Bilayer lipid membranes as electrochemical switches in reactions involving alteration of surface charge

Krull, Ulrich J.; Seethaler, Sherry; Brennan, John D.; Nikolelis, Dimitrios P.

doi:10.1016/0040-6090(94)90600-9

Cited by 13 publications

(11 citation statements)

References 12 publications

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“…Therefore, these values of the apparent Michaelis -Menten constants are in agreement considering the impact of the negative surface potential of the lipid film on the cationic substrate [20,21]. A detailed comparison of the profiles and magnitudes of the current transients obtained in previously published work [16,22,23] and the transients presently observed and previously obtained using flow injection technique for Ach determination [11,24] indicate important similarities and substantial differences. Of greatest significance is the appearance of well-defined transient currents of the same direction in both types of experiment as the hydronium ion concentration at the BLM surface is altered during the enzymatic reactions.…”

supporting

confidence: 85%

“…A slow "channel" formation implies that the majority of ions will be dissipated into the bulk solution; a fast though "channel" opening will result in the passage of an appreciable portion of the ions through the lipid membrane. The former phenomena were observed in previous diffusion controlled alterations of the pH at one side of a BLM (hydrolytic enzyme reactions) with concurrent observations of charging current transients [16,22,23], while the latter phenomena were observed in the flow experiments in which the number of defects dynamically varies as the pH of the carrier electrolyte solution is altered and these results indicate how amplification of analytical signal of BLMbased transducers may be achieved [11,24].…”

Section: à5mentioning

confidence: 59%

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Stabilized Lipid Membrane Based Biosensors with Incorporated Enzyme for Repetitive Uses

et al. 2006

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This work reports a technique for the stabilization of lipid membrane based biosensors with incorporated enzyme that retains its activity for repetitive uses. Microporous filters composed of glass fibers were used as supports for the stabilization of these sensors. The lipid film is formed on the filter by polymerization using UV (ultraviolet) radiation prior its use. Methacrylic acid was the functional monomer, ethylene glycol dimethacrylate was the crosslinker and 2,2'-azobis-(2-methylpropionitrile) was the initiator. The enzyme (acetylcholinesterase) is incorporated within this mixture prior to polymerization. The polymerization process takes place by using UV irradiation instead of heating at 60 8C the lipid mixture because this temperature might denature the enzyme. This method for preparation of stabilized lipid membranes was investigated using Raman spectroscopy. The results have indicated that the kinetics of polymerization are completed within 4 hours. The retain in activity of the enzyme was studied using electrochemical experiments which have shown that this mild technique of polymerization can now be used to incorporate a protein in these lipid membranes without loss of their activity. This will allow the practical use of the techniques for chemical sensing based on lipid membranes based biosensors and commercialization of these devices.

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confidence: 85%

Section: à5mentioning

confidence: 59%

Stabilized Lipid Membrane Based Biosensors with Incorporated Enzyme for Repetitive Uses

et al. 2006

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“…The mechanism of signal generation for freely-suspended or filter supported BLMs has been provided in the literature [ 1 , 2 , 3 ]. In the case of lipid membrane based biosensors that use an enzyme as a transduction/recognition element, the enzyme is located in the lipid film in such a way that the hydrophobic portions of the enzyme is incorporated into the lipid, leaving the active site of the enzyme at the aqueous interface [ 1 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Biosensors Based on Lipid Membranes for the Rapid Detection of Toxins

et al. 2018

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Lipid assemblies in the form of two dimensional films have been used extensively as biosensing platforms. These films exhibit certain similarities with cell membranes, thus providing a suitable means for the immobilization of proteinaceous moieties and, further, a number of intrinsic signal amplification mechanisms. Their implementation in the detection of toxins yielded reliable and fast detectors for in field analyses of environmental and clinical samples. Some examples are presented herein, including aflatoxin and cholera toxin detection. The conditions and parameters that determine the analytical specifications of the lipid membrane sensors are discussed, advantages and technology bottlenecks are reviewed, and possible further developments are highlighted.

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“…This phenomena was used as the basis for the investigation of generic transduction of hydrolytic enzyme reactions where hydronium ions generated at the surface of BLMs caused dynamic alterations of the electrostatic fields of such membranes [2, 31. Recent work involving titrations of the acidic lipid constituent on one side of a BLM provided evidence which suggested that a rearrangement of the electrical double layer would not be the only phenomenon responsible for the transient signals that were observed [23]. The charging of the electrical double layer in BLMs was related to a significant structural transition which occurred rapidly as the pH was gradually changed.…”

mentioning

confidence: 97%

Stabilized bilayer lipid membranes for flow‐through experiments

et al. 1995

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This work reports a technique for the stabilization of solventless bilayer lipid membranes (BLMs), and the use of stabilized BLMs as flow detectors. Microporous filters composed of glass fibers, polytetrafluoroethylene (PTFE) and polycarbonate (nominal pore sizes from 1 to 5 pm) can serve as interfaces that separate two solution compartments. The micropores in the filter media can act as supports for formation and stabilization of BLMs. One of the solution compartments is used to cast lipid films on the filters, while a carrier electrolyte solution concurrently flows through the opposing compartment. Optimization of the flow cell design, and the chemical composition and methods for preparation of stabilized BLMs, are described. Lipid membranes composed of mixtures of phosphatidyl choline and phosphatidic acid could respond rapidly to pH alterations of the carrier electrolyte solution. Signals would reproducibly appear within a few seconds following the injection of an electrolyte of different pH than the carrier. Signals took the form of a single ion current transient with magnitude of tens of picoamperes (PA) and a duration of seconds. The mechanism of signal generation is explored by differential scanning calorimetry. The results show that a phase transition within a lipid membrane can be triggered by pH alterations of the electrolyte solution. Stabilized BLMs which provide artificial ion gating events hold prospects for chemical sensing of process streams.

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Bilayer lipid membranes as electrochemical switches in reactions involving alteration of surface charge

Cited by 13 publications

References 12 publications

Stabilized Lipid Membrane Based Biosensors with Incorporated Enzyme for Repetitive Uses

Stabilized Lipid Membrane Based Biosensors with Incorporated Enzyme for Repetitive Uses

Application of Biosensors Based on Lipid Membranes for the Rapid Detection of Toxins

Stabilized bilayer lipid membranes for flow‐through experiments

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