Bilayer Stability and Impermeability of Nonionic Surfactant Vesicles Sterically Stabilized by PEG–Cholesterol Conjugates

Beugin-Deroo, Sophie; Ollivon, Michel; Lesieur, Sylviane

doi:10.1006/jcis.1998.5506

Cited by 33 publications

(21 citation statements)

References 31 publications

(40 reference statements)

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“…at the ends of the macromolecule, or poloxamers (7,8). Whether some polymers may modify the structure and stability of the bilayer is much less studied.…”

Section: Introductionmentioning

confidence: 98%

Slow Reorganization of Small Phosphatidylcholine Vesicles upon Adsorption of Amphiphilic Polymers

Ladavière

Toustou

Gulik-Krzywicki

et al. 2001

Journal of Colloid and Interface Science

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“…at the ends of the macromolecule, or poloxamers (7,8). Whether some polymers may modify the structure and stability of the bilayer is much less studied.…”

Section: Introductionmentioning

confidence: 98%

Slow Reorganization of Small Phosphatidylcholine Vesicles upon Adsorption of Amphiphilic Polymers

Ladavière

Toustou

Gulik-Krzywicki

et al. 2001

Journal of Colloid and Interface Science

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“…15,16 It was shown recently 7,[17][18][19][20] that water-soluble polymers, upon binding to vesicles, can markedly affect the shape, curvature, stiffness, or stability of the bilayer. However, the mechanisms of these polymer-induced reorganizations of membranes remain sometimes conjectural, although it is clear that the hydrophobicity of the polymer plays an important role.…”

Section: Introductionmentioning

confidence: 99%

Real-Time Observation of Polyelectrolyte-Induced Binding of Charged Bilayers

Luan

Ramos

2007

J. Am. Chem. Soc.

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Abstract:We present real-time observations by confocal microscopy of the dynamic behavior of multilamellar vesicles (MLVs), composed of charged synthetic lipids, when put in contact with oppositely charged polyelectrolyte (PE) molecules. We find that the MLVs exhibit astonishing morphological transitions, which result from the discrete and progressive binding of the charged bilayers induced by a high PE concentration gradient. Our physical picture is confirmed by quantitative measurements of the fluorescence intensity as the bilayers bind to each other. The shape transitions lead eventually to the spontaneous formation of hollow capsules, whose thick walls are composed of lipid multilayers condensed with PE molecules. This class of objects may have some (bio)technological applications.

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“…The membrane composition can potentially act on vesicle permeability in so far as this can be modulated by PEGylated lipids (45)(46)(47) and lowered by cholesterol (48)(49)(50). The profiles in Fig.…”

Section: Influence Of Bilayer Compositionmentioning

confidence: 98%

Entrapment of a neutral Tm(III)‐based complex with two inner‐sphere coordinated water molecules into PEG‐stabilized vesicles: towards an alternative strategy to develop high‐performance LipoCEST contrast agents for MR imaging

Chahid

Elst

Flament

et al. 2014

Contrast Media & Molecular

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Chemical exchange saturation transfer (CEST) probes issued from the encapsulation of a water proton paramagnetic shift reagent into the inner aqueous volume of lipid vesicles provide an emerging class of frequency-selective contrast agents with huge potential in the field of molecular magnetic resonance imaging (MRI). This work deals with the generation of such LipoCEST agents properly designed to optimize, under isotonic conditions, the chemical shift offset of the intra-liposomal water protons as well as the number of exchangeable protons under reasonably low radiofrequency (RF) fields of saturation. The strategy lies in the loading of poly(ethylene glycol)-stabilized nanosized liposomes with uncharged lanthanide chelates, binding more than one water molecule in the first hydration sphere, exemplified here by [Tm(III)-DO3A (H2 O)2 ] complex. The key properties of the probes are demonstrated by complementary NMR investigations. The residence lifetime of the water molecules coordinated to the lanthanide center was outstandingly short (9.5 ± 0.2 ns from (17) O NMR), and indeed relevant for effective LipoCEST responsiveness. The (1) H NMR CEST spectra (7.01 T magnetic field) prove that the theoretically expected optimal sensitivity can be approximated in the nanomolar concentration range, at reasonably low RF presaturation pulses (6.7-12 μT) and saturation frequency offsets of the intra-liposomal water protons beyond 10 ppm, making possible selective irradiation in biological environment. CEST-MRI images (7.01 T magnetic field and 10-12 μT RF pulse) explicitly confirm the interest of these newly conceived LipoCEST agents, indeed among the most efficient ones developed so far under isosmotic conditions.

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Bilayer Stability and Impermeability of Nonionic Surfactant Vesicles Sterically Stabilized by PEG–Cholesterol Conjugates

Cited by 33 publications

References 31 publications

Slow Reorganization of Small Phosphatidylcholine Vesicles upon Adsorption of Amphiphilic Polymers

Slow Reorganization of Small Phosphatidylcholine Vesicles upon Adsorption of Amphiphilic Polymers

Real-Time Observation of Polyelectrolyte-Induced Binding of Charged Bilayers

Entrapment of a neutral Tm(III)‐based complex with two inner‐sphere coordinated water molecules into PEG‐stabilized vesicles: towards an alternative strategy to develop high‐performance LipoCEST contrast agents for MR imaging

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