Biglycan protects cardiomyocytes against hypoxia/reoxygenation injury: Role of nitric oxide

The role of novel and known extracellular matrix and adhesion molecules in the homeostatic and regenerative bone marrow microenvironment

“…224 Biglycan induces nitric oxide synthases, thereby protecting cells from hypoxia and reoxygination injury. 225 Biglycan expression correlates with the malignancy of colorectal and gastric cancer. 226,227 Biglycan is high expressed in tumor endothelial cells and is required for endothelial cell migration.…”

Novel approaches of pharmacological preconditioning: the role of biglycan and miRNAs

“…Consistent with these cardioprotective effects, our research group has previously demonstrated that exogenously administered biglycan could protect neonatal cardiac myocytes against simulated ischemia/reperfusion (SI/R) injury [68]. Although these findings suggest that biglycan exerts cardioprotective effects, the exact molecular mechanism behind this phenomenon has not yet been elucidated.…”

“…In pilot experiments, we tested whether the core protein obtained by enzymatic removal of GAG chains from biglycan induces cytoprotection using the same experimental protocol as in our earlier study [68]. Two days old cardiomyocyte cultures were treated with 0, 1, 3, 10, 30, 100 nM native (glycanated) or deglycanated biglycan (dissolved in DMEM + 1% FBS) for 20 h, and were subjected to 150 min simulated ischemia (SI)…”

“…followed by 120 min simulated reperfusion as described previously ( Figure 5) [68]. Then the cells were transferred into a normoxic incubator and covered with differentiation medium.…”

Cytoprotective approaches to protect myocardium against ischemia/reperfusion injury

“…could influence the action of matrix metalloproteinases [61,62]. Cell isolation, culturing method and the SI/R drug screening platform were described before in detail [58,63]. Briefly, neonatal rats were sacrificed by cervical dislocation and hearts were cell/well.…”