Big Losses Due to Bacterial Contaminations Can Be Avoided in Mass Propagation Scheme.

Boxus, P.; Terzi, J.-M.

doi:10.17660/actahortic.1987.212.12

Cited by 33 publications

(24 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On the contrary, if no indexing procedures are employed, evidence of bacterial growth is important so that contaminated cultures may be detected and discarded. Boxus & Terzi (1987) recommended the incorporation of peptone and yeast extract into a proliferation media for wild cherry to promote turbidity caused by latent contaminants so that they can be identified and removed. Bacterial multiplication was rapid when bacteria were added in low concentrations to undifferentiated anthurium callus tissue.…”

Section: Resultsmentioning

confidence: 99%

Latent infections of in vitro anthurium caused by Xanthomonas campestris pv. dieffenbachiae

Norman

Alvarez

1994

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Latent infections of tissue-cultured Anthurium andraeanum Lind. caused by the blight pathogen, Xanthomonas campestris pv. dieffenbachiae (McCulloch & Pirone) Dye, were examined. The pathogen survived in or on callus for over 4 months without producing symptoms in callus or turbidity in the medium. The pathogen survived for more than 1 year on or within stage II shoots without producing symptoms and was successively transferred three times as latently infected shoots were multiplied. The pathogen did not grow or survive for more than 2 weeks in Murashige and Skoog medium lacking plant material. The addition of coconut water enhanced bacterial growth and produced turbidity in culture media. Latently infected in vitro anthuriums may be inoculum sources for subsequent outbreaks of the disease.

show abstract

Section: Resultsmentioning

confidence: 99%

Latent infections of in vitro anthurium caused by Xanthomonas campestris pv. dieffenbachiae

Norman

Alvarez

1994

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…At the end of several of the experiments, regenerated shoots were removed from leaf explants and placed on shoot multiplication medium consisting of DKW as formulated above with 3.0 IxM benzyladenine (BA). In addition, 265 mg 1-1 peptone (Difco, Detroit, MI, USA) and 88 mg 1-l yeast extract (Difco) were added to the multiplication medium to help detect any bacterial contamination (Boxus & Terzi 1987).…”

Section: Methodsmentioning

confidence: 99%

Cytokinins, donor plants and time in culture affect shoot regenerative capacity of American elm leaves

George

Tripepi

1994

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Cytokinins, donor plants and their time in vitro as well as basal media were investigated for their influence on shoot regenerative capacity of American elm (Ulmus americana L.) leaves. Leaves excised from six 2-year-old seedlings formed adventitious shoots when placed on Driver and Kuniyuki Walnut (DKW) medium supplemented with 7.5, 15 or 22.5 laM of benzyladenine (BA) or thidiazuron (TDZ). Thidiazuron induced significantly higher regeneration percentages on elm leaves than BA, regardless of concentration used. Donor plant also affected the efficiency of shoot regeneration, with certain seedlings having 1.5 to 7 times more explants forming shoots as compared to other seedlings tested. By subculture 15, the average number of shoots per regenerating explant increased at least 3-fold for leaves on media with BA or TDZ for the one donor plant that survived continued subculturing. Leaf explants from donor plants with the highest regenerative capacity had a higher percentage of shoot formation on DKW than MS medium. Explants from productive donor plants should be placed on DKW medium supplemented with TDZ to improve shoot regeneration efficiency from American elm leaves.

show abstract

“…Plant tissue culture has had a great impact on the improvement of quality and quantity of commercially important cultivated crops and ornamental plants (Serageldin, 1999;Valdez, López & Jiménez, 2004). However, microbial contamination of the plantlets cultivated in vitro has caused major losses in production, ranging from 3% to 55% (Boxus & Terzi, 1987;Leifert, Morris & Waites, 1994). Viruses, bacteria, yeasts, fungi, mites, and thrips are among the major contaminants of vegetable tissues (Leifert & Cassells, 2001).…”

mentioning

confidence: 99%

Use of different SEM techniques in the study of Tyrophagus putrescentiae (Acari: Acaridae) in Costa Rica

Murillo

Aguilar

Sánchez

2013

URJ

View full text Add to dashboard Cite

Scanning electron microscopy (SEM) has been used as a tool to facilitate the morphological description of mites and to complement the information obtained from the light microscope. Unfortunately, several of SEM techniques used for soft-bodied mites such as Tyrophagus, have in many cases not shown the desired results. This investigation sought to develop an efficient procedure for preparing specimens of T. putrescentiae for SEM so that its major morphological characters could be observed and photographed. This species is the most important contaminant of tissue culture laboratories in Costa Rica. Seven methods were used to process the samples. The evaluated treatments showed differences in their ability to preserve the morphological structures of the specimens. The advantages and disadvantages of each treatment are discussed. Mites processed using ethanol were the most viable option with method 3 being the procedure that showed the best results; this technique also reduced the time and costs of processing the specimens. KEY WORDSAcari, Acaridae, Tyrophagus putrescentiae, Scanning Electron Microscopy (SEM), SEM techniques RESUMEN El microscopio electrónico de barrido (MEB) ha sido utilizado como una herramienta para complementar la información obtenida bajo el microscopio de luz. Desafortunadamente, muchas de las técnicas utilizadas con el MEB para ácaros de cuerpo suave como Tyrophagus, no han mostrado los resultados deseados en muchos casos. El presente trabajo buscó un procedimiento eficiente para observar y fotografiar bajo MEB los caracteres morfológicos más importantes de T. putrescentiae. Esta especie es el principal contaminante en cultivo de tejidos vegetales en Costa Rica. Se utilizaron siete tratamientos para el procesamiento de las muestras. Los tratamientos evaluados mostraron diferencias entre sí con respecto a la preservación de las estructuras de los especímenes. Se discuten las ventajas y desventajas de cada procedimiento. Los tratamientos con etanol fueron la opción más viable para procesar estos ácaros, siendo el tratamiento 3 el que mostró mejores resultados. Asimismo, esta técnica permite reducir tanto el tiempo como los costos durante el procesamiento de los especímenes. PALABRAS CLAVEAcari, Acaridae, Tyrophagus putrescentiae, Microscopio Electrónico de Barrido (MEB), Técnicas MEB

show abstract

Big Losses Due to Bacterial Contaminations Can Be Avoided in Mass Propagation Scheme.

Cited by 33 publications

References 0 publications

Latent infections of in vitro anthurium caused by Xanthomonas campestris pv. dieffenbachiae

Latent infections of in vitro anthurium caused by Xanthomonas campestris pv. dieffenbachiae

Cytokinins, donor plants and time in culture affect shoot regenerative capacity of American elm leaves

Use of different SEM techniques in the study of Tyrophagus putrescentiae (Acari: Acaridae) in Costa Rica

Contact Info

Product

Resources

About