BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin.

Abstract

Abstract. BIK1 function is required for nuclear fusion, chromosome disjunction, and nuclear segregation during mitosis. The BIK1 protein colocalizes with tubulin to the spindle pole body and mitotic spindle. Synthetic lethality observed in double mutant strains containing a mutation in the BIK1 gene and in the gene for ~-or/~-tubulin is consistent with a physical interaction between BIK1 and tubulin. Furthermore, over-or underexpression of BIKI causes aberrant microtubule assembly and function, bikl null mutants are viable but contain very short or undetectable cytoplasmic microtubules. Spindle formation often occurs strictly within the mother cell, probably accounting for the many multinucleate and anucleate bikl cells.

Microtubule‐associated coiled‐coil protein Ssm4 is involved in the meiotic development in fission yeast

“…Glued is a likely homologue of DP-150 in Drosophila melanogaster (Holzbaur et al 1991). BIK1 is required for microtubule functions during mitosis and conjugation in Saccharomyces cerevisiae, and has been shown to colocalize with microtubules by immunofluorescence microscopy (Berlin et al 1990). Thus, we suspected that Ssm4 might be a novel microtubule-associated protein of S. pombe.…”

Thioredoxin is required for vacuole inheritance in Saccharomyces cerevisiae.

“…Organelle inheritance has also been studied in Saccharomyces cerevisiae (Yaffe, 1991). The yeast nuclear membrane does not vesiculate during cell division ("closed mitosis"), and nuclear inheritance is blocked in mutants affecting the spindle (Berlin et al, 1990;Huffacker, 1992;Palmer et al, 1992;Eshel et al, 1993;Clark and Meyer, 1994;Muhua et al, 1994). Mutants have also been described in mitochondrial inheritance (mdm mutants), some affecting homologs of intermediate filament proteins Yaffe, 1992, 1993).…”

The evolutionary conserved BER1 gene is involved in microtubule stability in yeast

“…6). This phenotype is not common, since most of the mutants of factors aVecting microtubules such as Bim1, Bik1 and Stu2 are more sensitive to such drugs (Al-Bassam et al 2006;Berlin et al 1990;Schwartz et al 1997;Winsor and Schiebel 1997).…”