Bilateral Entry and Release of Middle East Respiratory Syndrome Coronavirus Induces Profound Apoptosis of Human Bronchial Epithelial Cells

Tao, Xinrong; Hill, Terence E.; Morimoto, Chikao; Peters, C. J.; Ksiazek, Thomas G.; Tseng, Chien Te K.

doi:10.1128/jvi.01562-13

Cited by 54 publications

(56 citation statements)

References 18 publications

(19 reference statements)

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“…This finding is consistent with a localized infection restricted in the intestine. Several studies have investigated the interaction of coronaviruses and other viruses with epithelial cells using a similar approach (Ren et al, 2006;Rossen et al, 1994;Wang et al, 2000;Jia et al, 2005;Tseng et al, 2005;Rossen et al, 2001Rossen et al, , 1995Rossen et al, , 1996Pratelli, 2011;Tao et al, 2013;Fuller et al, 1984). Though entry into and egress from polarized cells for several coronaviruses is restricted to the apical plasma membrane, other coronaviruses show a different pattern (see Introduction).…”

Section: Discussionmentioning

confidence: 99%

“…Feline coronavirus (FCoV) and mouse hepatitis coronavirus (MHV) mediated apical entry and basolateral release in polarized epithelial cells (Rossen et al, 2001(Rossen et al, , 1995(Rossen et al, , 1996. The recently identified coronavirus, Middle East Respiratory Syndrome Coronavirus (MERS-CoV), and also the canine coronavirus (CCoV) enter and exit at both sites of polarized epithelial cells (Pratelli, 2011;Tao et al, 2013). These examples show that coronaviruses have evolved different ways to interact with polarized cells.…”

Section: Introductionmentioning

confidence: 99%

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Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells

Cong

Bai

et al. 2015

Virology

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a b s t r a c tInfection of polarized intestinal epithelial cells by porcine epidemic diarrhea virus (PEDV) was characterized. Indirect immunofluorescence assay, real-time PCR, and transmission electron microscopy confirmed PEDV can be successfully propagated in immortalized swine small intestine epithelial cells (IECs). Infection involved porcine aminpeptidase N (pAPN), a reported cellular receptor for PEDV, transient expression of pAPN and siRNA targeted pAPN increased and decreased the infectivity of PEDV in IECs, respectively. Subsequently, polarized entry into and release from both Vero E6 and IECs was analyzed. PEDV entry into polarized cells and pAPN grown on membrane inserts occurs via apical membrane. The progeny virus released into the medium was also quantified which demonstrated that PEDV is preferentially released from the apical membrane. Collectively, our data demonstrate that pAPN, the cellular receptor for PEDV, mediates polarized PEDV infection. These results imply the possibility that PEDV infection may proceed by lateral spread of virus in intestinal epithelial cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells

Cong

Bai

et al. 2015

Virology

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“…The MERS (Middle East respiratory syndrome)-CoV, in contrast, can enter and exit cells through both apical and basolateral membranes [247], and viral release occurs through inducing apoptosis in the infected cell, but no specific interactions with the polarity proteins have been reported.…”

Section: Coronavirusesmentioning

confidence: 99%

Upsetting the Balance: When Viruses Manipulate Cell Polarity Control

Thomas

Banks

2018

Journal of Molecular Biology

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The central importance of cell polarity control is emphasized by the frequency with which it is targeted by many diverse viruses. It is clear that in targeting key polarity control proteins, viruses affect not only host cell polarity, but also influence many cellular processes, including transcription, replication, and innate and acquired immunity. Examination of the interactions of different virus proteins with the cell and its polarity controls during the virus life cycles, and in virally-induced cell transformation shows ever more clearly how intimately all cellular processes are linked to the control of cell polarity.

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“…The observation that HPAI [11] and pandemic H1N1 often induce necrosis in epithelial cells, while seasonal H3N2 appears only to cause it in immune compromised hosts [48], may at least partly explain the association between severity of disease and necrosis of the epithelium. Moreover, other strains of highly pathogenic viruses such as dengue virus [74] or MERS-CoV [75] appear to induce considerable necrosis in epithelial cells to promote viral replication and propagation, while less virulent viruses like rhinovirus, or SARS-CoV induce limited or no necrosis [76,75]. In vitro, H1N1 IAV induces necrosis in human lung bronchiolar epithelial cells, coupled with massive release of proinflammatory cytokines and neutrophil chemoattractant CXCL8, which is highly suggestive of a link between necrosis of the epithelium and ARDS [77].…”

Section: Necrosis In Iav-infected Epithelial Cellsmentioning

confidence: 99%

Dissecting host cell death programs in the pathogenesis of influenza

Downey

Pernet

François

et al. 2018

Microbes and Infection

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Influenza A virus (IAV) is a pulmonary pathogen, responsible for significant yearly morbidity and mortality. Due to the absence of highly effective antiviral therapies and vaccine, as well as the constant threat of an emerging pandemic strain, there is considerable need to better understand the host-pathogen interactions and the factors that dictate a protective versus detrimental immune response to IAV. Even though evidence of IAV-induced cell death in human pulmonary epithelial and immune cells has been observed for almost a century, very little is known about the consequences of cell death on viral pathogenesis. Recent study indicates that both the type of cell death program and its kinetics have major implications on host defense and survival. In this review, we discuss advances in our understanding of cell death programs during influenza virus infection, in hopes of fostering new areas of investigation for targeted clinical intervention.

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Bilateral Entry and Release of Middle East Respiratory Syndrome Coronavirus Induces Profound Apoptosis of Human Bronchial Epithelial Cells

Cited by 54 publications

References 18 publications

Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells

Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells

Upsetting the Balance: When Viruses Manipulate Cell Polarity Control

Dissecting host cell death programs in the pathogenesis of influenza

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