-loaded fibrin scaffold applied in full-thickness diabetic mouse lesions showed a significantly higher closure rate at 14 days than scaffold used as a reference material. Histological analysis demonstrated an improved reepithelization and collagen deposition in granulation tissue in mouse treated with plasminogen-loaded fibrin scaffold in comparison to unloaded fibrin scaffold. The obtained results demonstrated the suitability of the fibrin scaffold loaded with plasminogen as drug delivery system and suggest its use in wound healing applications, such as for the treatment of chronic diabetic ulcers.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Plasminogen-Loaded Fibrin Scaffold as Drug Delivery System for Wound Healing Applications

et al. 2022

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“…The cumulative release of PDGF and VEGF from FB-PL fiber membranes was detected by the ELISA method. The results showed that 40% growth factor was released from FB-PL fiber membranes on the first d and 80% on the 7th d [84]. CTGF is unstable in a highly oxidized diabetic wound environment, and the use of nanofiber membranes loaded with CTGF can improve its stability and promote diabetic wound healing [99].…”

Section: Drugs/synthetic Polymer Electrospun Fibersmentioning

confidence: 99%

Application of Electrospun Nanofiber Membrane in the Treatment of Diabetic Wounds

et al. 2021

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Diabetic wounds are complications of diabetes which are caused by skin dystrophy because of local ischemia and hypoxia. Diabetes causes wounds in a pathological state of inflammation, resulting in delayed wound healing. The structure of electrospun nanofibers is similar to that of the extracellular matrix (ECM), which is conducive to the attachment, growth, and migration of fibroblasts, thus favoring the formation of new skin tissue at the wound. The composition and size of electrospun nanofiber membranes can be easily adjusted, and the controlled release of loaded drugs can be realized by regulating the fiber structure. The porous structure of the fiber membrane is beneficial to gas exchange and exudate absorption at the wound, and the fiber surface can be easily modified to give it function. Electrospun fibers can be used as wound dressing and have great application potential in the treatment of diabetic wounds. In this study, the applications of polymer electrospun fibers, nanoparticle-loaded electrospun fibers, drug-loaded electrospun fibers, and cell-loaded electrospun fibers, in the treatment of diabetic wounds were reviewed, and provide new ideas for the effective treatment of diabetic wounds.

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“…Physico-mechanical stimulation is another emerging approach to regulate cell behavior and differentiation potential. In this regard, Losi et al reported on the design of a bioactive nanostructured topical medication promoting dermal tissue regeneration, comprising a bilayered fibrin/poly(ether urethane) membrane obtained through a combination of electrospinning and spray phase inversion methods [ 9 ]. Additional loading of platelet lysate into the fibrin counterpart of the device significantly accelerated wound healing in diabetic mice and favored both re-epithelialization and collagen deposition.…”

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confidence: 99%

Biomaterials Tailoring at the Nanoscale for Tissue Engineering and Advanced Therapies

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Ciardelli

2021

Nanomaterials

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Bilayered Fibrin-Based Electrospun-Sprayed Scaffold Loaded with Platelet Lysate Enhances Wound Healing in a Diabetic Mouse Model

Cited by 21 publications

References 28 publications

Plasminogen-Loaded Fibrin Scaffold as Drug Delivery System for Wound Healing Applications

Plasminogen-Loaded Fibrin Scaffold as Drug Delivery System for Wound Healing Applications

Application of Electrospun Nanofiber Membrane in the Treatment of Diabetic Wounds

Biomaterials Tailoring at the Nanoscale for Tissue Engineering and Advanced Therapies

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