Bilateral Ovariectomy in the Mare

Ragle, Claude A.

doi:10.1002/9781118704875.ch17

Cited by 3 publications

(4 citation statements)

References 16 publications

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“…Although evisceration could still be a risk, 26 the authors feel that the risk was minimal due to the small size of the vaginotomy site and the relatively quick healing observed on vaginoscopy. After bilateral ovariectomy using a traditional laparoscopic approach, painful reaction to palpation and swelling of the portal sites could be expected during the first 3–5 postoperative days 30 ; however, this complication was not subjectively apparent in the mares in this study.…”

Section: Discussionmentioning

confidence: 59%

Hybrid natural orifice transluminal endoscopy surgery (NOTES) to perform bilateral ovariectomy in mares

et al. 2021

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Objectives To combine laparoscopic techniques with natural orifice transluminal endoscopic surgery (hybrid NOTES) to minimize number and enlargement of paralumbar laparoscopic portals for bilateral ovariectomy in standing, sedated mares. Study design Case series. Animals Six horses. Materials and Methods Six mares with palpably normal ovaries were restrained in stocks, sedated, and had caudal epidural anesthesia performed. A 7.5 MHz ultrasound probe was used transvaginally to select placement of vaginotomy. An attempt was made to remove both ovaries with 70 cm esophageal forceps through the vaginotomy, while visualized and transected via ipsilateral paralumbar fossa laparoscopic portals. Surgical time, intraoperative, and postoperative complications were recorded. Vaginoscopy was performed at days 0, 3, 7, and 14 postoperatively. After 14 days, mares were euthanized and necropsied. Results Hybrid NOTES allowed successful bilateral ovariectomy in all six mares, with a mean surgical time of 70 ± 25 min. In two mares, one ovary was dislodged from the forceps during vaginotomy extraction. Abdominal retrieval of the ovary was successful with reapplication of forceps or a surgeon's hand via vaginotomy. At necropsy, five mares had no adhesions within the abdominal cavity or at the vaginotomy site. One mare in which hand retrieval of the ovary was necessary, developed an adhesion between the bladder and the vaginotomy. Conclusions This technique appears to offer a safe alternative for bilateral ovariectomy with minimal postoperative complications. Clinical Significance Decreased number and size of paralumbar laparoscopic incisions could decrease complications associated with exteriorization of ovaries through the flank.

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Section: Discussionmentioning

confidence: 59%

Hybrid natural orifice transluminal endoscopy surgery (NOTES) to perform bilateral ovariectomy in mares

et al. 2021

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“…27,28 Entry to the abdomen was established at the left last intercostal space because it is a safe, reliable location, where the peritoneum is easily penetrated and not displaced. 24 This port was placed with minimal extra time, allowed inspection of the left side, and provided visual guidance to place the right ports. The authors had previously found the arrangement of the right ports to be the easiest layout for small intestinal exploration in most horses.…”

Section: Discussionmentioning

confidence: 99%

“…One port was created in the left last intercostal space at the level of the base of the tuber coxae. 24 A second port was placed in the center of the paralumbar fossa on the right side at the level of the middle of the tuber coxae. Two ports were created approximately 8-10 cm ventral to the second port, aligned on the same horizontal level, thereby forming a triangle.…”

Section: A T E R I a L S A N D Me T H O D Smentioning

confidence: 99%

Laparoscopic evaluation of the small intestine in the standing horse: Technique and effects*

et al. 2017

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“…Following a 12‐hr fast, the females were submitted to a bilateral ovariectomy procedure by video laparoscopy (Ragle, ). Pairs of ovaries ( n = 10) were collected and transported from the surgical centre to the laboratory in minimum essential medium (MEM) at room temperature within 30 min, and washed once in 70% alcohol for 10s and twice with phosphate‐buffered saline (PBS).…”

Section: Methodsmentioning

confidence: 99%

Vitrification of ovarian tissue of Brazilian North‐eastern donkeys (Equus asinus) using different cryoprotectants

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Praxedes

Campos

et al. 2018

Reprod Domestic Animals

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The aim of this study was to assess a vitrification protocol for asinine ovarian tissue, to preserve preantral follicles using different cryoprotectant solutions, composed of various concentrations (EG 3 M or 6 M) of dimethyl sulfoxide or ethylene glycol isolate, or as a combination (DMSO 3 M + EG 3 M). Ten pairs of ovaries from Brazilian north-eastern breed jennies were obtained through videolaparoscopy, and cortical fragments were submitted to a solid-surface vitrification (SSV) using each cryoprotectant solution. The ovarian tissue was evaluated for follicular morphology and viability, DNA integrity (TUNEL technique) and the presence of nucleolar organizing regions in granulosa cells (AgNOR technique). After thawing, the percentage of normal preantral follicles was significantly reduced in the vitrified ovarian tissue fragments compared to the fresh control (p < 0.05). When comparing treatments, the use of DMSO 3 M (81.7 ± 37.5%), EG 3 M (83.7 ± 27.4%) and the combination of both DMSO 3 M + EG 3 M (81.8 ± 46.8%) allowed a greater percentage of follicular survival in contrast to DMSO 6 M (69.8 ± 16.5%) and EG 6 M (72.3 ± 18.0%; p < 0.05). When vitrified using the DMSO + EG combination, a higher percentage (62.5 ± 29.1%) of viable follicles (trypan blue) was observed in relation to the other vitrification treatments (p < 0.05). The TUNEL technique identified that all treatments tested showed DNA fragmentation in the follicular cells, except in the case of the DMSO 3 M + EG 3 M treatment. When evaluating the presence of NORs, no significant differences were observed in the amount of NORs between the fresh and vitrified groups using DMSO 3 M + EG 3 M (p > 0.05). We concluded that the combination DMSO 3 M + EG was more efficient for the vitrification of ovarian tissue taken from Equus asinus, allowing adequate preservation of PAFs morphology, viability, DNA integrity and cell proliferative capacity.

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Bilateral Ovariectomy in the Mare

Cited by 3 publications

References 16 publications

Hybrid natural orifice transluminal endoscopy surgery (NOTES) to perform bilateral ovariectomy in mares

Hybrid natural orifice transluminal endoscopy surgery (NOTES) to perform bilateral ovariectomy in mares

Laparoscopic evaluation of the small intestine in the standing horse: Technique and effects*

Vitrification of ovarian tissue of Brazilian North‐eastern donkeys (Equus asinus) using different cryoprotectants

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