The bifurcation problems of twisted heteroclinic loop with two hyperbolic critical points are studied for the case [Formula: see text], [Formula: see text], [Formula: see text], where [Formula: see text] and [Formula: see text] are the pair of principal eigenvalues of unperturbed system at the critical point [Formula: see text], [Formula: see text]. Under the transversal conditions, the authors obtained some results of the existence and the number of 1-homoclinic loop, 1-periodic orbit, double 1-periodic orbit, 2-homoclinic loop and 2-periodic orbit. Moreover, the relative bifurcation surfaces and the existence regions are given, and the corresponding bifurcation graphs are drawn.