Introduction:The pancreas and male gonads shared similar utilities. Aim: To follow-up the pancreatic structural alterations after orchiectomy on different times. Materials and Methods: Twenty-four rats were allocated into sham-operated (Group I, n=6) and orchiectomized (Orx) (Group II, n=18). After 2 weeks of rest, the Gr II was equally re-distributed into subgroups IIa, IIb, and IIc depending on sacrifice time (3, 6 and 9 weeks, respectively). The pancreatic specimens were processed and stained with H&E and were immunostained with anti-insulin and glucagon proteins and anti-single-strand DNA (ss-DNA). Body masses, serum testosterone, fasting plasma glucose levels, oxidant and antioxidant markers were measured. The optical density of insulin immunopositive reaction and immunopositive area % of insulin, glucagon, and ss-DNA were realized with image analyzer. Data were statistically analyzed by ANOVA's and Tukey's test.Results: This study revealed that the orchiectomized rats were hyperglycemic. The oxidative stress was significantly elevated while the antioxidant enzyme was significantly decreased. Structurally, in Gr IIa, the acini were deformed and shrunken. The acinar cells had less basophilic cytoplasm with small and darkly basophilic nuclei, while the islets cells appeared deeply acidophilic with small darkly stained nuclei. The apoptotic cells were observed in some acinar cells and in cells peripherally located in islets. The positive area% of the β cell was non-significantly decreased, whereas the positive area% of α cell was significantly decreased. Additionally, the positive area% of apoptotic cells was significantly increased. Gr IIb and GrIIc revealed the same structural changes as in GrIIa. Conclusion: Orchiectomy-induced testosterone deficiency affects the exocrine and endocrine pancreatic portions, both with a risk for pancreatogenic diabetes (type III). Therefore, we should be more cautious before performing orchiectomy in humans or animals.